eng Science and Education Publishing Applied Ecology and Environmental Sciences 2328-3920 2020-07-10 8 5 269 272 10.12691/aees-8-5-12 AEES20208512 article Murugan Perachiselvi 1 J. Jenson Samraj 1 Muthiah Sakthi Bagavathy 1 E. Pushpalaksmi 1 G. Annadurai gannadurai@msuniv.ac.in 1 Division of Nanoscience, Sri Paramakalyani Centre for Excellence in Environmental Sciences, Manonmaniam Sundaranar University, Alwarkurichi 627 412, India Currently Nanoparticles are utilized in various fields and have a different clinical application. In our research, we have developed the Cobalt oxide (Co3O4) NPs using the Sol-gel technique from the Cobalt chloride precursor. The synthesized nanoparticle was characterized by using X-Ray Diffraction (XRD), Scanning Electron Microscope (SEM), Dynamic Light Scattering (DLS), and Fourier Transform Infra-Red Spectroscopy (FTIR). The cytotoxicity studies were evaluated on the Vero cell line (African green monkey kidney cell line) by using MTT assay at 72 hrs. The Cell viability which was observed during the process depends upon the concentration and time exposure. Moreover, the In-vitro cytotoxic effects of Co3O4 NPs showed a better result at the concentration of 50 µg/100µl in Vero cell. http://pubs.sciepub.com/aees/8/5/12/aees-8-5-12.pdf cobalt oxide NPs sol-gel technique MTT assay cytotoxicity vero cell line