@article{ajbr2015313,
author={{Muthiadin, Cut and Natsir, Rosdiana and Agus, Rosana and Nasrum, Muhammad and Dwiyanti, Ressy and Sabir, Muhammad and Yasir, Yadi and Hatta, Mochammad},
title={Identification and Characterization of Antigenic 36 Kda Outer Membrane Protein (OMP) of <i>Salmonella entericaserovar</i><i> </i>Typhi (<i>S.typhi</i>) from Makassar, South Sulawesi, Indonesia},
journal={American Journal of Biomedical Research},
volume={3},
number={1},
pages={9--12},
year={2015},
url={http://pubs.sciepub.com/ajbr/3/1/3},
issn={2328-3955},
abstract={Typhoid fever caused by Salmonella entericaserovar Typhi (S. typhi), still remains a public health concern. A simple, rapid and early diagnostic test has been a long felt need for clinicians. Adh036 protein, discovered from Malang, Indonesia, has a candidate vaccine potential for Typhoid Fever. This study aims towards identification and characterization of 36 kda Outer Membrane Protein (OMP) from Makassar, South Sulawesi, Indonesia as a generalization protein test. Isolated bacteria were cultured in MacConkey medium and Brain Heart Infusion Broth (BHIB), and then harvested by using sarcosyl and sonication methods. Then, crude protein was purified by dialysis method using fractional ammonium sulfate precipitation and the protein contents were analyzed using sodium dodecyl sulfate polyacrylamide gel electrophores (SDS-PAGE) for identification it¡¯s molecular weight. The results, from five purified proteins, only three fractions show band at 36 kda, were 0-20%, 20-40%, and 40-60%.},
doi={10.12691/ajbr-3-1-3}
publisher={Science and Education Publishing}
}