eng Science and Education Publishing American Journal of Medical and Biological Research 2328-4099 2016-07-12 3 6 139 145 10.12691/ajmbr-3-6-1 AJMBR2015361 article Noha M. Shafik 1 Reham A. Mariah 1 2 Hussam Baghdadi 2 Sherine Ragab Shafik 3 Loai Mohammed El-Ahwal 4 Salah Mohamed El Sayed salahfazara@yahoo.com, drsalahpediatr@yahoo.com 4 5 Nassar Ayoub Abdellatif Omar 6 Departments of Medical Biochemistry, Faculty of Medicine, Tanta University, Egypt Ph.D in Medical Biochemistry, Faculty of Medicine, Tanta University, Egypt Department of Internal Medicine, Faculty of Medicine, Tanta University, Egypt Department of Anatomy, Faculty of Medicine, Sohag University, Egypt Background: The global problem of obesity epidemic involves an increased risk of non-alcoholic fatty liver disease (NAFLD) whereby oxidative stress induces fibrogenesis. Objectives: to assess the coordinated impact of obesity management strategy on nuclear factor kappa beta/p65 mRNA expression and biochemical parameters of oxidative stress, glycemic status and adipokines. Subjects and methods: This study was carried out on 60 subjects classified into, 15 normal healthy controls (group I) and 45 obese subjects who were divided equally into three groups: obese subjects with NAFLD (group II), obese subjects who received natural product supplements (nigella sativa, green tea andchromium picolinate) (groupIII),obese subjects who received omega-3 fatty acids (group IV) andthose who received caloric restricted diet (CRD) and exercise for 6 months. All groups were subjected to measurement of body mass index, waist to hip ratio (WHR), spectrophotometric measurement of serum levels of glucose, malondialdehyde (MDA) and total antioxidant capacity (AOC%), NF-ʦB /P65 subunit expression levels by real time-PCR in peripheral bloodmononuclear cells, estimation of levels of insulin, insulin like growth factor-1, leptin, adiponectin, fibronectin and oxidized LDL by enzyme linked immunosorbent assays. Results: There was a significant decrease inNF-κB/p65 mRNA expression in peripheral blood mononuclear cells, reduction in the levels of oxLDL, decreased insulin resistance and decreased leptin resistance which might be linked to hypoadiponectinemia. Levels of (AOC %) were significantly elevated after treatment. This was evident alongside reduction of BMI, WHR and fibrogenic potential in NAFLD. Conclusion: natural product supplements, CRD and exercise ameliorated the fibrogenic and atherogenic consequences of immune-inflammatory and oxidative stress-induced pathological mechanisms associated with obesity. http://pubs.sciepub.com/ajmbr/3/6/1/ajmbr-3-6-1.pdf non-alcoholic fatty liver disease NF-ʦB/p65 nigella sativa chromiumpicolinate omega-3 fatty acids