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Time-dependent Toxic Effect and Distribution of Silver Nanoparticles Compared to Silver Nitrate after Intratracheal Instillation in Rats

1Cellular Functional Nanobiomaterials Group, Research Center for Functional Materials, National Institute for Materials Science (NIMS), Tsukuba, Ibaraki, Japan

2Department of Forensic Medicine and Toxicology, Faculty of Veterinary Medicine, Mansoura University, Mansoura, Egypt

3Department of Pathology, Faculty of Veterinary Medicine, Mansoura University, Mansoura, Egypt

4Graduate School of Advanced Science and Engineering, Waseda University, Tokyo, Japan

American Journal of Nanomaterials. 2016, 4(1), 12-19
doi: 10.12691/ajn-4-1-3
Copyright © 2016 Science and Education Publishing

Cite this paper:
Alaa Fehaid, Mohamed F. Hamed, Mamdouh M. Abouelmagd, Akiyoshi Taniguchi. Time-dependent Toxic Effect and Distribution of Silver Nanoparticles Compared to Silver Nitrate after Intratracheal Instillation in Rats. American Journal of Nanomaterials. 2016; 4(1):12-19. doi: 10.12691/ajn-4-1-3.

Correspondence to: Akiyoshi  Taniguchi, Cellular Functional Nanobiomaterials Group, Research Center for Functional Materials, National Institute for Materials Science (NIMS), Tsukuba, Ibaraki, Japan. Email:


Silver nanoparticles (AgNPs) are widely used because of their anti-bacterial and anti-inflammatory properties; however, the adverse health effects of these nanoparticles, especially to the lungs, have been less studied. We thus investigated the inflammatory response of polyvinylpyrrolidone (PVP)-coated AgNPs and silver nitrate (AgNO3) after 24 h, 14 days and 28 days of single intratracheal instillation in rats. The bronchoalveolar lavage fluid (BALF) samples were collected and analyzed; a significant influx of neutrophils into the lung was found in both treated groups after 24 h with a presence of AgNPs in the alveolar macrophages after 24 h, 14 days and 28 days of instillation. Pro-inflammatory cytokines and enzymatic activities showed a significant increase after 24 h in both treated groups with a higher significance in the AgNO3-treated group than the AgNPs-treated group. After 28 days, these increases were completely recovered in the AgNO3-treated group but were still present in the AgNPs-treated group. The gross examination of lung tissues revealed a clear focal inflammation in the AgNPs-treated group after 28 days. More than 29% and 9% of the initial dose of AgNPs were recovered in lung tissues after 1 day and 28 days, respectively. Comparatively, the AgNO3-treated group recovered only 16.5% and 1%, suggesting that the silver ions are easily absorbed into the circulation and distributed to different tissues more than the nanoparticles. Our results indicated that the PVP-AgNPs caused a subchronic pulmonary inflammation compared to the acute one induced by the ionic form, which can be recovered easily.



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Morphological, Structural and Optical Properties of W Doped-ZnO Films Grown by Pulsed Laser Deposition onto Different Glass Substrates

1Laboratoire de Photonique et de Nano-Fabrication, Groupe de physique du Solide et Sciences des Matériaux, Faculté des sciences et Techniques Université Cheikh Anta Diop de Dakar (UCAD), B.P. 25114 Dakar-Fann Dakar (Senegal)

American Journal of Nanomaterials. 2016, 4(1), 20-26
doi: 10.12691/ajn-4-1-4
Copyright © 2016 Science and Education Publishing

Cite this paper:
A.O. Kane, C. B. Ndao, E. H. O. Gueye, M. B. Gaye, N. M. Ndiaye, I. Ngom, B. D. Ngom, P. D. Tall, A.C. Beye. Morphological, Structural and Optical Properties of W Doped-ZnO Films Grown by Pulsed Laser Deposition onto Different Glass Substrates. American Journal of Nanomaterials. 2016; 4(1):20-26. doi: 10.12691/ajn-4-1-4.

