@article{ajidm2018625,
author={Al-Maqtoofi, Marwan Y.},
title={Optimising a <i>Fusarium</i> <i>solani</i> Biofilm Formation Protocol in Vitro},
journal={American Journal of Infectious Diseases and Microbiology},
volume={6},
number={2},
pages={62--65},
year={2018},
url={http://pubs.sciepub.com/ajidm/6/2/5},
issn={2328-4064},
abstract={Opportunistic fungi belonging to the <i>Fusarium</i> <i>solani</i> have become increasingly recognised as life-threatening pathogens causing keratitis and disseminated fusariosis among both healthy individuals and patients with haematological malignancies. These infections are associated with biofilm formation on different biotic and abiotic surfaces. Considering, a biofilm is a virulence factor for causing infections, the aim of this study optimising and illustrating a simple, cost-effective and highly reproducible 96 well microtitre-based method for <i>F.</i> <i>solani</i> biofilm formation via using crystal violet stain. The results revealed that the possibility of using either 570nm or 595nm as a wavelength for quantifying fungal biofilm formation. The best time for crystal violet de-staining was 10 min of incubation. This model can be used in-vitro to quantify and understand the virulence factor of fungal biofilm during infections, and for antifungal susceptibility testing.},
doi={10.12691/ajidm-6-2-5}
publisher={Science and Education Publishing}
}