eng Science and Education Publishing American Journal of Infectious Diseases and Microbiology 2328-4064 2018-10-16 6 2 62 65 10.12691/ajidm-6-2-5 AJIDM2018625 article Marwan Y. Al-Maqtoofi marwan.almaqtoofi@outlook.com 1 Department of Biology, College of Sciences, University of Basrah, 61004, Iraq Opportunistic fungi belonging to the Fusarium solani have become increasingly recognised as life-threatening pathogens causing keratitis and disseminated fusariosis among both healthy individuals and patients with haematological malignancies. These infections are associated with biofilm formation on different biotic and abiotic surfaces. Considering, a biofilm is a virulence factor for causing infections, the aim of this study optimising and illustrating a simple, cost-effective and highly reproducible 96 well microtitre-based method for F. solani biofilm formation via using crystal violet stain. The results revealed that the possibility of using either 570nm or 595nm as a wavelength for quantifying fungal biofilm formation. The best time for crystal violet de-staining was 10 min of incubation. This model can be used in-vitro to quantify and understand the virulence factor of fungal biofilm during infections, and for antifungal susceptibility testing. http://pubs.sciepub.com/ajidm/6/2/5/ajidm-6-2-5.pdf Biofilm formation Fusarium solani fungi crystal violet 96 well microtitre plates