@article{ajmbr20261422,
author={{Appolinaire, Tedanb¨¦ Pakeu and Doloum, Gomoung and Mbaindogoum, Koundambaye and Lamerdje, Mon¨¦ Lakass¨¦ and Kakess¨¦, Maguirgu¨¦ and Hassane, Mansour and Dassidi, Nid¨¦ou},
title={Phytochemical screening and antioxidant activity of extracts from <i>Bauhinia rufescens</i> Lam. and <i>Physalis angulata</i> Linn., used in the treatment of ascariasis in Chad},
journal={American Journal of Medical and Biological Research},
volume={14},
number={2},
pages={30--38},
year={2026},
url={https://pubs.sciepub.com/ajmbr/14/2/2},
issn={2328-4099},
abstract={Ascariasis is an infection that primarily affects children living in low-income areas, particularly due to a lack of sanitation facilities, high medication costs, and sociocultural practices. The objective of this study was to identify the phytochemical families and evaluate the in vitro antioxidant activity of <i>Physalis angulata</i> and <i>Bauhinia rufescens</i>. The phytochemical analysis revealed a wide variety of bioactive compounds (polyphenols, alkaloids, flavonoids, tannins, terpenoids, and saponins). The total polyphenol content was determined using the Folin-Ciocalteu method, while antioxidant activity was assessed using the DPPH radical scavenging assay. Inhibitory activity was determined using a colorimetric method. The highest levels were observed for total polyphenols, at 76.51 ¡À 0.36 mgEqAG/gDM (milligrams of gallic acid equivalent per gram of dry matter) in the methanol extract, followed by alkaloids, 68.35 ¡À 0.41 mgEqQui/gDM (milligrams of quinine per gram of dry matter) for <i>B. rufescens</i> leaves, and 72.52 ¡À 0.22 mgEqAG/gDM and 68.35 ¡À 0.41 mgEqQui/gDM, for total polyphenols and alkaloids, respectively, in <i>P. angulata</i>. The leaves of B. rufescens exhibited DPPH radical scavenging activity, indicating concentration-dependent antioxidant activity, with inhibition rates of 79.65% for the methanolic extract (ME), 62.05% for the aqueous extract (AE), and 32.89% for the hexane extract (HE). A dose-dependent effect was observed for BHT, with concentrations ranging from 50 to 200 ¦Ìg/mL in the ABTS¡¤+ assay. Butylhydroxytoluene (BHT) scavenged 90.25% of hydroxyl radicals at a maximum concentration of 250 ¦Ìg/mL. Furthermore, hydroxyl radical scavenging activity (HRSA) testing showed that the methanolic extract was most effective at reducing ferric ions to ferrous ions. This study highlights the potential of oxidative activity as a natural source of bioactive molecules that may contribute to the treatment of parasitic infections.},
doi={10.12691/ajmbr-14-2-2}
publisher={Science and Education Publishing}
}