@article{ajmr2014268,
author={{Altayb, Hisham N and Amin, Nagwa M El and Mukhtar, Maowia M. and Salih, Mohamed Ahmed and Siddig, Mohamed A M},
title={Molecular Characterization and in Silico<i> </i>Analysis of a Novel Mutation in TEM-1 Beta-Lactamase Gene among Pathogenic <i>E. coli </i>infecting a Sudanese Patient},
journal={American Journal of Microbiological Research},
volume={2},
number={6},
pages={217--223},
year={2014},
url={http://pubs.sciepub.com/ajmr/2/6/8},
abstract={The presence of ESBLs in many <i>E. coli </i>strains are of serious concern, since these organisms are the most common cause of different human infections. In this study we isolate an<i> E. coli</i> bacterium with high hydrolytic activity against cefotaxime. The ESBLs production was confirmed by phenotypic confirmatory test, while the ESBLs genes were detected by polymerase chain reaction (PCR). This isolate was positive for TEM gene and negative for CTX-M and SHV genes. DNA sequencing was done for TEM gene. The nucleotide sequences and translated proteins were subjected to BLAST for sequences similarity and homology, BLASTp result revealed a substitution of aspartic acid in TEM-1(gb: AFI61435.1) to Threonine at position 262. In Silico tools was used for mutation analysis and prediction of secondary and tertiary structure of wild and mutant type genes. We conclude that our mutant gene is completely different from the wild types TEM-1 gene, within phenotypic and genotypic levels. So we conclude a novel mutant TEM gene with ESBLs activity is been detected in Sudan Phylogenetic tree revealed that the possible source of our gene is Iran.},
doi={10.12691/ajmr-2-6-8}
publisher={Science and Education Publishing}
}