eng Science and Education Publishing Applied Ecology and Environmental Sciences 2328-3920 2021-05-20 9 5 550 556 10.12691/aees-9-5-6 AEES2021956 article A. V. Rama Subbaiah ramscvgl@gmail.com 1 Atmakuru Ramesh 1 Department of Analytical Chemistry, International Institute of Bio-technology and Toxicology (IIBAT), Padappai-601 301, University of Madras, Chennai, Kancheepuram District, Tamil Nadu, India Oxyfluorfen widely using in paddy field and unknowingly polluting the soil. The present study soil microorganism¡¯s carbon and nitrogen transformation test was conducted in loamy sand soil, the experiment conducted as per Organization for Economic Co-operation and Development (OECD) Guideline 216 and 217. The application of oxyfluorfen 23.5% EC applied dose rates T0-Control, T1-100 g a.i/ha (active ingredient/hectare) and T2-1000g a.i/ha soil dry weight. The test concentrations were one and ten times the recommended field application rate. After application of the soil boxes were incubated at 20°C in dark conditions. The soil samples were collected at 0 (within the 6 hours), 7, 14, and 28 days after imposing the treatment. Carbon transformation (short-term respiration) was determined by amended the glucose to soil samples. Short-term respiration was calculated based on oxygen consumption (BOD) during the experiment. Soil nitrification was determined by measuring the NO3- contents of aqueous soil extracts using calibrated ion-sensitive electrodes and the Orion expandable ion analyzer. The deviation in respiration rates and nitrification compared to controls after applying the test item after 28 days 2.8 % and 7.0 % for the; -3.12% and -7.18% for the respiration rates and nitrification respectively T1 and T2 test concentrations. There was no significant variation between the treatment and control samples. http://pubs.sciepub.com/aees/9/5/6/aees-9-5-6.pdf carbon nitrogen HPLC and Loamy sand