@article{ajbr2016431,
author={{Badawi, Marwan Mustafa and Osman, Marwa Mohamed and Alla, Afra AbdElhamid Fadl and Ahmedani, Ammar Mohammed and Abdalla, Mohamed hamed and Gasemelseed, Mosab Mohamed and Elsayed, Ahmed Abubakar and Salih, Mohamed Ahmed},
title={Highly Conserved Epitopes of ZIKA Envelope Glycoprotein May Act as a Novel Peptide Vaccine with High Coverage: Immunoinformatics Approach},
journal={American Journal of Biomedical Research},
volume={4},
number={3},
pages={46--60},
year={2016},
url={http://pubs.sciepub.com/ajbr/4/3/1},
issn={2328-3955},
abstract={Zika virus (ZIKV) is positive sense single stranded RNA of Flavivirus genus belonging to the Flaviviridae family. It has neither drug nor protective vaccine, and considered to be in relatedness to neurological abnormalities such as Guillain Barre Syndrome and microcephaly of neonates. The aim of this study is to analyze envelope glycoprotein E of all Zika strains using <i>in silico</i> approaches looking for conservancy, which is further studied to predict all potential epitopes that can be used after <i>in vitro</i> and <i>in vivo</i> confirmation as a therapeutic peptide vaccine. A total of 50 Zikavirusvariants¡¯ (include 12 from South America) polyproteins retrieved from NCBI database were aligned, and the conserved regions of Envelope Glycoprotein-E were selected for epitopes prediction. IEDB analysis resource was used to predict B and T cell epitopes and to calculate the population coverage. Epitopes with high scores in both B cell and T cell epitopes predicting tools were suggested. Three epitopes were proposed for international therapeutic peptide vaccine for B cell (<b>AQDKP</b>, <b>TPNSPRAE</b> and <b>TPHWNNK</b>) and two other epitopes designed especially for South America strains (<b>LDKQSDTQYV</b> and <b>EVQYAGTDGPCK</b>). For T cell epitopes, <b>MMLELDPPF</b> epitope was highly recommended as therapeutic peptide vaccine to interact with MHC class I along with three other epitopes (<b>MAVLGDTAW</b>, <b>KEWFHDIPL</b> and <b>DTAWDFGSV</b>) which showed very good population coverage against the whole world population. Three epitopes showed high affinity to interact with MHC class II alleles (<b>FKSLFGGMS</b>, <b>LITANPVIT</b> and <b>VHTALAGAL</b>) with excellent population coverage throughout the world and South America region. Herd immunity protocols can be achieved in countries with low population coverage percentage to minimize the active transmission of the virus, especially among pregnant women and other groups at risk.We recommend <i>in vitro</i> and <i>in vivo</i> proving the effectiveness of these proposed epitopes as a vaccine, as well as to be used as a diagnostic screening test.},
doi={10.12691/ajbr-4-3-1}
publisher={Science and Education Publishing}
}