eng Science and Education Publishing American Journal of Microbiological Research 2014-03-16 2 2 57 59 10.12691/ajmr-2-2-2 AJMR2014222 article Padmajakshi. G 1 S. Saini 1 Sachin deorukhkar 1 K V Ramana ramana_20021@rediffmail.com 2 Department of Microbiology, Pravara Medical College, Loni, Maharashtra, India Department of microbiology, Prathima Institute of Medical Sciences, Karimnagar, India Candida spp. is most common opportunistic pathogen in HIV/AIDS patients causing severe and rapidly progressive, difficult to diagnose and often refractory to therapy. In the era of emergence of resistance among different microorganisms due to various factors, Candida spp are among those microbes that have to be extensively studied in the areas of pathogenic and virulence determinants. Coagulase emzyme is considered as a virulence factor in Staphylococci and that has been studied least in Candida spp as evidenced by available literature. We have attempted to evaluate the coagulase activity among Candida spp isolated from HIV seropositive patients. This prospective study included 179 Candida isolates from HIV seropositive patients presenting with various clinical conditions of candidiasis. Identificaton was carried out as per standard procedures and the coagulase activity was detected by tube coagulase method using three different animal plasmas. Among 179 clinical isolates of Candida tested, high coagulase activity was observed with rabbit plasma (55%) followed by sheep plasma (40.2%). Only C. albicans and C. tropicals showed coagulase activity with Human plasma. http://pubs.sciepub.com/ajmr/2/2/2/ajmr-2-2-2.pdf Candida spp HIV seropositive individuals coagulase activity