@article{ajmbr2015361,
author={{Shafik, Noha M. and Mariah, Reham A. and Baghdadi, Hussam and Shafik, Sherine Ragab and El-Ahwal, Loai Mohammed and Sayed, Salah Mohamed El and Omar, Nassar Ayoub Abdellatif},
title={Impact of Nigella Sativa, Omega-3 Fatty Acids and Chromium Picolinate on NF-¦ĘB /leptin-insulin Axis in Obese Subjects with Non-alcoholic Fatty Liver Disease},
journal={American Journal of Medical and Biological Research},
volume={3},
number={6},
pages={139--145},
year={2015},
url={http://pubs.sciepub.com/ajmbr/3/6/1},
issn={2328-4099},
abstract={<b>Background: </b>The global problem of obesity epidemic involves an increased risk of non-alcoholic fatty liver disease (NAFLD) whereby oxidative stress induces fibrogenesis. <b>Objectives: </b>to assess the coordinated impact of obesity management strategy on nuclear factor kappa beta/p65 mRNA expression and biochemical parameters of oxidative stress, glycemic status and adipokines. <b>Subject</b><b>s</b><b> and method</b><b>s</b>: This study was carried out on 60 subjects classified into, 15 normal healthy controls (group ?) and 45 obese subjects who were divided equally into three groups: obese subjects with NAFLD (group ??), obese subjects who received natural product supplements (nigella sativa, green tea andchromium picolinate) (group???),obese subjects who received omega-3 fatty acids (group ?V) andthose who received caloric restricted diet (CRD) and exercise for 6 months. All groups were subjected to measurement of body mass index, waist to hip ratio (WHR), spectrophotometric measurement of serum levels of glucose, malondialdehyde (MDA) and total antioxidant capacity (AOC%), NF-¦ĘB /P65 subunit expression levels by real time-PCR in peripheral bloodmononuclear cells, estimation of levels of insulin, insulin like growth factor-1, leptin, adiponectin, fibronectin and oxidized LDL by enzyme linked immunosorbent assays. <b>Results:</b><b> </b>There was a significant decrease inNF-¦ĘB/p65 mRNA expression in peripheral blood mononuclear cells, reduction in the levels of oxLDL, decreased insulin resistance and decreased leptin resistance which might be linked to hypoadiponectinemia. Levels of (AOC %) were significantly elevated after treatment. This was evident alongside reduction of BMI, WHR and fibrogenic potential in NAFLD. <b>Conclusion: </b>natural product supplements, CRD and exercise ameliorated the fibrogenic and atherogenic consequences of immune-inflammatory and oxidative stress-induced pathological mechanisms associated with obesity.},
doi={10.12691/ajmbr-3-6-1}
publisher={Science and Education Publishing}
}