@article{ajmr2014265,
author={N., OBI C.},
title={Bacteriological Assessement of Vegetables Cultivated in Soils Treated with Poultry Manure and the Manure-Treated Soil Samples},
journal={American Journal of Microbiological Research},
volume={2},
number={6},
pages={189--200},
year={2014},
url={http://pubs.sciepub.com/ajmr/2/6/5},
abstract={Bacteriological quality of four vegetables: waterleaf (W), pumpkin (P), cucumber (C) and tomatoes (T) cultivated in soils treated with poultry manure and the manure-treated soil samples (S1, S2, S3 and S4) were assessed using standard microbiological methods. <i>Escherichia coli</i>, <i>Staphylococcus aureus</i>, <i>Klebsiella </i>spp<i>.,</i> <i>Bacillus </i>spp, <i>Salmonella </i>spp. and <i>Shigella </i>spp were recovered from the soil and vegetable samples. The total bacterial count of the soil samples ranged from 1.75กม10<SUP>9</SUP> (S3) to 5.9กม10<SUP>4</SUP>cfu/ml (S4) while the total bacterial count for the vegetable samples ranged from 2.65กม10<SUP>9</SUP> (P) to 1.50กม10<SUP>5</SUP>cfu/ml (W). The total coliform count of the soil samples ranged from 1.65กม10<SUP>7</SUP>(S3) to 6.9กม10<SUP>4</SUP>cfu/ml (S4) while that of the vegetable samples ranged from 1.20กม10<SUP>8</SUP>(P) to 7.2กม10<SUP>5</SUP>cfu/ml (W). S1 was significantly different from S2 and S4 for the total bacteria count for the soil samples while P and W were significantly different for the vegetable samples (P&lt; 0.05). For <i>Salmonella</i>-<i>Shigella</i> plate counts, S1 and S4 were significantly different and S2 and S3 were same (P&lt; 0.05); while for the vegetable samples, P, W, C and T were significantly different (P&lt; 0.05). S1, S2, S3, and S4 were significantly different for the total coliform count for the soil samples and same for P, W and C (P&lt;0.05). Sensitivity screening for the isolates showed that <i>Bacillus </i>spp<i> </i>was most sensitive to Ofloxacin (25mm) while <i>Staphylococcus aureus</i> was most sensitive to Ofloxacin (22mm). <i>Shigella</i> was most sensitive to Ciprofloxacin (25mm) while <i>E.</i><i> </i><i>coli</i> showed highest sensitivity to Ciproflacin (25mm) and Ofloxacin (25mm). In addition, <i>Klebsiella</i> spp was most sensitive to Ciprofloxacin (23mm) and Ofloxacin (23mm) and <i>Salmonella</i> showed resistance to all the antibiotics. This study demonstrated that there is a high level of microbial contamination associated with the cultivation of vegetables in soils in which organic manure has been applied to which is of risk to the consumers.},
doi={10.12691/ajmr-2-6-5}
publisher={Science and Education Publishing}
}