eng Science and Education Publishing American Journal of Microbiological Research 2328-4137 2022-06-06 10 2 50 54 10.12691/ajmr-10-2-1 AJMR20221021 article Puneeta Singh Puneeta.singh@lalpathlabs.com 1 Shalabh Malik 1 Vandana Lal 2 Department of Microbiology and Serology, Dr Lal Path Labs, National Reference Laboratory, Delhi, India Dr Lal Path Labs, National Reference Laboratory, Delhi, India Aim: The aim of this study was to assess the prevalence of infection, antimicrobial susceptibility pattern of Stenotrophomonas maltophilia in diverse samples in the Delhi. Materials and Methods: This is a retrospective study conducted over a period of three years, i.e., January 2019 to December 2021. All clinical samples received in the microbiology laboratory at Dr Lal Path Labs, Delhi during the study period were processed using standard microbiological procedures. Antibiotic susceptibility was performed for Levofloxacin, Minocycline and Trimethoprim-sulfamethoxazole by Vitek 2 system (bioMerieux, India) as per CLSI guidelines. Results and Discussion: A total of 9615 non fermenters were isolated, among the non-fermenters, 375 (3.9%) were Stenotrophomonas maltophilia. The most prevalent source of S. maltophilia was largely isolated from blood (34.7%) followed by fluids (20.3%), respiratory (17.8%) and other specimens. This study highlighted potency and the limitation of available agent in the era of antibiotic resistance especially in Delhi, India. Our study describes the distribution and antibiotic resistance of Levofloxacin, Trimethoprim-sulfamethoxazole and Minocycline based on cumulative interpretation and MIC across all age groups. Conclusion: The Stenotrophomonas maltophilia isolates collected in our study had relatively high susceptibility to Minocycline, good susceptibility to Trimethoprim/sulfamethoxazole, but low susceptible to Levofloxacin. Minocycline, could be useful alternative treatment options in Trimethoprim/sulfamethoxazole and Levofloxacin resistant strains. http://pubs.sciepub.com/ajmr/10/2/1/ajmr-10-2-1.pdf Stenotrophomonas maltophilia (S maltophilia) Trimethoprim/sulfamethoxazole (TM/SXT) minocycline Levofloxacin (LVX) non fermenters nosocomial