ISSN (Print): 2374-1996

ISSN (Online): 2374-2003


Editor-in-chief: Jean Rommelaere

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Currrent Issue: Volume 4, Number 2, 2016


Ameliorative Potential of Different Doses of Indol-3-carbinol on Doxorubicin-induced Cardiotoxicity in Mice

1Pharmacology department, Faculty of Medicine, Zawia University, Lybia

2Pharmacology and toxicology department, Faculty of Pharmacy, Helwan University, Egypt

3Pharmacology department, Faculty of Medicine, Tanta University, Egypt

Journal of Cancer Research and Treatment. 2016, 4(2), 26-31
doi: 10.12691/jcrt-4-2-2
Copyright © 2016 Science and Education Publishing

Cite this paper:
Almokhtar A. Adwas, Abeer A. Elkhoely, Ahmed M. Kabel, Mohamed Nabih Abdel-Rahman, Amany A. Eissa. Ameliorative Potential of Different Doses of Indol-3-carbinol on Doxorubicin-induced Cardiotoxicity in Mice. Journal of Cancer Research and Treatment. 2016; 4(2):26-31. doi: 10.12691/jcrt-4-2-2.

Correspondence to: Ahmed  M. Kabel, Pharmacology department, Faculty of Medicine, Tanta University, Egypt. Email:


Background: Doxorubicin (DOX) is a commonly used chemotherapeutic agent that is associated with serious dose-limiting cardiotoxicity. This cardiotoxicity was attributed to various mechanisms including induction of oxidative stress and inflammation together with inhibition of apoptosis. Indole-3-carbinol (I3C) is a phytochemical that was suggested to have potent anti-oxidant and anti-inflammatory properties. Aim: It was to detect the possible ameliorative effects of different doses of I3C on doxorubicin-induced cardiotoxicity in mice. Methods: Eighty mice were divided into four equal groups: control untreated group; DOX group; DOX + I3C 1000 ppm group and DOX + I3C 2000 ppm group. Survival rate, serum creatine kinase (CK-MB), lactate dehydrogenase (LDH) and troponin I were measured. Also, tissue malondialdehyde (MDA), tissue catalase (CAT), tissue glutathione peroxidase (GPx), and tissue tumor necrosis factor alpha (TNF-α) were determined. Parts of the heart were subjected to histopathological examination. Results: I3C produced dose-dependent significant increase in the survival rate, tissue GPx and CAT with significant decrease in serum CK-MB, LDH, troponin I, tissue MDA and TNF-α and improved the histopathological and immunohistochemical changes compared to DOX-treated group. Conclusion: I3C- in a dose dependent manner- had a protective effect against doxorubicin-induced cardiotoxicity in mice.



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Histone H1.5 Expression in Prostatic Carcinoma: An Immunohistochemical Study

1Pathology Department, Faculty of Medicine, Tanta University, Tanta, Egypt

2Pharmacology department, Faculty of Medicine, Tanta University, Tanta, Egypt

3Department of clinical pharmacy, College of Pharmacy, Taif University, Taif, Saudi Arabia

Journal of Cancer Research and Treatment. 2016, 4(2), 21-25
doi: 10.12691/jcrt-4-2-1
Copyright © 2016 Science and Education Publishing

Cite this paper:
Mohamed A. El-Rashidy, Asmaa E. Bedeer, Ahmed M. Kabel. Histone H1.5 Expression in Prostatic Carcinoma: An Immunohistochemical Study. Journal of Cancer Research and Treatment. 2016; 4(2):21-25. doi: 10.12691/jcrt-4-2-1.

Correspondence to: Ahmed  M. Kabel, Pharmacology department, Faculty of Medicine, Tanta University, Tanta, Egypt. Email:


Background and Aim: Histone H1.5 (HH1.5) is a subtype of histone H1, a family of linker proteins that is known to determine chromatin structure, alter gene expression and DNA repair. It also contributes to regulation of cell proliferation in breast cancer. In this study, we aimed to investigate the immunohistochemical expression of HH1.5 in various prostatic lesions. Methods: A total 50 cases of various prostatic biopsies were studied. Histone H1.5 expression was evaluated in all cases. HH1.5 expression was scored as negative (<11%), 1+ (11-50%), or 2+ (>50%). Correlations between the intensity and differential localization of these markers and Gleason patterns were evaluated. Results: HH1.5 immunohistochemistry revealed positive nuclear reactivity in all cases (100%) of prostate adenocarcinomas, compared to only 2 (11%) of 18 cases of benign prostatic glands (P ≤ 0.001). In all positive benign prostate epithelium, HH1.5 was limited to focal and weak reactivity. Similarly, both the two cases of high-grade prostatic intraepithelial neoplasia exhibited focal weak nuclear reactivity. Increased HH1.5 reactivity was observed in Gleason patterns 5 and 4 as compared to Gleason pattern 3, 100%, 64.7% and 50%, respectively (P ≤ 0.002). Conclusion: HH1.5 may be a useful diagnostic tool in evaluating prostatic biopsies, particularly with small foci of cancer. Further studies are needed to support these findings and investigate the possible prognostic significance of HH1.5 in prostatic adenocarcinomas.



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