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Spermogram and Male Fertility: Hospital Experience in Military Avicenna of Marrakech

Zemrani Y*, Taoufik L, Ait Zirri K, Ghoundale O, El Mezouari E, Moutaj R

Department of Parasitology and Mycology, Military Hospital Avicenne, Faculty of Medicine and Pharmacy, Marrakech, Morocco

Abstract: Male fertility is known in the last twenty years. Thus, the 1/3 of the infertility of the couple would be male. The spermogram is an essential tool in the strategy of exploration of infertility in humans. The present study will focus exclusively on the place of occupation spermogram in the appearance of the infertile couple. We wrote this retrospective study spread over 10 results of 929 spermograms performed at the Avicenne Military Hospital in Marrakesh, spermogram volume in ml, pH, sperm count / ml, spermatozoa vitality, total mobility, progressive mobility and morphology. Of the 929 sperm samples, 35% on the normal values and 65% on the normal to less than normal values on the basic parameters, from 65% to today. Found in 5.4% of cases, asthenospermia in 46% of cases, oligospermia in 24.5% and revelation teratospermia in 21.9% of cases. After our study, we are confronted like other countries, the problem of male infertility. The spermogram is the essential exam for the exploration of the male side in an infertile couple to make diagnoses, but also the doctor treating additional examinations.

Keywords: spermogram - male fertility - couple sterility

Received April 11, 2019; Revised May 14, 2019; Accepted June 08, 2019

Citation: Zemrani Y, Taoufik L, Ait Zirri K, Ghoundale O, El Mezouari E, Moutaj R, Spermogram and Male Fertility: Hospital Experience in Military Avicenna of Marrakech. American Journal of Medical Case Reports, 7 (6). 104-108. January 2019. doi:10.12691/ajmcr-7-6-3

1. Introduction

The World Health Organization (WHO) defines the infertility of a couple whose wife is of childbearing age as "the inability to conceive or obtain a pregnancy beyond 12 months of intercourse unprotected regular sex. It is called primary when there has never been a pregnancy and secondary if after one or more pregnancies, the woman does not manage to become pregnant when she wishes.

The woman has long been considered the main culprit of conjugal infertility. However, for decades, the progress of medicine in general and those of reproductive biology in particular have established that the responsibility of the man in the infertility of the couple in 20 to 30% of the cases according to the French epidemiological bases or North American [1]. Infertility is therefore a public health problem and also a serious social problem.

To evaluate male fertility and infertility, the spermogram is the essential test for the exploration of the male side in an infertile couple to make diagnoses, but also to guide the prescriber to further examinations. Our work aims to focus on the place of the spermogram in the exploration of the infertile couple.

2. Patients and Methods

We conducted a 10-year retrospective study (from 10/08/2008 to 31/12/2018) involving 929 spermograms performed at the parasitology-mycology department of the Avicenne military hospital in Marrakech. All subjects were advised to abstain from ejaculation for a period of 3 to 5 days. The period of abstinence from ejaculation was calculated as the time elapsed between current and previous ejaculations, as reported by the subjects. Sperm is collected in our laboratory after urination in a sterile plastic container. Sperm samples were anonymous serial number and incubated in the oven at 37 ° C until analysis. All samples were analyzed within 60 minutes of collection.

The study of the spermogram concerns the following criteria: appearance (color, odor), viscosity, volume in ml, and pH, sperm count / ml, spermatozoa vitality, total mobility, progressive mobility and morphology. Sperm volume was measured after liquefaction of the samples. The sperm count is performed in cells (Malassez or Kova) after homogenization, dilution and immobilization of spermatozoa. The vitality of spermatozoa is obtained after staining of a sample with a vital dye (eosin-nigrosine). Mobility is assessed on a sperm sample between lamella and lamella. Two types of motility were evaluated in this study: total mobility and progressive mobility. The morphological analysis of spermatozoa involves the study of the head, the intermediate piece and the flagella after fixation and staining of a sperm smear. Spermoculture is required to complete the examination.

3. Results and Analyzes

A total of 929 spermograms were included in our study. The mean age of the study population ranged from 24 to 51 years with an average of 35 years. The spermograms requested were indicated in the assessment of primary infertility in 75% of cases and in 25% of cases as secondary. All samples were analyzed within 30 to 60 minutes of collection. The majority of sperm samples were gray or greyish-yellow (98.2%), and liquefaction time was less than 60 minutes in 96% of cases.

The results of spermograms were interpreted according to the criteria of the WHO version 2010 [2] (Table 1) and were as follows: the sperm volume was normal (≥ 1.5 ml) in the majority of cases (94.5 %) with an average of 2.7ml. PH values ranged from 7-10 with normal pH in 74.2% of patients.

