@article{ajidm2013131,
author={{Campanha, Nara Hellen and Jorge, Janaina Habib and Giampaolo, Eunice Teresinha and Oliveira, Caio Sergio Botta Martins de and NordiDovigo, L¨ªvia and Maia, Danielle Cardoso G. and Pavarina, Ana Cl¨¢udia},
title={Cell Membrane Integrity of Candida Albicans after Different Protocols of Microwave Irradiation},
journal={American Journal of Infectious Diseases and Microbiology},
volume={1},
number={3},
pages={38--45},
year={2013},
url={http://pubs.sciepub.com/ajidm/1/3/1},
abstract={<b>Purpose:</b> To evaluate the ability of low time microwaveexposureto inactivate and damage cell membrane integrity of <i>C. </i><i>albicans</i>. <b>Materials and Methods:</b> Two 200ml<i> C. albicans</i> suspensions were obtained. Sterile dentures were placed in a beaker containing Experimental (ES) or Control suspensions (CS). ES was microwaved at 650 W for 1, 2, 3, 4 or 5 min. Suspensions were optically counted using Methylene blue dye as indicative of membrane-damaged cells; spread on Agar Sabouraud dextrose (ASD) for viability assay; or spectrophotometrically measured at 550nm. Cell-free solutions were submitted to content analyses of protein (Bradford and Pyrogallol red methods); Ca<SUP>++</SUP> (Cresolphthalein Complexone method); DNA (spectrophotometer measurements at 260nm) and K<SUP>+</SUP> (selective electrode technique). Data were analyzed by Student-<i>t</i> test and linear regression (¦Á=0.05). In addition, flowcytometry analysis of <i>Candida </i>cells in suspensionwas performed using propidium iodide<b>.</b><b> </b><b>Results:</b><b> </b>All ES cells demonstrated cell membrane damage at 3, 4 and 5 min,viable cells were nonexistent at 3, 4 and 5 min ES ASD plates and optical density of ES and CS was not significantly differentfor all exposition times. ES cells released highcontents of protein, K<SUP>+</SUP>, Ca<SUP>++</SUP> and DNA after 2 min exposition when compared to that of the CSs. Similar results were observed with flow cytometry analysiswith regard to the periodsof microwave exposure. <b>Conclusions:</b> Microwave irradiation inactivated <i>C. albicans</i>after 3min and damaged cell membrane integrity after 2 min exposition.},
doi={10.12691/ajidm-1-3-1}
publisher={Science and Education Publishing}
}