@article{ajmr2014234,
author={{Kumar, G. Kranthi and Ram, M. Raghu},
title={Phosphate Solubilizing Rhizobia Isolated from <i>Vigna trilobata</i>},
journal={American Journal of Microbiological Research},
volume={2},
number={3},
pages={105--109},
year={2014},
url={http://pubs.sciepub.com/ajmr/2/3/4},
abstract={Rhizobial strains were isolated from root nodules of <i>Vigna trilobata</i> plants raised in soils of different districts of Andhra Pradesh, India. Among the 23 strains of rhizobia isolated, 6 strains were proved to be positive for phosphate solubilization. The strains were identified as <i>Sinorhizobium</i> sp. strain MRR101-KC428651, <i>Agrobacterium tumefaciens</i> strain MRR102- KC428652, <i>Rhizobium</i> sp. strain 103 ¨C JX576499 ; <i>Sinorhizobium kostiense</i> strain MRR104- KC428653; <i>Agrobacterium tumefaciens</i> strain MRR105- KC428654 and <i>Rhizobium</i> sp. strain MRR106- KC428655<b> </b>after 16S rDNA sequencing. <i>S. kostiense</i> strain MRR104 was found to be better than strains of <i>Agrobacterium tumefaciens</i> and <i>Rhizobium</i> sp. in phosphate solubilization with maximum zone of solubilization (18mm) on standard Pikovskaya¡¯s medium and with maximum P<SUB>2</SUB>O<SUB>5</SUB> liberation of 510 ¦Ìg/ml in liquid medium. The optimization for maximum phosphate solubilization was done by using different carbon and nitrogen sources. Glucose was preferred as carbon source by all the strains studied, with 10 fold increase in solubilization of phosphorous than other carbon sources. Phosphate solubilization increased with increase in concentration of glucose up to 3% in all the strains studied. Some strains preferred ammonium sulphate and others preferred nitrates as nitrogen source for phosphate solubilzation, indicating that strains of <i>V. trilobata</i> are adopting two different mechanisms for solubilization. Reduction in pH with increased phosphate solubilization efficiency was also observed among the strains, irrespective of the carbon sources tested. Strain <i>S. kositense </i>MRR104 was proved to the better strain with maximum libration of phosphorous along with maximum reduction in pH as 5.13. <i>Sinorhizobium</i> strains performed better than that of <i>Rhizobium</i> and <i>Agrobacterium</i> in solubilization of phosphorous.},
doi={10.12691/ajmr-2-3-4}
publisher={Science and Education Publishing}
}