eng Science and Education Publishing American Journal of Microbiological Research 2328-4137 2022-09-19 10 2 59 70 10.12691/ajmr-10-2-3 AJMR20221023 article Morabandza Cyr Jonas moressejonas@gmail.com 1 2 Gats¨¦ Elgie Viennechie 2 2 Mboukou Kimbatsa Ir¨¨ne Marie Cecile 2 2 Onyankouang Isaac Samuel 2 2 Ifo Suspence Averti 3 Nguimbi Etienne 3 3 Laboratory of Microbiology, Infectiology and Immunology, ENS-UMNG, B.P 69 Brazzaville Laboratory of Remote Sensing and Forest Ecology, ENS-UMNG, B.P 69 Brazzaville The aims of this study were to characterized the bacteria isolated from the soils of Likouala peat bog area in Congo-Brazzaville. Three (03) composite samples were made from a total of 9 soil samples collected. 1g of soil from each composite sample was cultured on PCA, Mossel and EMB media for counting the total flora. Isolation on Mossel medium and identification of isolates was based on cultural, morphological and biochemical characters by conventional techniques. The method of Petri dishes containing the substrate relating to the search for proteolytic and amylolytic activities was used. The results showed total bacterial loads of 9.50x104; 7.31x104 and 6.39x104 CFU/g on PCA medium; 5.81x104; 5.64x104 and 8.56x103 CFU/g on Mossel medium versus 2.77x102 CFU/g; 5.1x102 and 1.16x102 CFU/g on EMB medium respectively for samples 1, 2 and 3. The respective total anaerobic mesophilic flora of 3.08x104; 2.93x104 and 3.03x103 CFU/g on PCA medium; 2.48x103; 4.83x103 and 3.32x103 CFU/g on Mossel medium for samples 1, 2 and 3. The optical densities of the aerobic isolates were between 0.634 and 1 and the enzyme production showed diameters of translucent halos between 1 to 3.2 cm for the proteolytic enzymes and between 1.2 to 2.7 cm for the amylolytic enzymes. The evolution of enzyme production is a function of time and of the isolate. The surface part of the soils of the Likouala peat bog area is rich in aerobic and anaerobic bacteria. http://pubs.sciepub.com/ajmr/10/2/3/ajmr-10-2-3.pdf bacteria peatland amylase soil protease