@article{ajmr2015332,
author={{Osman, Najem Aldin M. and Alrayah, Intisar E. and Mohamed, Yassir Mahgoub and El-Eragi, Ali M and Eldirdery, Muataz M. and Salih, Mohamed Ahmed},
title={Molecular Study of Panton-Valentine Leukocidin Genes among<i> Staphylococcus</i><i> aureus</i> Clinical Isolates in Khartoum State, Sudan},
journal={American Journal of Microbiological Research},
volume={3},
number={3},
pages={107--111},
year={2015},
url={http://pubs.sciepub.com/ajmr/3/3/2},
issn={2328-4137},
abstract={<i>Staphylococcus aureus</i> strains carrying Panton- Valentine Leukocidin genes (PVL) are an emerging threat worldwide, causing variety of infections even in healthy individuals. Intensive efforts through the last years have been carried out towards the detection and analysis of PVL genes. The prevalence and characterization of such genes has not been done in Sudan. In this study we investigated the prevalence and the molecular characteristics of PVL genes among <i>S. aureus</i> clinical isolates, comparing their PVL allelic variant with that of PVL positive strains from different countries. Standard microbiological procedures were used for the identification of isolates, polymerase chain reaction for determination of PVL genes and standard sequencing for mapping of <i>lukS/F</i>-PV genes. In Silico tools were used for sequence analysis. Among 210 <i>S. aureus</i> isolates, PVL genes were detected in 122 (58%). Sequence analysis for <i>lukS/F</i>-PV genes from 12 representative isolates detected a new point mutation in <i>lukS</i> PV region. Collectively, our findings showed a high frequency of PVL genes among <i>S. aureus</i> isolates and revealed a novel nonsynonymous mutation. Phylogenetic analysis revealed that Sudanese isolates were closely related to each other.},
doi={10.12691/ajmr-3-3-2}
publisher={Science and Education Publishing}
}