@article{ajmr20231122,
author={{Ambata, Henri Tibo Ambata and Ayong, Mo?se Ntah A and Jiogue, Benissa Mylaure and Dikongue, Jeanne Fabiola Ndondoni and Ndjen, Felecite Nyami and Kamsu, Francine Pamela Noumegna and Youassi, Olivier Youassi and Tchameni, S¨¦verin Nguemezi and Sameza, Modeste Lambert},
title={Antagonistic Effect and Antifungal Activity of Organic Extracts of <i>Trichoderma harzianum </i>and <i>Trichoderma </i><i>asperelloides</i> toward <i>F</i><i>usarium oxysporum elaedis</i>, the Causal Agent of Fusariose Oil Palm},
journal={American Journal of Microbiological Research},
volume={11},
number={2},
pages={40--46},
year={2023},
url={http://pubs.sciepub.com/ajmr/11/2/2},
issn={2328-4137},
abstract={This study aimed to evaluate the antagonistic effet and organic extract of<i> T. harz</i><i>i</i><i>anum </i>and<i> </i><i>T. asperolloide</i>s<b> </b>against <i>Fusarium oxy</i><i>s</i><i>porum elaedis</i>, agent responsible of fusariose of oil palm (<i>Elaeis guineensis Jacq</i>). <i>In vitro,</i> the antagonistic activities were carried out by direct confrontation and antibiosis. The ability of <i>Trichoderma </i>spp to produce hydrolytic enzymes was determined in specific solid media. After fermentation, organic extracts were obtained, phenolic and flavonoid compounds were evaluated and their antifungal activity was done <i>in vitro</i> against mycelial growth of the pathogen. The results obtained show that the both antagonists used have significantly reduced the mycelian growth of the pathogen. In direct cofrontation, the  inhibition of myelian growth was<i> </i>64.58% and 70.8% respectively, for <i>T. harz</i><i>i</i><i>anum</i><i> and T.asperelloides</i>. The non-volatile compounds produced the inhibition of mycelial growth of <i>Fusarium</i> <i>oxysporum</i><i>. </i>The inhibition<i> was </i>75.0% and 71.0%, respectively. The inhibition percentage of volatile compounds was 43.75% and 25.00% respectively. <i>T. harz</i><i>i</i><i>anum</i><i> </i>and <i>T. asperelloides</i> <i> </i>produced respectively<i>, </i>cellulases (62.3mm and 66.3mm), lipases (43mm and 66.7mm), amylases (44.6 mm and 68.3 mm) and proteases (54.6 mm and 52mm). The organic extract of these antagonists¡¯ content phenolic and flavonoids compounds. At 400 ¦Ìg/ml these organic extracts significantly inhibited the mycelial growth of <i>F. oxysporum</i>. The inhibition was 69.29% and 40.69% respectively for crude extract of <i>T. harz</i><i>i</i><i>anum</i> and <i>T.</i><i> </i><i>asperelloides</i>. There were significant and positive correlation between polyphenol and flavonoid content and the inhibition of mycelial growth of the pathogen. These results showed that, <i>T. harz</i><i>i</i><i>anum</i><i> </i>could be used to develop a bioproduct to protect oil palm.},
doi={10.12691/ajmr-11-2-2}
publisher={Science and Education Publishing}
}