@article{ajmr2014267,
author={{EL-Sherbiny, Gamal M. and E., Shehata M.},
title={Antimicrobial Susceptibility, Heavy Metals Tolerance and Plasmid Curing of <i>Shigella</i> Species Isolated from  El- Dakahlia, Egypt},
journal={American Journal of Microbiological Research},
volume={2},
number={6},
pages={211--216},
year={2014},
url={http://pubs.sciepub.com/ajmr/2/6/7},
abstract={The aim of this study was carried out determine the antibiotic susceptibility, heavy metals tolerance and plasmid curing of <i>Shigella</i> species isolating from diarrheal stool samples were collected from different hospitals in El- Dakahlia and Nile River waters, Egypt, from January 2012 to August 2014. After characterization and identification the results obtained show that 172 isolates isolated from stool samples belong to four <i>Shigella</i> species (<i>Shigella</i> <i>sonnei</i> 48.25 %, <i>Shigella flexneri</i> 29.65 %, <i>Shigella dysenteriae</i> 13.95 % and <i>Shigella boydii </i>8.13 %) while 5 isolates isolated from Nile River water were found belong to (<i>Shigella sonnei </i>40%,<i> Shigella dysenteriae </i>40 %<i> </i>and <i>Shigella flexneri</i> 20 %).The antibiotics susceptibility of <i>Shigella</i> sp. to11 antibiotics revealed that the most potent antibiotics were found co-amoxyclav, ciprofloxacin and ceftriaxone respectively while penicillin, ampicillin, co-trimoxazole and chloramphenicol respectively give low activity. The tolerance of<i> Shigella</i> sp. to heavy metals, (cadmium, nickel cobalt and zinc) revealed that all isolates sensitive to 1 and 0.1M concentration. Plasmid profile analysis of ten isolates <i>Shigella</i> <i>sonnei </i>shown that this isolates having numerous plasmid ranged from 8.5 to 4.3 kb. Treat isolates with SDS 2% for 24 hours to plasmid curing after recovery subject to antibiotic sensitivity and heavy metals tolerance. In conclusion, <i>Shigella</i>-associated diarrhea remains relatively common in Egypt and can be used ciprofloxacin and ceftriaxone for treat <i>Shigella</i> sp. infection. The heavy metal tolerance of <i>Shigella</i> sp .associated with resistance to antibiotics ampicillin, tetracycline and chloramphenicol. Present <i>Shigella</i> sp. in Nile River waters indicates polluted with sewage waters and becomes sources of some epidemic diseases.},
doi={10.12691/ajmr-2-6-7}
publisher={Science and Education Publishing}
}