eng Science and Education Publishing American Journal of Clinical Medicine Research 2328-403X 2015-06-24 3 3 42 44 10.12691/ajcmr-3-3-2 AJCMR2015332 article Obisesan OA 1 Ogundeko TO tim_ogundeko@yahoo.com 2 Iheanacho CU 2 Abdulrazak T 3 Idyu VC 4 Idyu II 5 Isa AH 6 Department of Haematology, Jos University Teaching Hospital, Nigeria Department of Pharmacology and Therapeutics, College of Medicine and Health Sciences, Bingham University, Jos, Nigeria Department of Haematology, AbubakarTafawaBalewa University Teaching Hospital, Bauchi, Nigeria Department of Clinical Pharmacy, Faculty of Pharmaceutical Sciences, University of Jos, Nigeria Department of Pharmacology, Faculty of Pharmaceutical Sciences, University of Jos, Nigeria Department of Haematology, College of Medicine and Health Sciences, Bingham University, Jos, Nigeria Background: Antibodies with haemolytic properties are common within the ABO system. These lytic antibodies are immunoglobulin G (IgG) and in high titres cause haemolysis during blood transfusion. Information on Immunoglobulin types and concentration of ABO haemolysins in Nigerian population is not readily available especially in Northern Nigeria. Methods: Serum samples from 200 males and 25 females O group blood donors were screened for A and B haemolysins. Forty two positive samples (38 males and 4 females) were treated with dithiothretiol (DTT) for characterization of IgG class. Antibody titre was compared with grade of haemolysis. Results: Out of 50 positive samples 42 highly haemolytic serum samples had IgG titres of > or = 64 after treatment with DTT. There was 18.6% occurrence of α and or β haemolysins in blood group O donors also with average titre values ofα-haemolysins (68.2) and β-haemolysins (67).Conclusion: Results showsclear indication of high incidence of α and or β haemolysins among the healthy blood group O donors hence, Haemolysin test was found to be a useful screening test to identify group O donors with high levels of IgG anti A and/or anti B as far as safe blood transfusion is concerned. Aim: The study was determined the incidence of α and β haemolysins in healthy blood group O donors in the Blood Bank Unit of ATBUTH Bauchi. http://pubs.sciepub.com/ajcmr/3/3/2/ajcmr-3-3-2.pdf alpha (α) and beta (β) haemolysin group “O” gender prevalence Bauchi-Nigeria