Science and Education Publishing American Journal of Clinical Medicine Research 2328-403X 3 3 2015 06 24 Evaluation of Alpha (α) and Beta (β) Haemolysin Antibodies Incidence among Blood Group ‘O’ Donors in ATBUTH Bauchi- Nigeria 42 44 EN Obisesan OA Ogundeko TO Department of Pharmacology and Therapeutics, College of Medicine and Health Sciences, Bingham University, Jos, Nigeria Iheanacho CU Abdulrazak T Idyu VC Idyu II Isa AH AJCMR2015332 10.12691/ajcmr-3-3-2 2015 05 20 2015 06 08 2015 06 24 Background: Antibodies with haemolytic properties are common within the ABO system. These lytic antibodies are immunoglobulin G (IgG) and in high titres cause haemolysis during blood transfusion. Information on Immunoglobulin types and concentration of ABO haemolysins in Nigerian population is not readily available especially in Northern Nigeria. Methods: Serum samples from 200 males and 25 females O group blood donors were screened for A and B haemolysins. Forty two positive samples (38 males and 4 females) were treated with dithiothretiol (DTT) for characterization of IgG class. Antibody titre was compared with grade of haemolysis. Results: Out of 50 positive samples 42 highly haemolytic serum samples had IgG titres of > or = 64 after treatment with DTT. There was 18.6% occurrence of α and or β haemolysins in blood group O donors also with average titre values ofα-haemolysins (68.2) and β-haemolysins (67).Conclusion: Results showsclear indication of high incidence ofα and β haemolysins among the healthy blood group O donors hence, Haemolysin test was found to be a useful screening test to identify group O donors with high levels of IgG anti A and/or anti B as far as safe blood transfusion is concerned. Aim: The study was determined the incidence of α and β haemolysins in healthy blood group O donors in the Blood Bank Unit of ATBUTH Bauchi.