@article{ajidm2015334,
author={{Burki, Zeba Gul and Hassan, Mukhtiar and Naseer, Faiza and Ahmad, Sohail and Burki, Samiullah and Saleem, Mohammad and Nazish, Attiya and -ur-Rehman, Shafiq},
title={Isolation and Characterization of Microbial Contamination from Computer Accessories used in Different Department of Hazara University and Diagnostic Laboratories of District Mansehra, Pakistan},
journal={American Journal of Infectious Diseases and Microbiology},
volume={3},
number={3},
pages={112--124},
year={2015},
url={http://pubs.sciepub.com/ajidm/3/3/4},
issn={2328-4064},
abstract={150 samples were collected from computer accessories used in Hazara University and different diagnostic laboratories of Mansehra, examined for the total bacterial count and maximum growths were observed. Samples were analyzed for further identification of micro-organisms such as <i>E.coli</i>, <i>Klebsiella</i>,<i> S</i><i>taph. aureus</i> and<i> Staph. </i><i>epidermidis</i><i>.</i> These organisms were detected in the percentage of 46.66 of <i>E. </i><i>coli</i>, 20% of <i>Klebsiella</i>, 16.66% of <i>S.</i><i> </i><i>aureus</i> &amp; 16.66% of <i>S.</i><i> Epidermidis </i>and<i> </i>identified on selective media, i.e. EMB agar and Mannitol salt agar. Furthermore, biochemical tests, including IMVIC Test, Catalase Test and Coagulase Tests were performed to confirm the presence of micro-organisms and their susceptibility also checked against different standard antibiotics and their zone of inhibitions were measured and noticed. <i>E.coli</i><i> </i>showed maximum resistance of<i> </i>97.36<i>%</i> against Erythromycin<i>,</i><i> </i><i>Klebsiella</i> showed against Amoxil + Clavolunic acid about 83.83%, <i>Staph </i><i>aureus</i> showed against Erythromycin about 64.64% and <i>Staph. e</i><i>pidermidis</i><i> </i>resistance was 90.9% against Erythromycin and Gentamycin. <i>E.coli</i> and <i>Klebsiell</i><i>a </i>showed maximum sensitivity for Meropenem 67.22%<i> </i>and<i> </i>72.72% respectively while <i>S</i><i>taph. aureus</i> and<i> Staph. </i><i>epidermidis</i><i> </i>maximum sensitivity for Vancomycin about 82.82% and 72.72% respectively. These results indicate that the computer accessories might act as environmental vehicles for the transmission of potentially pathogenic bacteria in our surroundings and also indicate the need for increasing awareness among computer users on cleaning of such surfaces or disinfection and adequate hand-washing hygiene.},
doi={10.12691/ajidm-3-3-4}
publisher={Science and Education Publishing}
}