@article{ajmr20231132,
author={{Nyathi, Makhosazana and Dhlamini, Zephaniah and Ncube, Thembekile},
title={Bioprospecting for Cellulase-Producing Bacteria from Victoria Falls Rainforest Decaying Logs},
journal={American Journal of Microbiological Research},
volume={11},
number={3},
pages={73--78},
year={2023},
url={http://pubs.sciepub.com/ajmr/11/3/2},
issn={2328-4137},
abstract={Bioprospecting for cellulases seeks to find highly active enzymes that are stable, affordable, and can be readily incorporated into industrial processes. Research focus is shifting from fungal to bacterial cellulases due to the latter having higher growth rates, being easy to handle, and being more adaptable to genetic manipulations. In this research, bioprospecting for cellulolytic bacteria was carried out in decaying logs and soils sampled from the Victoria Falls rainforest. A compound sample of decaying logs and soils was inoculated on Mandel's media to isolate cellulase-producing bacteria. Morphological and biochemical analysis of the bacterial isolates was carried out to identify unique isolates showing cellulase production. Two Gram-negative and two Gram-positive isolates were selected and subsequently identified as <i>Acinetobacter sp 9, Enterobacter sp, Bacillus thuringensis,</i> and<i> Bacillus cereus strain HYM88 </i>by 16S rDNA sequencing. Submerged fermentation of the selected cellulase-producing bacteria was carried out for cellulase production. The cellulases were partially characterized to determine their optimum pH and temperature for activity. The optimum cellulase activity from all the bacterial isolates was at pH 5 and 50 ˇăC. The diversity of the cellulases produced was determined. All the bacterial isolates proved to be true cellulolytic bacteria as they produced both exoglucanases and endoglucanases. All the bacterial isolates produced more exoglucanases than endoglucanases by 80 to 90%. The isolated cellulase-producing bacteria are a reflection of Victoria Falls rain forest's potential as an enzyme bioprospecting site which is yet to be tapped.},
doi={10.12691/ajmr-11-3-2}
publisher={Science and Education Publishing}
}