Nanoscience and Nanotechnology Research

Current Issue» Volume 2, Number 1 (2014)

Article

Effect of the Electric Field on the Antibacterial Activity of Au Nanoparticles on Some Gram-positive and Gram-negative Bacteria

1Department of Physics, College of Sciences, AL-Nahrain University, Baghdad, Iraq

2Department of Biotechnology, College of Sciences, AL-Nahrain University, Baghdad, Iraq

3Department of Physics, College of Sciences, Baghdad University, Baghdad, Iraq


Nanoscience and Nanotechnology Research. 2014, 2(1), 1-7
DOI: 10.12691/nnr-2-1-1
Copyright © 2104 Science and Education Publishing

Cite this paper:
Thamir Jumaa, Maysaa Chasib, Mazin K. Hamid, Raad Al-Haddad. Effect of the Electric Field on the Antibacterial Activity of Au Nanoparticles on Some Gram-positive and Gram-negative Bacteria. Nanoscience and Nanotechnology Research. 2014; 2(1):1-7. doi: 10.12691/nnr-2-1-1.

Correspondence to: Maysaa  Chasib, Department of Biotechnology, College of Sciences, AL-Nahrain University, Baghdad, Iraq. Email: dr.maysaa78@yahoo.com

Abstract

Metal nanoparticles are being extensively used in various biomedical applications due to their small size to volume ratio and extensive thermal stability. Gold nanoparticles (AuNPs) are an obvious choice due to their amenability of synthesis and functionalization, less toxicity and ease of detection. The synthesis and bioactivity of gold nanoparticles has been extensively studied. The present study was focused on method to increase the activity and the efficacy of the antibacterial activity of gold nanoparticles which produced by laser ablation of 1064 nm wavelength and three energy powers (400,500,600) mJ were applied to produced gold nanoparticles with different sizes on Gram-positive isolate (Staphylococcus aureus) and the Gram-negative isolate(Pseudomonas aeroginosa). It was found that using the agar well diffusion assay method which showed that the individually of AuNPs of 0.2 mg/ml concentration have no synergistic effect on the studied Staphylococcus aureus and Pseudomonas. So, a new modified technique was made on these AuNPs with the same concentration to increase their antibacterial activity, is exposure the gold nanoparticles colloidal to 1500 v/m applied electric field which resulting to be an effective AuNPs with inhibition properties against Gram-positive isolates (Staphylococcus aureus) contrary to nanoparticles that was not exposed to electric field with the same concentration.

Keywords

References

[[[[[[[[[
[1]  KOWSHIK, M.; ASHTAPUTRE, S.; KHARRAZI, S. Extracellular synthesis of silver nanoparticles by a silver-tolerant yeast strain MKY3. Nanotec, v. 14, n. 1, p. 95-100, 2003.
 
[2]  CHITRANI, B.D.; GHAZANI, A.A.; CHAN, W.C.W. Determining the size and shape dependence of gold nanoparticle uptake into mammalian cells. Nano Lett, v. 6, n.4, p. 662-668, 2006.
 
[3]  PISSUWAN, D.et al. Functionalised gold nanoparticles for controlling pathogenic bacteria. Trends in Biotechnology, v. 28, n.4, p. 207-13, 2010.
 
[4]  Strasak L, Vetterl V, Smarda J. Effects of low-frequency mAunetic fields on bacteria Escherichia coli. Bioelectrochemistry 2002; 55: 161-4.
 
[5]  C. F. Bohren and D. R. Huffman, Absorption and Scattering of Light by Small Particles~Wiley Interscience, New York, 1983.
 
Show More References
[6]  U. Kreibig and M. Vollmer, Optical Properties of Metal Clusters; Springer Series in Materials Science 25~Springer, Berlin, 1995.
 
[7]  Henglein, A. and Giersig, M., J. Phys. Chem. B., 104: 6767(2000).
 
[8]  Mclntosh, R.M. Laboratory Manual Experimental Microbiology.1st edition, Mosby-Year bok, Inc. (1996).
 
[9]  Mahmoud, M.J.; Jawad, A.J.; Hussain, A.M.; Al-Omeri, M.; and Al- Naib, A.; Invitro Antimicrobial activity of Sasolia rosmarinus and Adiantum capillusveneris. Int., J. Crude. Drug. Res. 27:14-16(1989).
 
[10]  Fricker S.P.; Medical Uses of Gold Compounds: Past, Present, Future, Gold Bulletin, 1996, 29(2) 53-64.
 
[11]  Grace NA, Pandian K: Antibacterial efficacy of aminoglycosidic antibiotics protected gold nanoparticles – A brief study. Colloids and surface A: Physico chem. Eng Aspects 2007; 297: 63-70.
 
[12]  Sobczak-Kupiec A, Malina D, Zimowskaa M, Wzorek Z: Characterization of gold nanoparticles for various medical applications. Dig J Nanomater Bios 2011; 6(2): 803-808.
 
[13]  O.R. Musaev, A.E. Midgley, J.M. Wrobel, M.B. Kruger (Laser ablation of alumina in water) Chemical Physics Letters 487 (2010) 81-83.
 
[14]  Rai A, Prabhune A, Perry CC. Antibiotic mediated synthesis of gold nanoparticles with potent antimicrobial activity and their application in antimicrobial coatings. J. Mater. Chem. 2010; 20: 6789-6798.
 
Show Less References