Table 1. Reference values of sperm parameters

Figure 1. Distribution of patients according to the spermiological profile

Oligospermia was found in 24.5% of patients while azoospermia occurred in only 5.4% of cases. Total mobility was ≤40% in 29.4% of patients, whereas progressive mobility could not reach the normal threshold (≥32%) in 62.7% of our patients, so asthenospermia estimated on average at 46%. Sperm vitality was ≥ 58% in 42% of patients, which represents a necrosospermia rate of 58%. 23 patients (2.4%) had leukocyte levels above normal (≤ 1 million leukocytes / ml). The normal sperm morphology was in normal values in 88.1% according to WHO criteria, so teratospermia was revealed in 21.9% of cases.

Of the 929 sperm samples evaluated, 35% displayed normal sperm parameters according to the WHO criteria, but 65% had values below the normal threshold in at least one of the sperm parameters, including sperm volume., sperm concentration, total number, vitality, mobility and normal morphology.

4. Discussion

Having a healthy sexuality and motherhood, being a parent, are social necessities. Infertility is defined as the absence of pregnancy after more than 12 months of regular sexual intercourse without contraception. It affects about 15% of couples of childbearing age. Male or mixed causes account for about 50% of infertility [3]. A decrease in semen quality was observed as early as 1980, then confirmed by Meta-analyzes and weighted by taking into account different methodological and environmental factors [4]. In a large recent Danish study, 35.6% of men's infertility etiologies remained unexplained [5]. Recent data from the literature suggest hypotheses about the impact of epigenetics, body mass index (BMI) and lifestyle on reproductive function [6]. These consequences are all the more important since the epigenetic changes in sperm seem to be transmitted to offspring: this is the transgenerational effect. In addition, sperm quality may reflect men's health status [7].

Spermatogenesis is a complex and continuous process in which spermatogonia, diploid stem cells, differentiate into spermatocytes during mitosis and then into spermatocytes II, during meiosis and then into round spermatids. It is only after release of spermatide in the lumen of the tubule that the cell takes the name of "spermatozoid", male gamete haploid. In humans, spermatogenesis begins at puberty and is then continuous throughout life. It is carried out within the epithelium of the testicular seminiferous tubes, in which the Sertoli cells serve as their physical and nourishing support. It lasts 74 days and allows a production of about 200 million sperm per day. A defect of this process can lead to an anomaly of number, mobility, morphology or vitality. Thus each abnormality of spermatogenesis has to be confirmed on 2 samples at 3 month intervals, due in part to the great variability of the spermatic parameters and also to the cycle of spermatogenesis [7].

The analysis of the spermogram should be done according to the time of abstinence. The classic delay of 2 to 7 days is important from the first spermogram [8]. The longer abstinence period can lead to an increase in the volume of the ejaculate, the concentration and the total number of spermatozoa collected. But it can be responsible for a decrease of the spermatic mobility as well as an alteration of the number of typical forms with spermocytogram. Conversely, a shortened abstinence period decreases the count and increases sperm motility [9].

Sometimes noted in the first spermogram, an increase in sperm viscosity may interfere with the determination of sperm motility and concentration. They may be of prostatic origin or secondary to the use of a plastic vial unsuitable for sperm collection [10]. There was no correlation between seminal hyperviscosity, positivity of sperm cultures, leukospermia or the presence of anti-sperm antibodies in sperm [11]. However, an increased rate of failure of in vitro fertilization has been described in the case of seminal hyperviscosity [12]. In an attempt to remedy this, it is possible to predict either a greater fluid intake in the days preceding the collection, or the collection of the ejaculate in a culture medium [10]. Treatment of sperm before intrauterine insemination (IUI) may be considered if there is a clinical problem of hyperviscosity [13].

In our study, the pH in the normal range (pH = 7-8) is 73.8%. The pH should be between 7.2 and 8 and tends to increase with time after ejaculation. Increased pH evokes prostate involvement and decreased pH suggests involvement of vas deferens or seminal vesicles. A low volume sample with a measured pH below 7.0 indicates ejaculatory blockage. A change in pH may also be due to incomplete collection [10,14].

A normal sperm volume (≥1.5ml) was found in 94.5% of our patients. Hypospermia corresponds to an ejaculate volume of less than 1.5 mL on at least two successive spermograms. The causes of hypospermia are numerous and can be divided into two pathophysiological groups: disturbances of the ejaculation reflex resulting in a partial retrograde ejaculation and anatomic (uni- or bilateral deferens agenesis of the vas deferens) and functional (low plasma testosterone) lesions.) glands and seminal ways. In the latter group, mutations in the Cystic Fibrosis Transmembrane Regulator (CFTR) gene, implicated in forms of variable severity of cystic fibrosis, represent a possible cause of hypospermia. The detection of molecular abnormalities of this gene before any hypospermia represents a major stake for the potential progeny of the couple, but also for the patient himself in more or less long term. Treatment of the cause of hypospermia if it does not compromise the subsequent fertility of the patient will be considered as soon as possible [15]. While a volume> 6 ml may be due to too long abstinence or hypersecretion prostatic and vesicular (infection). Remember that the seminal vesicles produce most of the volume of the seminal fluid [2]. However, the increase in seminal volume could not influence sperm parameters, with the exception of the number of sperm [16].

