Science and Education Publishing American Journal of Microbiological Research 1 1 2013 10 09 Modified Protocol for Yeast Identification Using Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry 71 73 EN Janet W. S. Cheng Yit Er Tang Roland Jureen Raymond T.P. Lin Jeanette W.P. Teo Department of Laboratory Medicine, Microbiology Unit, National University Hospital, Singapore, Republic of Singapore AJMR2013142 10.12691/ajmr-1-4-2 2013 08 19 2013 08 26 2013 10 09 Advances in Matrix-assisted Laser Desorption Ionization–time of Flight Mass Spectrometry (MALDI-ToF MS) identification methods have greatly facilitated the rapid identification of yeasts. We validate the use of a shorter on-plate extraction method as well as a lower score cutoff value on the Bruker Biotyper. Overall, when the on-plate extraction method was used together with a score cutoff of > 1.7, a total of 151 of 187 (81%) Candida isolates were correctly identified. At species level, almost 100% of C. krusei and C. albicans isolates were correctly identified. We find the revised protocol to be effective for routine identification of yeasts.