eng Science and Education Publishing American Journal of Biomedical Research 2328-3955 2013-11-15 1 4 120 131 10.12691/ajbr-1-4-8 AJBR2013148 article Jiao Chen 1 Yifang Liu 1 Mi Fang 1 Hui Chen 1 Xingzhen Lao 1 Xiangdong Gao 1 Heng Zheng zhengh18@hotmail.com, (W. Yao) 1 Wenbing Yao zhengh18@hotmail.com, (W. Yao) 1 School of Life Science and Technology, China Pharmaceutical University, Nanjing, P.R. China Metallo-¦-lactamases can hydrolyze a broad range of ¦-lactam antibiotics and no effective inhibitors could be used in the clinic. Therefore, the discovery of metallo-¦-lactamase inhibitors has attracted much attention in recent years. In this study, a support vector machine (SVM) that separates compounds into positives and negatives, combined with docking method was employed for virtual screening of IMP-1 metallo-¦-lactamase inhibitors. Eight of the twenty five selected compounds were purchased for in vitro assays. Among them, four compounds show inhibitory potency against IMP-1. Two of them are found to have novel scaffolds, implying a good potential for further optimization. http://pubs.sciepub.com/ajbr/1/4/8/ajbr-1-4-8.pdf IMP-1 metallo-¦-lactamase inhibitorssupport vector machinedockingvirtual screeningin vitro assays