@article{ajmbr2015323,
author={{Alharth, Ali S. and El-Daly, Sherien M.},
title={Effects of Tenascin-C (TNC) Knockdown on Global Genes Expression},
journal={American Journal of Medical and Biological Research},
volume={3},
number={2},
pages={62--67},
year={2015},
url={http://pubs.sciepub.com/ajmbr/3/2/3},
issn={2328-4099},
abstract={The extracellular matrix protein tenascin-C (TNC) is up-regulated in many cancers including breast cancer. TNC is associated with tumour progression and poor prognosis. The aim of this study was to investigate the effects of TNC knockdown on global gene expression in TNC expressing invasive breast cancer cell lines. Breast cancer cell lines (MDA-MB-231 and MDA-MB-436) were transfected with small interfering RNA (siRNAs) targeting total TNC. cDNA microarray was used to analyse the effects of TNC knockdown on global gene expression at the mRNA level. Microarray analysis following total TNC knockdown revealed significant changes in gene expression: <i>CREBL2, YWHAE </i>and<i> RRAS2 </i>showed down regulation and <i>QKI </i>was specifically up-regulated. In conclusion, the silencing of TNC expression caused significant alteration on global genes expression associated with tumour progression.},
doi={10.12691/ajmbr-3-2-3}
publisher={Science and Education Publishing}
}