eng Science and Education Publishing American Journal of Infectious Diseases and Microbiology 2328-4064 2015-11-18 3 5 141 143 10.12691/ajidm-3-5-1 AJIDM2015351 article Meena Mishra 1 Arvind Kurhade 2 Yagnesh Thakar 3 Geeta Kurhade 4 Angel Justiz Vaillant Angel.Vaillant@sta.uwi.edu 5 Sudhir Lakhdive 6 Tejaswini H Joshi 7 Sehlule Vuma 7 Department of Microbiology, Government Medical College and Hospital, Nagpur, India Pathology and Microbiology Unit, Department of Para, Clinical sciences, University of West Indies, St. Augustine, Trinidad and Tobago Consultant Microbiologist, Nagpur, India Physiology Unit, Department of Preclinical Sciences, University of West Indies, St. Augustine, Trinidad and Tobago Pathology and Microbiology Unit, Department of Para Clinical sciences, UWI, St. Augustine, Trinidad and Tobago Professor of Surgery, Great Eastern Medical School, Srikakulum, India Post Graduate Diploma Program, Defense Services Staff College, Madras University, Wellington, India The aim of this research was to carry out a bacteriological study of Vibrio cholerae in Central India. Cholera is an important public health problem in India and it continues to be a major concern as it is an important cause of morbidity mortality. A total of 44 strains of V. cholerae were isolated from 150 stool samples received from patients with acute diarrhea. All samples were plated onto different bacteriological media. Biotyping was performed as per the standard procedures. Confirmation of the strains was done by seroagglutination using Polyvalent O1, monospecific Ogawa and Inaba antisera. Antibiotic susceptibility testing was performed by Kirby Bauer's disk diffusion method. The majority of the isolates belonged to type 27 (70.45%, i.e. 31 isolates). All Isolates were susceptible to tetracycline, norfloxacin and were relatively susceptible to gentamicin (95%) and chloramphenicol (95%). Continued monitoring, surveillance of all outbreaks and notification to relevant authorities are of utmost importance in the fight against cholera. In addition, the molecular subtyping was essential to improve the tracing of the sources of the outbreak. http://pubs.sciepub.com/ajidm/3/5/1/ajidm-3-5-1.pdf Vibrio cholerae bacteria bacteriological media Antibiotic susceptibility India