eng Science and Education Publishing American Journal of Medical and Biological Research 2328-4099 2015-06-22 3 4 113 116 10.12691/ajmbr-3-4-6 AJMBR2015346 article Jimmy Panelewen 1 Aryono Djuned Pusponegoro 2 Ibrahim Labeda 3 Andi Asadul Islam 3 Mochammad Hatta hattaram@indosat.net.id 4 Department of Surgery, Faculty of Medicine, Sam Ratulagi University, Manado, Indonesia Department of Surgery, Faculty of Medicine, Indonesia University, Jakarta, Indonesia Department of Surgery, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia Molecular Biology and Immunology Laboratory, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia Background: Purpose : To understand the expression of matrix metalloproteinase (MMP)-8 mRNA gene and tissue inhibitors of metalloproteinase (TIMP)-1 in normal peritoneum tissue and intraperitoneal adhesion tissue after the usage of virgin coconut oil (VCO) and hyaluronate Acid-carboxymethylcellulose (HA-CMC). Methods: The research is using an experimental lab rat 'rattus norvegicus strain wistar' which is divided into 3 groups. Laparotomy (pre) is done to gain parietal peritoneum tissue at the lower left quadrant of the abdomen, in order to obtain the expression of mRNA gene MMP-8 and TIMP-1. First group was control group, second group was treated with VCO, while the third group was treated with HA-CMC. After two weeks period, another relaparotomy (post) was held to obtain the adhesion tissue and to run another examination on the MMP-8 mRNA gene expression and TIMP-1. Results: There is a meaningful decrease for the MMP-8 mRNA gene expression in the peritoneum tissue for the control group at the pre and post laparotomy (p<0,001). There is a meaningful over expression of TIMP-1 mRNA gene expression at pre dan post laparatomy of control group (p<0,001). While on the other hand, at the VCO nor HA-CMC group, there is an insignificant increase of MMP-8 mRNA gene expression (p=0,075 and p=0,791) and insignificant decrease of TIMP-1 mRNA gene expression (p=0,015 and p=0,368). Conclusion: The decrease of MMP-8 mRNA gene expression and the increasing of TIMP-1 mRNA gene expression of control group shows a high risk of adhesion incidence. Although it is not statistically meaningful, the result of MMP-8 mRNA and TIMP-1 mRNA gene expression of VCO and HA-CMC indicates that there is a decrease of intraperitoneal adhesion risks, and the nature of VCO anti adhesive compared to HA-CMC. http://pubs.sciepub.com/ajmbr/3/4/6/ajmbr-3-4-6.pdf intraperitoneal adhesion MMP-8 mRNA gene TIMP-1 mRNA gene VCO HA-CMC