eng Science and Education Publishing American Journal of Infectious Diseases and Microbiology 2328-4064 2021-11-04 9 4 136 141 10.12691/ajidm-9-4-5 AJIDM2021945 article Amal Almedaini Amalalmedaini@gmail.com 1 Anfal Al Bujayr 1 Khalid H. Alanazi 1 General Directorate of Infection Prevention and Control (GDIPC), Ministry of Health (MOH), PO Box: 11176, Riyadh, Kingdom of Saudi Arabia OBJECTIVES: To investigate the knowledge, attitude, and practice regarding sterilization among CSSD staff working in Saudi MOH hospitals. STUDY DESIGN: cross-sectional study. METHODOLOGY: This study carried over 5 months (Dec 2020 - Apr 2021) targeted 371 CSSD working staff who are directly involved in reprocessing surgical instruments and medical equipment in the Saudi MOH Hospitals in all regions. Data collected by self-administered questionnaire consisting of personal information, work characteristics and the Knowledge Attitude and practice scale (KAP). RESULTS: MOST CSSD staff, either Specialized in CSSD or not, have sufficient knowledge regarding sterilization techniques. A positive correlation exists between knowledge-attitude, knowledge-practice and attitude-practice. It has been found some significate correlations between two of the study variables and the knowledge, attitude, and practice of the study population. As expected there was a relationship between Specialty in sterilization and knowledge, but surprisingly, there was no relationship with practice and attitude. Also, there was a relationship between practice, attitude, and years of experience, But no relationship with knowledge. CONCLUSION: CSSD staff in Saudi MOH hospitals show very good knowledge and are having a high positive attitude towards the use of sterilization techniques, and are efficiently practicing the sterilization techniques. http://pubs.sciepub.com/ajidm/9/4/5/ajidm-9-4-5.pdf Central Sterile Supply Department (CSSD) Sterilization Healthcare-Associated Infection (HAI) Knowledge Attitude practice (KAP) Healthcare workers (HCWs)