American Journal of Microbiological Research

ISSN (Print): 2328-4129

ISSN (Online): 2328-4137

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Website: http://www.sciepub.com/journal/AJMR

   

Article

Antimicrobial Sensitivity Pattern of Escherichia coli Causing Urinary Tract Infection in Bangladeshi Patients

1Department of Microbiology, Delta Medical College

2Department of Nephrology, Bangladesh Medical College & Hospital


American Journal of Microbiological Research. 2016, 4(4), 122-125
doi: 10.12691/ajmr-4-4-3
Copyright © 2016 Science and Education Publishing

Cite this paper:
Nayareen Akhtar, Rezwanur Rahman, Shahin Sultana. Antimicrobial Sensitivity Pattern of Escherichia coli Causing Urinary Tract Infection in Bangladeshi Patients. American Journal of Microbiological Research. 2016; 4(4):122-125. doi: 10.12691/ajmr-4-4-3.

Correspondence to: Nayareen  Akhtar, Department of Microbiology, Delta Medical College. Email: nayareen07@gmail.com

Abstract

Objectives: Urinary tract infection (UTI) is a common bacterial infection in the Bangladesh community. There has been an increasing resistance by Escherichia coli to the commonly available antibiotics. The aim of the present study was to determine the prevalence of UTI, the common causative bacteria & antimicrobial susceptibility patterns of E. coli responsible for urinary tract infections (UTIs) to currently used antimicrobial agents. Methods and Results: In this study, three hundred urine specimens from clinically suspected UTI patients were collected from both outpatient and inpatient department during the period of February 2015 to January 2016 from a tertiary level hospital in the central part of country. The inclusion criteria included patients presenting with symptoms suggestive of UTI at the study site and who gave informed written consent to participate in the study. The exclusion criteria included patients on antibiotics within the last 2 weeks, and those with recent history of instrumentation. The urine samples received were processed using standard methods. Antimicrobial sensitivity patterns were performed on all E. coli isolates obtained from urine samples by disc diffusion method. Among 300 urine samples, (59%) yielded significant bacteriuria; 123 samples (41%) showed no growth. Out of 177 urine samples which showed significant bacterial growth, 72 (40.7%) samples comprised of males and 105 (59.3%) of females. Females within the age group of 20–29 years(26.67%) and elderly males of ≥60 years(34.7%) showed higher prevalence of UTI. 75.7% of isolates were found to be Escherichia coli, 7.9% Klebsiella pneumoniae, 5.6% Proteus mirabilis, Pseudomonas aeruginosa 5.1%, 1.7% Enterococci faecalis, 2.8% Staphylococci saprophyticus and 1.1% were Staphylococcus aureus. E. coli as the predominant cause of UTI, showed the highest percentage of resistance to co-trimoxazole, nalidixic acid and amoxicillin. The isolates were most sensitive to Imipenam, Meropenam, Nitrofurantoin and Amikacin. Klebsiella pneumoniae was the second most prevalent pathogen. Conclusion: E. coli was the most frequent isolate. Imipenam, Meropenam, Nitrofurantoin and Amikacin were shown to be very effective against E. coli organisms.

Keywords

References

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Article

Antibiotics Susceptibility Pattern and Plasmid Profile of Bacteria Isolated from Public Motorcycle Helmets

1Department of Microbiology, Federal University of Agriculture, PMB 2240 Abeokuta, Nigeria

2Department of Biological Sciences, Yaba College of Technology, PMB 2011, Lagos, Nigeria

3Department of Medical Microbiology and Parasitology, College of Medicine, University of Lagos, PMB 12003, Idiaraba, Lagos, Nigeria

4Molecular Biology and Biotechnology Division, the National Institute of Medical Research, PMB 2013, Yaba, Lagos, Nigeria


American Journal of Microbiological Research. 2016, 4(4), 126-131
doi: 10.12691/ajmr-4-4-4
Copyright © 2016 Science and Education Publishing

Cite this paper:
Obinna Ezeokoli, Leonard Adamu, Faustina Ezeamaramu, Godwin Ovioma, Joy Oladele, Muinah Fowora, Margaret Ugo-Ijeh, Jude Iyile. Antibiotics Susceptibility Pattern and Plasmid Profile of Bacteria Isolated from Public Motorcycle Helmets. American Journal of Microbiological Research. 2016; 4(4):126-131. doi: 10.12691/ajmr-4-4-4.

