eng Science and Education Publishing American Journal of Clinical Medicine Research 2328-403X 2020-02-20 8 1 5 12 10.12691/ajcmr-8-1-2 AJCMR2020812 article Hany Salah Mahmoud 1 2 Rehab A. Eltahlawi 3 4 Osama Alhadramy 5 Tamer M. Soliman 6 Yasser Mohamed FathyEl-Kot 7 Nassar Ayoub Abdellatif Omar 8 9 Sayed Mostafa El Sayed 10 11 Hesham I. Abdallah 11 11 Momen El-shazley 12 13 Amal Nor Edeen Ahmad Allithy 14 15 Noha El-Dabie 15 Mohamed Abdel-Haleem 16 Amr El-Dardear 17 Shaima Mohamed Abdelfattah Hassan 18 Manal Mohamed Helmy 19 Hassan El-Alaf 20 Hussam Baghdadi 21 Samer El-Sawy 22 Salah Mohamed El Sayed salahfazara@yahoo.com, drsalahpediatr@yahoo.com 22 22 22 Prophetic Medicine Course and research, Taibah College of Medicine, Taibah University, Al-Madinah Al-Munawwarah, Saudi Arabia. Department of Microbiology and Immunology, Zagazig University, Faculty of Medicine, Zagazig, Egypt Division of Cardiology, Department of Medicine, Taibah College of Medicine, Taibah University, Al-Madinah Al-Munawwarah, Saudi Arabia. Department of Clinical Pathology, Sohag Faculty of Medicine, Sohag University, Egypt Consultant of Family Medicinein Directorate of Health(Al-Madinah Al-Munawwarah, Saudi Arabia) and Atlas Medical Center, Ministry of Health, Cairo, Egypt. Department of Anatomy, Faculty of Medicine, Sohag University, Sohag, Egypt 0Anatomy and Embryology Department, Faculty of Medicine, Taibah University, Saudi Arabia 2Department of Medicine, Taibah College of Medicine, Taibah University, Al-Madinah Al-Munawwarah, Saudi Arabia 4Department of Pathology, Al Rayyan Medical Colleges, Al-Madinah, Saudi Arabia 6Department of Ear, Nose and Throat, College of Medicine, Taibah University, Saudi Arabia 7Department of Pediatrics, College of Medicine, Taibah University, Saudi Arabia 8Department of Histology and Cell Biology, Faculty of Medicine, Menoufia University, Egypt 9Consultant of Pediatric Cardiology, Department of Pediatrics, Maternity and children Hospital (Hail, Saudi Arabia) and Sohag Teaching Hospital (Sohag, Egypt) and Al Rayyan Medical Colleges (Al-Madinah, Saudi Arabia). 0Department of Medical Physiology, Sohag Faculty of Medicine, Sohag University, Egypt 1Department of Clinical Biochemistry and Molecular Medicine, Taibah Faculty of Medicine, Taibah University,Al-Madinah Al-Munawwarah, Saudi Arabia. 2Department of Medical Biochemistry, Sohag Faculty of Medicine, Sohag University, Egypt. Zamzam water is the most beloved potable water for Muslims worldwide. It originates from Zamzam well near Al-Haram mosque in Makkah, Saudi Arabia. The British Broadcasting Corporation (BBC) raised many health concerns regarding the high nitrate and arsenic contents in Zamzam water that warrants further research investigations. In this study, we investigated the presence of bacterial contamination in Zamzam water. We also examined the effects of regular exclusive Zamzam water consumption for three consecutive months on the liver and kidney functions in experimental animals. Ethical committee approval was taken for performing the study. Eighteen white albino mice were divided into three experimental groups (six mice per group): tap water group, distilled water group and Zamzam water group. All mice were sacrificed by the end of the scheduled study duration. Our data confirmed that Zamzam water is pathogen-free. Our data revealed that Zamzam water consumption for three consecutive months was quite safe for the general health, kidney function tests (serum urea and creatinine) and liver function tests (serum ALT, serum AST, serum albumin and serum globulin). All serum parameters were in their normal ranges, which were comparable to mice exclusively drinking tap water and distilled water. Serum albumin was significantly higher (p<0.001) in animals drinking Zamzam water and tap water than those drinking distilled water. Zamzam water consumption for prolonged periods was quite safe with no signs of acute or chronic arsenic toxicity and resulted in normal kidney histological structure. Normal serum AST confirms that Zamzam water is cardioprotective. In other words, BBC concerns regarding high arsenic and nitrate contents may be acceptable in tap water or other types of drinking water apart from Zamzam water. This is because Zamzam water is unique in its antioxidant components that abrogate any oxidative arsenic effects and confers a lot of therapeutic benefits owing to both arsenic and the antioxidants. In conclusion, Zamzam water is cardio-protective, tissue-protective and is promising as future therapeutic water for many ailments. Zamzam water is devoid of any bacteriological contamination. Nitrates in Zamzam water is an original constituent and not due to contamination. Same thing applies to arsenic. Zamzam water is also pathogen-free and is cardioprotective. http://pubs.sciepub.com/ajcmr/8/1/2/ajcmr-8-1-2.pdf Zamzam water kidney functions liver functions AST histology BBC concerns