@article{ajidm2016444,
author={{Ahmed, Sylvia O. and Hamed, Maha I. and Yones, Doaa A.},
title={Molecular and Conventional Detection of Zoonotic<i> Giardia </i>and <i>Cryptosporidium </i>in Children and Calves in Upper Egypt},
journal={American Journal of Infectious Diseases and Microbiology},
volume={4},
number={4},
pages={91--94},
year={2016},
url={http://pubs.sciepub.com/ajidm/4/4/4},
issn={2328-4064},
abstract={<i>Giardia</i> and <i>Cryptosporidium </i>are wide spread pathogens of human and many species of mammals. This study aimed to investigate the potential direct transmission of <i>Giardia</i> and <i>Cryptosporidium</i> between cattle calves and the surrounding living children and to improve the knowledge of zoonotic Giardiasis and cryptosporidiosis situation in Assiut Governorate, Egypt. Faecal samples from 70 diarrheic children (above 5 years) and 62 diarrheic calves (1-3 months) were collected from the same villages at Assiut Governorate, Egypt. Samples were subjected to conventional microscopic examination for <i>Giardia</i> and<i> Cryptosporidium. </i>Positive samples were subjected to molecular identification. By conventional microscopic examination,<i> Giardia </i>cysts were detected in 27 out of 70 (38.57%) childĄ¯s stool samples, while <i>Cryptosporidium </i>oocyst were detected in 12 samples (17.14%). Mixed infection of<i> Giardia </i>and<i> Cryptosporidium</i> were detected in all<i> Cryptosporidium </i>positive samples. In children, 81.48% of <i>Giardia</i> samples were <i>G. intestinalis</i> assemblage B meanwhile, all samples were negative by PCR for <i>C. parvum.</i> In calves 29.03% of faecal samples were positive for <i>Giardia </i>by microscopic examination, while only 12.90% of those samples were positive for <i>Cryptosporidium </i>oocysts. 21 out of 26 (80.76%)<i> Giardia</i> positive samples were positive to <i>G. intestinalis </i>assemblage B and 22 out of 26<i> Cryptosporidium </i>positive samples (84.61%) were positive for <i>C. parvum. </i>High prevalence of <i>G. intestinalis </i>assemblage B infection detected among children was significantly associated with contact with calves explaining the existence of its zoonotic transmission<i> </i>and more studies are needed to investigate the zoonotic potential of <i>Cryptosporidium</i> in the study area.},
doi={10.12691/ajidm-4-4-4}
publisher={Science and Education Publishing}
}