Correspondence to: Leonard  Adamu, Department of Biological Sciences, Yaba College of Technology, PMB 2011, Lagos, Nigeria. Email: unileonard@yahoo.com

Abstract

The shared use of motorcycle helmets amongst commuters could serve as a source of spread of antibiotic resistant bacteria. In this study, the antibiotic susceptibility pattern and plasmid profile of bacteria isolated from motorcycle helmets in Lagos, Nigeria were investigated. Bacteria were isolated from forty randomly sampled motorcycle helmets and characterized using morphological and biochemical tests. A total of 83 isolates belonging to the phyla Firmicutes (74.7%) and Proteobacteria (25.3%) were obtained, and included species such as Bacillus subtilis, Bacillus anthracis, Escherichia coli, Klebsiella spp., Pseudomonas aeruginosa, Salmonella spp., Shigellla spp., Staphylococcus aureus, and Staphylococcus spp. We identified species with multiple resistance patterns to commonly used antibiotics such as the β-lactams: augumentin, amoxicillin and cloxacilin, as well as the broad spectrum antibiotics gentamicin. The calculated multiple antibiotic resistance index ranged from 0.3 to 1.0. A number of the isolated species had plasmid DNA which on curing, influenced the overall sensitivity of bacteria to antibiotics. These results suggests (without outright proof) presence of antibiotic resistant plasmids in commercial motorcycle helmets and points to the possible role of plasmids in the response of bacteria to antibiotics tested. Findings of this study further highlights the epidemiological significance of motorcycle helmets sharing amongst commuters.

Keywords

References

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Article

Synergistic Effect of Biogenic Silver-nanoparticles with β. lactam Cefotaxime against Resistant Staphylococcus arlettae AUMC b-163 Isolated from T3A Pharmaceutical Cleanroom, Assiut, Egypt

1Department of Botany and Microbiology, Faculty of Science, Assiut University, Egypt


American Journal of Microbiological Research. 2016, 4(5), 132-137
doi: 10.12691/ajmr-4-5-1
Copyright © 2016 Science and Education Publishing

Cite this paper:
M. H. A. Hassan, M.A. Ismail, A.M. Moharram, A. Shoreit. Synergistic Effect of Biogenic Silver-nanoparticles with β. lactam Cefotaxime against Resistant Staphylococcus arlettae AUMC b-163 Isolated from T3A Pharmaceutical Cleanroom, Assiut, Egypt. American Journal of Microbiological Research. 2016; 4(5):132-137. doi: 10.12691/ajmr-4-5-1.

Correspondence to: A.  Shoreit, Department of Botany and Microbiology, Faculty of Science, Assiut University, Egypt. Email: ahmedshoreit@yahoo.com

Abstract

The aim of this study was to biosynthesis silver nanoparticles (AgNPs) from Staphylococcus arlettae AUMC b-163 isolated from T3A pharmaceutical company cleanroom, its antimicrobial activity, and the synergistic effect of AgNPs in combination with commonly used antibiotic Cefotaxime sodium against resistant bacteria. The synthesized AgNPs from bacterial were characterized by using UV-VS spectrophotometer analysis, Fourier Transform Infrared Spectroscopy (FTIR), X-ray diffraction (XRD) and Transmission Electron Microscopy (TEM).UV-VS spectrophotometer analysis showed a peak at 420 nm corresponding to the Plasmon absorbance of silver nanoparticles and FTIR analysis showed the potential biomolecule responsible for the reduction of silver. The structural properties of silver nanoparticles were confirmed using XRD technique, while TEM micrographs revealed that the silver nanoparticles are dispersed and aggregated, and mostly having spherical shape within the size range between 8 and 35 nm. The synthesized silver nanoparticles exhibited a varied growth inhibition activity against the tested pathogenic bacteria. A significant increase in area of growth inhibition was observed when a combination of silver nanoparticles and Cefotaxime antibiotics was applied. The current results revealed that the synthesized silver nanoparticles produced by the bacterial strain Staphylococcus arlettae AUMC b-163 is a promising to be used in medical therapy due to their broad spectrum against some pathogenic bacteria, fungi and resistant tested bacteria.

Keywords

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