ISSN (Print): 2374-1996

ISSN (Online): 2374-2003

Website: http://www.sciepub.com/journal/jcrt

Editor-in-chief: Jean Rommelaere

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Currrent Issue: Volume 4, Number 4, 2016

Article

Oleuropein is a Powerful Sensitizer of Doxorubicin-mediated Killing of Prostate Cancer Cells and Exerts Its Action via Induction of Autophagy

1Department of Nutrition Science and Dietetics, Harokopio University, Athens, Greece

2Division of Pharmaceutical Chemistry, Faculty of Pharmacy, National and Kapodistrian University of Athens, Greece

3Division of Pharmacognosy and Chemistry of Natural Products, Faculty of Pharmacy, National and Kapodistrian University of Athens, Greece

4Laboratory of Cell Proliferation and Ageing, Institute of Biosciences & Applications, National Centre for Scientific Research "Demokritos", Athens, Greece


Journal of Cancer Research and Treatment. 2016, 4(4), 61-68
doi: 10.12691/jcrt-4-4-2
Copyright © 2016 Science and Education Publishing

Cite this paper:
Anastasia Papachristodoulou, Magafoula Tsoukala, Dimitra Benaki, Sarantos Kostidis, Katerina Gioti, Nektarios Aligiannis, Harris Pratsinis, Dimitris Kletsas, Alexios-Leandros Skaltsounis, Emmanuel Mikros, Roxane Tenta. Oleuropein is a Powerful Sensitizer of Doxorubicin-mediated Killing of Prostate Cancer Cells and Exerts Its Action via Induction of Autophagy. Journal of Cancer Research and Treatment. 2016; 4(4):61-68. doi: 10.12691/jcrt-4-4-2.

Correspondence to: Roxane  Tenta, Department of Nutrition Science and Dietetics, Harokopio University, Athens, Greece. Email: rtenta@hua.gr, mikros@pharm.uoa.gr

Abstract

The phenolic component Oleuropein (OLEU), a bioactive natural product, has recently shown antiproliferative properties. Doxorubicin (DXR) is an anthracycline present in many chemotherapeutic schemes, although limited due to its cardio-toxic effects. Important research effort has been devoted therefore, to reducing DXR toxicity without compromising its antitumor efficacy. The anticancer actions of DXR and OLEU were assessed, on PC-3 prostate cancer cells, while cell cycle distribution and rate of apoptosis were assessed by flow cytometry. The autophagic process was determined via immunoblotting and immunofluorescent staining. Finally, cell extracts were analyzed by NMR spectroscopy. The present study showed that both DXR and OLEU inhibited PC-3 cells proliferation, while the co-treatment with DXR and OLEU resulted in an additive inhibition. Although the addition of OLEU to DXR did not alter significantly the cell cycle distribution, exhibited by each treatment alone, and produced a marginal increase on the rate of apoptosis, both compounds produced a remarkable induction of autophagy. In addition, treated cells exhibited significant metabolite alterations. This study demonstrates that OLEU, a basic component of the everyday diet, is capable of lowering significantly the cytotoxic dose of DXR, while obtaining an important anti-proliferative effect in prostate cancer cells.

Keywords

References

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Article

Hepatocellular Carcinoma: A Focus on the New Lines of Management

1Department of Clinical Pharmacy, College of Pharmacy, Taif University, Taif, KSA

2Department of Pharmacology, Faculty of Medicine, Tanta University, Tanta, Egypt

3Final year student, College of Pharmacy, Taif University, Taif, KSA


Journal of Cancer Research and Treatment. 2016, 4(4), 55-60
doi: 10.12691/jcrt-4-4-1
Copyright © 2016 Science and Education Publishing

Cite this paper:
Ahmed M. Kabel, Abrar A. Al-joaid, Afnan A. Al-ghamdi, Aseel A. Al-joaid, Wejdan S. Al-zaidi. Hepatocellular Carcinoma: A Focus on the New Lines of Management. Journal of Cancer Research and Treatment. 2016; 4(4):55-60. doi: 10.12691/jcrt-4-4-1.

Correspondence to: Ahmed  M. Kabel, Department of Clinical Pharmacy, College of Pharmacy, Taif University, Taif, KSA. Email: drakabel@gmail.com

Abstract

Hepatocellular carcinomas (HCCs) are aggressive tumors with a high dissemination rate. Diagnosis of HCC depends on physical examination, computed tomography, magnetic resonance imaging and biopsy results. An early diagnosis of these tumors is of great importance in order to offer the possibility of curative treatment. HCC is relatively insensitive to systemic therapy, radiotherapy or surgery and this usually depends on the stage of the disease. Diagnosis and treatment of HCC has witnessed many major changes and challenges in the past years. New alternative lines of treatment of HCC were developed to attack the tumor at the molecular level to improve the prognosis and achieve better results. These include check-point inhibitor therapy, molecular targeted therapy and angiogenesis inhibitors.

Keywords

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