eng Science and Education Publishing Applied Ecology and Environmental Sciences 2328-3920 2022-11-22 10 11 685 690 10.12691/aees-10-11-4 AEES202210114 article Sonali S. Patil sonaligayu285@gmail.com 1 Department of Chemistry, J.S.M. College, Alibag, Raigad, Maharashtra, India The present work addresses, a development of novel analytical reagent 2, 4-dimethyl -3H-1, 5 benzodiazepine (DBA) for determination of rhodium (III) from a mixture of alloy, using extractive spectrophotometric technique. In this research, a novel analytical reagent has been explored and with NMR, IR and mass spectrometer techniques, its characterization was also reported. A reaction between Rhodium (III) and analytical reagent (DBA), resulted in to red-coloured compound, this red colored compound offers outstanding results at constant pH 8.9, when subjected to an extraction using n-butanol as selective solvent. The Beers law is fully satisfied in concentration range of 1 mg L-1 to 10 mg L-1 of rhodium (III) ions. The values of maximum absorption, average molar absorption co-efficient to the coloured compound were observed to be 510 nm, 4863L mol-1cm-2, however sandell¡¯s sensitivity was recorded as 0.01205 ¦̀g cm-2. The determination of rhodium (III) metal traces from alloy mixture using DBA analytical reagent is revealed to be cheaper, effective and best than that of earlier spectrophotometric extraction methods. http://pubs.sciepub.com/aees/10/11/4/aees-10-11-4.pdf rhodium (III) Spectrophotometric estimation DBA reagent molar extinction coefficient