@article{ajmr20241222,
author={{Tsague, I. F. Kenfack and Ngouana, T. K. and Dongmo, F. F. Dongho and Kouipou, R. M. Toghueo and Dougue, A. and Yimgang, L. and Abrantes, P. M. D. S. and Sameza, M. L. and Boyom, F. F. and Choudhary, M. I.},
title={Insights into the Prevalence and Resistance Patterns of <i>Candida</i> Species among Diabetic Patients in Cameroon: Potential Paths for Alternative Treatments},
journal={American Journal of Microbiological Research},
volume={12},
number={2},
pages={18--26},
year={2024},
url={https://pubs.sciepub.com/ajmr/12/2/2},
issn={2328-4137},
abstract={The study aimed to fill the knowledge gap regarding the prevalence of <i>Candida</i> infections in diabetic patients and their sensitivity to antifungal drugs. Clinical samples from diabetic patients were collected at Yaound¨¦ Central Hospital and <i>Candida</i> species identified. The ability of these species to form biofilms was assessed, and their susceptibility to usual antifungal drugs, as well as some plant extracts and fractions, was determined. Mycological diagnosis was performed on the collected samples. The <i>Candida</i> species isolated and identified were subjected to the crystal violet assay in a microtiter plate to examine their biofilm-forming abilities. The disk diffusion method was used for susceptibility testing for reference antifungals, while the broth dilution method was employed for plant extracts and fractions. These were obtained from the leaves of <i>Picralima</i><i> </i><i>nitida,</i><i> </i><i>Phragmanthera</i><i> </i><i>capitata</i>, and <i>Spondia</i><i> </i><i>cythereae</i>, and the roots and fruits of <i>Garcinia</i><i> </i><i>Kola</i>. The study found that 43.18% of the 396 collected samples were colonized by various <i>Candida</i> species, including <i>Candida</i><i> </i><i>albicans</i><i>,</i><i> </i><i>Candida</i><i> </i><i>tropicalis,</i><i> </i><i>Candida</i><i> </i><i>parapsilosis,</i><i> </i><i>Candida</i><i> </i><i>glabrata,</i><i> </i><i>Candida</i><i> </i><i>krusei,</i><i> </i><i>Candida</i><i> </i><i>guillermondii,</i><i> </i><i>Candida</i><i> </i><i>lusitaniae,</i> and <i>Candida</i><i> </i><i>ciferrii</i>. Biofilm production was observed in 46.04% of the isolates, with non-<i>albicans</i> <i>Candida</i> species showing a higher rate than <i>Candida</i><i> </i><i>albicans</i>. The isolates exhibited high resistance to nystatin and ketoconazole, dose-dependent sensitivity to miconazole, and sensitivity to econazole and fluconazole. Multidrug resistance was observed in 24.75% of the isolates. While none of the crude extracts showed activity against the tested <i>Candida</i>, six out of 25 fractions (Pnl<SUP>B</SUP>, Pcl<SUP>A</SUP>, Pcl<SUP>W</SUP>, Scl<SUP>A</SUP>, Scl<SUP>W</SUP>, and Gkf<SUP>W</SUP>) displayed fungistatic activities on some strains. These findings offer valuable insights into the prevalence and antifungal resistance patterns of <i>Candida</i> species in diabetic patients in Cameroon. They also suggest the potential for alternative treatments using plant fractions.},
doi={10.12691/ajmr-12-2-2}
publisher={Science and Education Publishing}
}