@article{ajmbr2015346,
author={{Panelewen, Jimmy and Pusponegoro, Aryono Djuned and Labeda, Ibrahim and Islam, Andi Asadul and Hatta, Mochammad},
title={Analysis of expression of mRNA Matrix Metalloproteinase (MMP)-8 gene and Tissue Inhibitors of Metalloproteinase (MMP)-1 in Intraperitoneal Adhesion after Usage of Hyaluronate acid-Carboxymethylcellulose (HA-CMC) or Virgin Coconut Oil (VCO)},
journal={American Journal of Medical and Biological Research},
volume={3},
number={4},
pages={113--116},
year={2015},
url={http://pubs.sciepub.com/ajmbr/3/4/6},
issn={2328-4099},
abstract={<b>Background: Purpose</b> : To understand the expression of matrix metalloproteinase (MMP)-8 mRNA gene and tissue inhibitors of metalloproteinase (TIMP)-1 in normal peritoneum tissue and intraperitoneal adhesion tissue after the usage of virgin coconut oil (VCO) and hyaluronate Acid-carboxymethylcellulose (HA-CMC). <b>Methods</b>: The research is using an experimental lab rat ¡°<i>rattus</i><i> </i><i>norvegicus strain wistar</i>¡± which is divided into 3 groups. Laparotomy (pre) is done to gain parietal peritoneum tissue at the lower left quadrant of the abdomen, in order to obtain the expression of mRNA gene MMP-8 and TIMP-1. First group was control group, second group was treated with VCO, while the third group was treated with HA-CMC. After two weeks period, another relaparotomy (post) was held to obtain the adhesion tissue and to run another examination on the MMP-8 mRNA gene expression and TIMP-1. <b>Results</b>: There is a meaningful decrease for the MMP-8 mRNA gene expression in the peritoneum tissue for the control group at the pre and post laparotomy (p&lt;0,001). There is a meaningful over expression of TIMP-1 mRNA gene expression at pre dan post laparatomy of control group (p&lt;0,001). While on the other hand, at the VCO nor HA-CMC group, there is an insignificant increase of MMP-8 mRNA gene expression (p=0,075 and p=0,791) and insignificant decrease of TIMP-1 mRNA gene expression (p=0,015 and p=0,368). <b>Conclusion</b>: The decrease of MMP-8 mRNA gene expression and the increasing of TIMP-1 mRNA gene expression of control group shows a high risk of adhesion incidence. Although it is not statistically meaningful, the result of MMP-8 mRNA and TIMP-1 mRNA gene expression of VCO and HA-CMC indicates that there is a decrease of intraperitoneal adhesion risks, and the nature of VCO anti adhesive compared to HA-CMC.},
doi={10.12691/ajmbr-3-4-6}
publisher={Science and Education Publishing}
}