@article{aees20221074,
author={{Grace, S. Priya and Kumar, A. Ganesh and Srinivasan, T.},
title={<i>In</i><i>-</i><i>vitro</i> Antitoxin Activity of <i>Pittosporum n</i><i>eelgherrense </i>Aqueous Bark Extract against <i>Daboia r</i><i>usselii </i>Venom},
journal={Applied Ecology and Environmental Sciences},
volume={10},
number={7},
pages={453--460},
year={2022},
url={http://pubs.sciepub.com/aees/10/7/4},
issn={2328-3920},
abstract={Snake envenomation is a severe and significant global health problem. Until now polyvalent horse antivenom is the only treatment available for snake bite. Many side effects like anaphylaxis, pyrogen reactions and serum sickness is seen on administering horse antivenom. The ancient ayurvedic system of India refers to the usage of plants in management of snake envenomation. In the present investigation, antitoxin activity of <i>Pittosporum neelgherrense </i>aqueous bark extract was tested by in-vitro approaches on <i>Daboia russelii </i>venom.<i> </i>The bark extracts from<i> P. neelgherrense</i> were used to evaluate the in-vitro<i> </i>neutralization tests such as protease, direct haemolysis assay, phospholipase activity, procoagulant, phosphomonoesterase and phosphodiesterase activity. Gas chromatography-mass spectrometry (GC-MS) analysis from bark extracts was also performed to investigate the bioactive compounds. The <i>in vitro</i> neutralization assays showed the aqueous bark extract contains some bioactive compounds which effectively neutralises the venom induced activities. GC-MS analysis of the plant extract revealed the presence of antivenom compounds which have neutralizing properties on <i>D. russelii</i> venom.},
doi={10.12691/aees-10-7-4}
publisher={Science and Education Publishing}
}