eng Science and Education Publishing Applied Ecology and Environmental Sciences 2328-3920 2021-10-14 9 10 895 903 10.12691/aees-9-10-7 AEES20219107 article Vinay V. Chaugule vinaychougule033@gmail.com 1 UG and PG Department of Microbiology, Miraj Mahavidyalaya Miraj, Sangli 416 410, [MS] India Biological Copper oxide nanoparticles (CuO-NPs) were produced utilizing biomass extract of two endophytic actinomycetes isolates, Streptomyces noursei (A-1) and Streptomyces fradiae (A-2). The molecular identification of these two actinomycetes species was accomplished using 16S RNA sequence analysis. UV-Visible spectroscopy, X-ray diffraction (XRD), Fourier transform infrared (FTIR) spectroscopy, and transmission electron microscopy (TEM) were utilized to characterize these biological CuO-NPs. The surface Plasmon resonance (SPR) absorption band of biologically synthesized CuO-NPs was in the spectrum of 410 to 450 nm. These NPs were spherical and crystalline in morphology, with a mean range of 50 to 100 nm. XRD analysis was employed to investigate the crystallinity of these NPs. Different bonds, such as C-O and N-H bonds, were identified in these NPs, as revealed by FTIR spectra. When it came to medicinal applications, the biosynthesized CuO-NPs were remarkably efficacious. The biologically synthesized CuO Nanoparticles (A-1 and A-2) shows the good Scavenging activity which indicated that biologically synthesized CuO Nanoparticles (A-1 and A-2) has an antioxidant property. Prepared biosynthesized Cu oxide nanoparticles are biologically active and will becomes a foundation in the different field of biotechnological applications including environmental, industrial, medical etc. http://pubs.sciepub.com/aees/9/10/7/aees-9-10-7.pdf Triticum vulgare Actinomycetes Cu SO₄ 5H₂O Scavenging