eng Science and Education Publishing Applied Ecology and Environmental Sciences 2328-3920 2022-12-30 10 12 784 789 10.12691/aees-10-12-12 AEES2022101212 article Sonali S. Patil sonaligayu285@gmail.com 1 Department of Chemistry, J.S.M. College, Alibag, Raigad, Maharashtra, India In past few years world is facing serious issue of increased pollution due to heavy toxic metals. In order to minimize such issues an appropriate novel compound was developed for determination of zinc from the various sources of samples. The present work demonstrates the development of new ligand 2,-4-dimethyl-3H-1, 5 benzodiazepine (DBA) and its application in the zinc estimation using spectrophotometric extraction technique. In the Laboratory, synthesis of novel reagent and its characterization has been also performed using mass spectrophotometer and IR, NMR. The reaction between Zinc metal and novel reagent (DBA) produces colored compound, this red colored compound then subjected to extraction process by monitoring constant pH 8.5 with n-butanol as a best solvent. The optimum values of several experimental parameters like highest absorption, extinction coefficient and sandell¡¯s sensitivity of the red colored compound reported to be 540 nm, 5063 L mol-1cm-2 and 0.01304 ¦̀g cm-2, respectively. In a limit of selective concentration range the Beers law is also validated. The developed analytical reagent is found to be best, simple and efficient over the earlier reported reagents. Application of the novel analytical reagent for removal of zinc ions, operating at optimal conditions revealed best results in the real use. http://pubs.sciepub.com/aees/10/12/12/aees-10-12-12.pdf Zn ions spectrophotometric extraction novel analytical reagent beers law