Almost one-third of patients have oligospermia (24.5%), azoospermia is found in 5.4%, while azoospermia requires a complete clinical and paraclinical assessment to understand its origin, particularly obstructive genital tract) or non-obstructive (lack of testicular production). The distinction is based on a bundle of clinical, spermiological, hormonal, ultrasonographic, genetic and histological data. Azoospermia is the main indication for testicular biopsy for therapeutic and diagnostic purposes [17,18].

The percentage of mobility is the parametric parameter of the spermogram: it partly determines the therapeutic orientation. Indeed, only sperm with normal progressive mobility can reach and penetrate the oocyte. Asthénospermie is estimated on average at 46% in our study, which is extremely worrying. Sperm motility is acquired during epididymal transit. Sperm movement is important for migration from the vagina to the fallopian tubes, for penetration into cumulus oophorus and for processes involved in fertilization. Thus, there is an association between sperm mobility and the chances of conceiving naturally [19]. In the interpretation of asthenospermia and apart from cases where abstinence or viscosity are very important, it can be explained either by the presence of flagellary alterations of genetic or congenital origin with viable but immobile spermatozoa, or by necrozoospermia secondary to genital infections, oxidative stress, the presence of antispermatozoids [20], alterations in ATP production [21], toxic exposure [22], abnormalities of epididymal transport of spermatozoa [23], or anejaculation.

The sperm count in sperm is hardly synonymous with its good quality, indeed a sperm may seem to contain enough sperm, but more than half may be dead after staining with eosin or a hypoosmotic test of spermatozoa. Flagellar coil. In fact, 58% of our patients suffer from necrosospermia. This anomaly may be due to too long a period of abstinence or a failure to comply with the recommendations for semen collection, two situations requiring a second spermogram. If confirmed, several pathological mechanisms may be responsible for necrozoospermia. They may be of testicular origin (hyperthyroidism, varicocele, hyperthermia), post-testicular (epididymal necrozoospermia, seminal plasma abnormality, polycystic kidney, post vaso-vasostomy, anti-spermatozoid antibodies) or mixed (infection, toxic, age, injured spinal cord). Treatment is primarily etiological when possible. Fertilization rates are low in cases of necrozoospermia, but in vitro fertilization techniques with intracytoplasmic sperm microinjection improve the chances of conception [24].

Spermocytogram is the examination of sperm morphology after staining slides. Sperm morphology is heterogeneous between individuals and in the same individual, whether fertile or not. If one looks at the data of the literature on more than 30 years of publications, the origin and the impact of many traits or morphological "anomalies" remain unclarified probably because there is a physiological character in the appearance of these anomalies [25].

Although morphological evaluation of the spermatozoon is an integral part of the spermogram and the infertility assessment of the couple, its correlation with fertility remains controversial: most studies show that sperm morphology can be predictive of natural fertility [26,27] intrauterine insemination [28,29], as well as classical in vitro fertilization (IVF) [30,31], but the same is not true of intracytoplasmic sperm injection (ICSI), where the literature review contradictory results. Apart from the gross anomalies of the spermatic heads (macro- or microcephalic, globozoospermia ...) which are associated with fertilization failures and which do not give or very exceptionally pregnancies, it has not been frankly highlighted. Relationship between percent of normal forms and ICSI results: for some authors, fertilization and pregnancy rates are similar for teratospermia and normal sperm [32,33,34,35].

As is the case in our study, the decline in spermogram parameters in different countries around the world remains an undeniable fact [36,37,38,39]. These countries even advocate the installation of the centers just for monitoring the evolution of male fertility. The intervention of other parameters not yet incriminated until our days is discussed more and more. In addition to conventional medico-surgical causes, tobacco, endocrine disruptors and medications would be an important cause among others for the alteration of human sperm [7,40,41,42,43].

5. Conclusion

The steady decline in male fertility over time is proving to be a global scourge and is attracting more and more interest from scientists. Morocco would certainly not be spared as our study shows, although it is conducted on a small scale at the locoregional level and the increasing number of couples using the PMA. Today, biologists will be confronted more than ever with this problem and will have to deal with it thanks to a good knowledge of spermograms and spermocytograms in laboratories and their interpretation, as well as ongoing training on the management of sterile couples from the anamnesis to the prescription of complementary examinations pushed in collaboration with the clinicians to prescribe the treatment adapted to each situation.

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