eng Science and Education Publishing American Journal of Infectious Diseases and Microbiology 2328-4064 2017-11-02 5 4 132 136 10.12691/ajidm-5-4-2 AJIDM2017542 article Maha I. Hamed mahohzero@gmail.com 1 Terri L. McCalla 2 Wendy M. Townsend 3 Mohamed N. Seleem 4 Animal Medicine Department, Faculty of Veterinary Medicine- Assiut University, Assiut, Egypt Animal Eye Care LLC, Bellingham WA, USA Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Purdue University, West Lafayette, Indiana, USA Department of Comparative Pathobiology, Collage of Veterinary Medicine, Purdue University, West Lafayette, Indiana, USA Two dog cases with history of recurrent bacterial conjunctivitis were submitted to Purdue University for bacterial culture and sensitivity testing. The bacterial cultures were also tested for their ability to form a biofilm. Biofilm forming Staphylococcus pseudintermedius was isolated from the two cases and identified by Vitek2 and PCR analysis. The bacterial antibiotic sensitivity testing revealed that Isolate one was susceptible to ticarcillin, ceftiofur, cefazolin, moxifloxacin, amikacin and rifampicin with MICs ranging 0.03125-4 μg/ml. Isolate two was susceptible to ceftiofur, gatifloxacin, cefazolin, moxifloxacin, tetracycline, doxycycline and rifampicin with MICs ranging <0.0625-4 μg/ml. Meanwhile, the biofilm antibiotic sensitivity testing revealed that Isolate one biofilm was resistant to all antibiotics except for ceftiofur and moxifloxacin with MICs <0.25 and 8 ̦g/ml respectively. Isolate two biofilm was resistant to all antibiotics except for tetracycline, amikacin, doxycycline, chloramphenicol and rifampicin with MICs ranged from <0.125 to 8 ̦g/ml. Case one was resolved with topical cefazolin therapy. Meanwhile case two showed complete recovery after treated with topical levofloxacin plus oral doxycycline. http://pubs.sciepub.com/ajidm/5/4/2/ajidm-5-4-2.pdf Staph pseudintermedius biofilm dogs ophthalmic lesions