American Journal of Microbiological Research

ISSN (Print): 2328-4129

ISSN (Online): 2328-4137

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Website: http://www.sciepub.com/journal/AJMR

   

Article

In Vitro Antibacterial Activity of Some Natural and Trade Iraqi Honey against MRSA Staphylococcus Heamolyticus Isolated from Some Burned Patients in Misan City

1College of Science, Misan University, Iraq

2College of Basic Education, Misan University, Iraq


American Journal of Microbiological Research. 2016, 4(5), 159-163
doi: 10.12691/ajmr-4-5-6
Copyright © 2016 Science and Education Publishing

Cite this paper:
Mohammed A. Abd Ali, Sailh M. Kidem, Ali A. Fadhil, Nusaybah KH.Saddam. In Vitro Antibacterial Activity of Some Natural and Trade Iraqi Honey against MRSA Staphylococcus Heamolyticus Isolated from Some Burned Patients in Misan City. American Journal of Microbiological Research. 2016; 4(5):159-163. doi: 10.12691/ajmr-4-5-6.

Correspondence to: Mohammed  A. Abd Ali, College of Science, Misan University, Iraq. Email: waleed.abas22@yahoo.com

Abstract

The study is aimed to evaluate the antibacterial activity of both natural Iraqi honeys (Sidr, Eucalyptus) and trade honey sample against MRSA Staphylococcus heamolyticus isolated from burned patients in alsader hospital-misan city then were analyzed between (October to December in 2015). The samples were taken in order to determine the bacterial profile and antibiotic susceptibility. The isolates of Staphylococcus heamolyticus were tested against 5 different antibiotics by a disk diffusion method 100% of the Staphylococcus heamolyticus isolates were resistant to the Optochin, Lincomycin, Ampicillin, Amphotercin, while the inhibitory effect of Amikacin antibiotic were (24mm) on MRSA Staphylococcus heamolyticus. Total antibacterial activity was evaluated by measuring the clear zone around the well. Honey samples were tested in different concentration (75%, 50 %, 25%, 10% and 100%). However, the results showed the absences of inhibitory zone to both natural and trade honeys type on MRSA Staphylococcus heamolyticus tested bacteria after dilution of(25% Sidr honey sample, 50% Eucalyptus honey sample, 50% Trade honey sample), while Sidr honey sample have more effective in 75%v/v concentration against MRSA Staphylococcus heamolyticus tested bacteria. The minimum inhibitory concentration (MIC) of the Sidr honey sample were also determined. Finally cytotoxicity evaluating toward human RBC, the results revealed the Iraqi honeys sample have not any cytotoxicity in all concentration. The good antimicrobial potency of sidr and Eucalyptus Iraqi honeys could potentially be used as therapeutic against MRSA Staphylococcus heamolyticus as an alternative to the costly antibiotics.

Keywords

References

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Article

Prevalence of Plasmodium vivax Malaria among Children in Kassala State, Eastern Sudan

1Faculty of Medicine, University of Kassala, Kassala, Sudan

2College of Medicine, University of Bisha, Bisha, Saudi Arabia

3Faculty of Medicine, Al-Neelain University, Khartoum, Sudan


American Journal of Microbiological Research. 2016, 4(6), 164-167
doi: 10.12691/ajmr-4-6-1
Copyright © 2016 Science and Education Publishing

Cite this paper:
Mamoun Magzoub, Mustafa A Alkawad, Mohamed A Mohamed, Hamza M Ali, Abdallah M Osman, Elhady E Elmubarak, Shamselfalah M Osman, Osman E Osman. Prevalence of Plasmodium vivax Malaria among Children in Kassala State, Eastern Sudan. American Journal of Microbiological Research. 2016; 4(6):164-167. doi: 10.12691/ajmr-4-6-1.

Correspondence to: Mamoun  Magzoub, Faculty of Medicine, University of Kassala, Kassala, Sudan. Email: mosmanmm@hotmail.com

Abstract

Background: All over the world 2.6 billion people per year are at risk of Plasmodium vivax malaria, 56% of all malaria cases outside Africa are due to Plasmodium vivax, also about 70-80 million malaria cases per year worldwide are due to Plasmodium vivax. To the best of our knowledge, there is no epidemiological study assessing the prevalence of Plasmodium vivax malaria in Sudan. Methods: This retrospective study was carried out at Kassala state, eastern Sudan. Thick and thin blood films were prepared from capillary blood sample from two thousand, eight hundred and seven febrile patients. The slides were stained with Giemsa and screened for the presence of malaria parasites and parasite species. Stained blood films were examined microscopically with (X100) oil immersion lens. Results: Plasmodium vivax was detected in 41 blood samples (1.5 %) when examined microscopically. Most of them 28 (1.0 %) were Rashieda tribe (p value = 0.03). Conclusion: The prevalence of Plasmodium vivax malaria was low in our study area comparing with the neighbor countries like Ethiopia. More researches can be performed in this area where Plasmodium falciparum is predominant.

Keywords

References

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Article

Impact of Environmental Conditions on the Germination Time of Penicillium chrysogenum

1Université Péléforo Gon Coulibaly de Korhogo, BP 1328 Korhogo, Côte d’Ivoire

2AgroSup Dijon, Université de Bourgogne, 21000 Dijon, France


American Journal of Microbiological Research. 2016, 4(6), 168-171
doi: 10.12691/ajmr-4-6-2
Copyright © 2016 Science and Education Publishing

Cite this paper:
Sidjè Paule Marina Nanguy, Philippe Dantigny. Impact of Environmental Conditions on the Germination Time of Penicillium chrysogenum. American Journal of Microbiological Research. 2016; 4(6):168-171. doi: 10.12691/ajmr-4-6-2.

Correspondence to: Sidjè  Paule Marina Nanguy, Université Péléforo Gon Coulibaly de Korhogo, BP 1328 Korhogo, Côte d’Ivoire. Email: snanguy@yahoo.fr

Abstract

In predictive mycology, most of the studies have been concerned with the influence of some environmental factors on fungal growth and production of mycotoxins at steady-state. However, germination should be the main step to be focused on, because a product is spoiled shortly after fungal conidia had germinated. In most of the studies dedicated to fungi, germination experiments were carried out immediately after spores were produced and harvested. Very few studies were concerned with the effect of storage conditions on the germination of fungal spores. The aim of this study is to show the impact of relative humidity, time of storage and temperature on germination time for the mould free shelf-life of food products determination. The effects of relative humidity, RH, time of storage, t and temperature, T on τ were assessed according to a Doehlert design in the range, 20-100%, 2-28 days and 5-25°C, respectively. The paramount influence of the relative humidity during storage on the germination time of the conidia of Penicillium chrysogenum was highlighted. An increase of the germination time was shown at reduced RH's, for increased periods of time and at lower temperatures. We can also observe a germination time, more important for conidia stored compared to fresh conidia obtained in the laboratory. The key factor was relative humidity, but time may be also of paramount importance for storage periods that exceed many weeks.

Keywords

References

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