Science and Education Publishing Applied Ecology and Environmental Sciences 1 1 2013 05 30 Changes in Enzymes Activities of Clarias Gariepinus Brood Fish Exposed to Anaesthetics Metomidate 37 40 EN O.A. Akinrotimi African Regional Aquaculture Centres/Nigerian Institute for Oceanography and Marine Research. Port Harcourt, Rivers State, Nigeria U.U Gabriel O.O. Orokotan AEES2013133 10.12691/aees-1-3-3 2013 01 01 2013 05 29 2013 05 30 The effect of anaesthetic metomidate on the enzymes activities (Alanine transminase ¨C ALT; Aspartate transaminase ¨C AST; Alkaline phosphatase-ALP and Lactate dehydrogenase ¨C LDH) in female Clarias gariepinus was investigated using different concentrations (0.00mlL^-1 ¨C control; 0.25; 0.75; 1.00; 2.00; 6.00; 8.00; 10.00 and 12.00mlL^-1) in triplicates. The results from the study indicated that the anaesthetic caused a concentration dependent significant increase (p < 0.05) in the four enzymes under consideration. The highest activities in all the enzymes were observed in 12.00mlL^-1 and the lowest in the control. The results from this work therefore suggests that the anaesthetics impair enzymes activities in the fish which was more noticeable in the fish exposed to higher concentration of 8.00-12.00m/L^-1. Hence caution should be exercised in the application of this anaesthetics in sedation of C. gariepinus.