eng Science and Education Publishing American Journal of Food Science and Technology 2333-4835 2018-01-18 6 1 7 11 10.12691/ajfst-6-1-2 AJFST2018612 article Patience C. Obinna-Echem chisanupat@yahoo.com, patience.obinna-echem@ust.edu.ng 1 School of Biological Sciences, Faculty of Science and Environment, Plymouth University, Plymouth, United Kingdom This study investigated the ability of two strains of Lactobacillus plantarum isolated from akamu a Nigerian fermented maize food to tolerate acid and bile condition. Auto-aggregation and co-aggregation with pathogens: Escherichia coli NCTC 11560 and Salmonella Enteritidis NCTC 5188 were also investigated. This was aimed at establishing preliminary probiotic potentials of these none intestinal L. plantarum isolates. Viability at pH 2 was significantly (p≤0.05) reduced from ≥8.26±0.05 to ≤4.94±0.49 Log10 CFU/mL after 3 h. Subsequent incubation in 0.3% ox gall bile media after 6 h enhanced growth to 5.73±0.13 and 7.93±0.12 Log10 CFU/mL for NGL5 and NGL7. The L. plantarum strains auto-aggregated but had no co-aggregation with the pathogens. After 5 h auto-aggregation at 37°C (>25%) was significantly (p≤0.05) greater than auto-aggregation at 22 - 24°C (<14%). The L. plantarum strains possessed abilities to survive passage through the GIT and auto-aggregated significantly at body temperature. This serves as a baseline data for further studies especially isolates that are not of intestinal origin. http://pubs.sciepub.com/ajfst/6/1/2/ajfst-6-1-2.pdf Lactobacillus plantarum akamu acid and tolerance auto-aggregation co-aggregation