@article{ajbr2016411,
author={{Zhang, Gang and Zhang, Yi},
title={On the "All or Half" Law of Recombinant DNA},
journal={American Journal of Biomedical Research},
volume={4},
number={1},
pages={1--4},
year={2016},
url={http://pubs.sciepub.com/ajbr/4/1/1},
issn={2328-3955},
abstract={Plasmid vectors are one of the most important tools for the investigation of the functions of genes of interest. Efficient cloning of various vectors, according to different purposes, is critical for biomedical research. Previously, we reported a new method, designated as ˇ°Combinatorial Strategyˇ±, for cloning different vectors with various clone sites. We demonstrated that it is a quantitative law for recombinant DNA with our method, which is when two different clone sites are used, almost 100% transformants are positive clones, on the other hand, when one over-hang clone site, or different blunt clone sites are used, about 50% of the transformants are positive clones. We named this quantitative law as the "All or Half" law of recombinant DNA. Here, we summarized the mechanisms of recombinant DNA, provided a general protocol and suggested the predicted results for plasmid vector cloning. This is a revolutionary breakthrough of recombinant DNA technology.},
doi={10.12691/ajbr-4-1-1}
publisher={Science and Education Publishing}
}