eng Science and Education Publishing American Journal of Microbiological Research 2013-10-09 1 1 71 73 10.12691/ajmr-1-4-2 AJMR2013142 article Janet W. S. Cheng 1 Yit Er Tang 1 Roland Jureen 1 Raymond T.P. Lin 1 Jeanette W.P. Teo Jeanette_Teo@nuhs.edu.sg 1 Department of Laboratory Medicine, Microbiology Unit, National University Hospital, Singapore, Republic of Singapore Advances in Matrix-assisted Laser Desorption Ionization–time of Flight Mass Spectrometry (MALDI-ToF MS) identification methods have greatly facilitated the rapid identification of yeasts. We validate the use of a shorter on-plate extraction method as well as a lower score cutoff value on the Bruker Biotyper. Overall, when the on-plate extraction method was used together with a score cutoff of > 1.7, a total of 151 of 187 (81%) Candida isolates were correctly identified. At species level, almost 100% of C. krusei and C. albicans isolates were correctly identified. We find the revised protocol to be effective for routine identification of yeasts. http://pubs.sciepub.com/ajmr/1/4/2/ajmr-1-4-2.pdf protein extraction Candida identification scores