Correspondence to: B.  D. Ngom, Laboratoire de Photonique et de Nano-Fabrication, Groupe de physique du Solide et Sciences des Matériaux, Faculté des sciences et Techniques Université Cheikh Anta Diop de Dakar (UCAD), B.P. 25114 Dakar-Fann Dakar (Senegal). Email:


Tungsten-doped zinc oxide thin films were prepared by ablating a target containing 1 wt% WO3 with XeCl excimer laser (λ=3O8 nm). The films were grown onto different glass substrate at a repetition rate of 10Hz, pulse energy of 100 mJ and irradiation time of 20 min. The structural and optical properties of the films are found to be strongly dependent on the nature of the substrate. The X-ray Diffraction (XRD) results show that all the films are preferentially C-axis oriented. The room temperature photoluminescence (PL) spectrum shows a dominant near-band-edge emission peak for the film deposited on borosilicate and GGG substrate. The average transmittance was found to be in the range of 84-90%. The absorption coefficient exhibits a direct bandgap feature with some band tailing effects.



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Strategy for Boosting Rock Phosphate Efficiency and Conversion into Nano Zeolite

1Agriculture Research Center, Soil, Water& Environment Institute, Giza, Egypt

2Cairo University, Faculty of Agriculture, Plant Physiology department, Giza, Egypt

American Journal of Nanomaterials. 2016, 4(2), 27-38
doi: 10.12691/ajn-4-2-1
Copyright © 2016 Science and Education Publishing

Cite this paper:
Hassan AZA, Abdel Wahab M Mahmoud. Strategy for Boosting Rock Phosphate Efficiency and Conversion into Nano Zeolite. American Journal of Nanomaterials. 2016; 4(2):27-38. doi: 10.12691/ajn-4-2-1.

Correspondence to: Abdel  Wahab M Mahmoud, Cairo University, Faculty of Agriculture, Plant Physiology department, Giza, Egypt. Email:


Present investigation aimed to convert rock phosphate ore into nano zeolite using calcination (from 200 to 700°C) for 48 hrs. and zeolitization(replacement by a zeolite mineral) processes in order to boost rock phosphate ore efficiency as a source of P2O5 even under high soil pHs. Crystallization, phases, physico-chemical characteristics and surface morphology were studied by visual techniques. Using scanning electron microscopy (SEM) coupled with energy dispersive spectroscopy (EDS) which cleared that, the converted rock phosphate gave different shapes and sizes of crystals and distribution of its components (elements and oxides mass percentage). While TEM appears the crystals size of converted rock phosphate was found in nano size (19.7 -39.1 nm). Moreover Sterio microscopy illustrated that, after calcination and zeolitization processes, the nano zeolite converted from rock phosphate ore took different shapes with three dimension crystals growth as end of crystallization process. Whilst XRD manifested that, the dominant mineral was zeolite associated with different minerals represented by mica muscovite, montmorillonite, calcite, pyrite, alkali feldspar, plagoclase feldspar and quartz. At the same time, XRF was used to verify nano rock phosphate converted in to zeolite (heulandites type). Also the XRF analysis recorded Si/Al ratio (3.42%) of nano zeolite its properties tended to both hydrophilic and organophillic. Furthermore, DTA (differential thermal analysis) and TGA (thermo-gravimetric analysis) were used for measure percentages of water molecules attached with nano rock phosphate and its thermal stability. Therewithal, Surface area (BET) was (14.93m2/g), pore sizes distribution from 3.98 to 56.87 nm, pore volume19.96 nm and pore width 4.846 cm3 / g. It was observed that there is little change in particles density in both real and bulk densities. Finally, the highest CEC (cation exchange capacity) and lowest AEC (anion exchange capacity) values of the conversion rock phosphate were acquired. In a nutshell, our results designated for more liberate of P2O5 in available and safety form to uptake easily by cultivated crops, and construct an appropriate media for loading by beneficial microorganisms.



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