eng Science and Education Publishing Applied Ecology and Environmental Sciences 2328-3920 2021-06-18 9 6 599 606 10.12691/aees-9-6-4 AEES2021964 article Blandine Pulch¨¦rie Tamatcho Kweyang tamatcho@yahoo.com 1 2 Antoine Tamsa Arfao 2 Pierre Ren¨¦ Fotsing Kwetche 3 Luciane Marlyse Moungang 3 Mamert Fils Onana 4 Sim¨¦on Tchakont¨¦ 4 Mireille Ebiane Nougang 5 Olive Vivien Noah Ewoti 5 Ajeagah Gideon Aghaindum 5 Department of Microbiology, University of Yaounde 1, Faculty of Sciences, P.O. Box 812 Yaounde, Cameroon Higher Institute of Health Science, Universit¨¦ des Montagnes; Bangangt¨¦, Cameroon Department of Aquatic Ecosystems Management, Institute of Fisheries and Aquatic Sciences, University of Douala, Douala, Cameroon Department of Biological Sciences, Faculty of Sciences, University of Maroua, P.O Box. 814 Maroua, Cameroon The isolation and characterization of Vibrio strains were carried out in Yaounde (equatorial area). Well water characteristic focused on water temperature, pH, dissolved oxygen, dissolved CO2, electrical conductivity, suspended solids. Vibrio cholerae, Vibrio fluvialis, Vibrio mimicus, Vibrio parahaemolyticus and Vibrio vulnificus were identified in all four wells investigated. On 50 strains of Vibrio cholerae tested, 8 agglutinated anti O1 serum; 16% therefore belong to serogroup O1. Antimicrobial susceptibility tests showed that most of the environmental strains of Vibrio cholerae studied are sensitive to the majority of the antibiotics tested. The percentage of sensitivity for most of the antibiotics tested is above 50%. CPR (98%); CTX (94%); GM (84%); AMC (82%); DOX (72%); AMX (70%); NAL (52%). There were 34% cases of resistant to nalidixic acid, 26% to amoxicillin, 24% to chloramphenicol, and 18% to doxycycline. The percentage of sensitivity of Vibrio cholerae O1 is always greater than 50% with GM (100%); AMX and DOX (87.5%); CTX and CPR (75%); AMC and NAL (62.5%); CHL (50%). These results contribute to prevention and control of cholera in equatorial region. http://pubs.sciepub.com/aees/9/6/4/aees-9-6-4.pdf isolation characterization Vibrio strains antimicrobial susceptibility physico-chemical parameters well