American Journal of Microbiological Research

ISSN (Print): 2328-4129

ISSN (Online): 2328-4137

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Website: http://www.sciepub.com/journal/AJMR

   

Article

Exopolysaccharide (EPS) Production by Exiguobacterium aurantiacum isolated from Marchica Lagoon Ecosystem in Morocco

1Laboratory of Microbiology, Pharmacology, Biotechnology and Environment, University Hassan II, Faculty of Sciences Ain-Chock, route El Jadida, B.P. 5366, Casablanca, Morocco


American Journal of Microbiological Research. 2016, 4(5), 147-152
doi: 10.12691/ajmr-4-5-4
Copyright © 2016 Science and Education Publishing

Cite this paper:
Y. D. Dah Dossounon, K. Lee, K. Belghmi, F. Benzha, M. Blaghen. Exopolysaccharide (EPS) Production by Exiguobacterium aurantiacum isolated from Marchica Lagoon Ecosystem in Morocco. American Journal of Microbiological Research. 2016; 4(5):147-152. doi: 10.12691/ajmr-4-5-4.

Correspondence to: Y.  D. Dah Dossounon, Laboratory of Microbiology, Pharmacology, Biotechnology and Environment, University Hassan II, Faculty of Sciences Ain-Chock, route El Jadida, B.P. 5366, Casablanca, Morocco. Email: djolounivcasa@gmail.com

Abstract

Exiguobacterium aurantiacum, a member of the Bacilli class, has the ability to synthesize and secrete exopolysaccharides. The strain, isolated from Marchica lagoon in Morocco, produced exopolysaccharide (EPS), mainly during its exponential growth phase but also to a lesser extent during the stationary phase. The optimum pH and temperature for growth and exopolysaccharide (EPS) production were 8 and 37°C respectively, the dry weight of the exopolysaccharides products and biomass was found to be 259. 05 ± 1.48 mg/100ml and 150.25 ± 0. 35 mg/100ml respectively. The partially purified exopolysaccharide (EPS) samples were chemically analyzed. The results showed that the temperature and pH have no effect on the protein amount produced by E. aurantiacum while the carbohydrate amount varied. The functional groups in the partially purified exopolysaccharides were determined by the FT-IR. Because of its ability to produce large quantities of exopolysaccharides; this bacterium may prove to be an excellent model species for the development of biotechnology products.

Keywords

References

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Article

Systemic Resistance Induction of Tomato Plants against ToMV Virus by Surfactin Produced from Bacillus subtilis BMG02

1Genetics and Cytology Department , Genetic Engineering and Biotechnology Division, National Research Centre (Affiliation ID : 60014618), Dokki, Giza, Egypt

2Plant Pathology, Botany Departement, Faculty of Agriculture, Minoufia University

3Agricultural Microbiology and Biotechnology, Botany Departement, Faculty of Agriculture, Minoufia University, Shibin El-Kom, Egypt


American Journal of Microbiological Research. 2016, 4(5), 153-158
doi: 10.12691/ajmr-4-5-5
Copyright © 2016 Science and Education Publishing

Cite this paper:
Walaa Hussein, Hosam Awad, Sameh Fahim. Systemic Resistance Induction of Tomato Plants against ToMV Virus by Surfactin Produced from Bacillus subtilis BMG02. American Journal of Microbiological Research. 2016; 4(5):153-158. doi: 10.12691/ajmr-4-5-5.

Correspondence to: Sameh  Fahim, Agricultural Microbiology and Biotechnology, Botany Departement, Faculty of Agriculture, Minoufia University, Shibin El-Kom, Egypt. Email: sameh_fahim@hotmail.com

Abstract

Tomato mosaic virus (ToMV) is a major disease of tomato (Lycopersicon esculentum) which can strongly reduce tomato yields. Different efforts have been made to control tomato virus infection. Among these efforts is the use of cyclic lipopeptides. The surfactin produced by Bacillus subtilis BMG02 single overproducer strain strongly reduced (ToMV) virus symptoms and was responsible for the elicitation of Induced Systemic Resistance (ISR) in tomato. Treatment with 1000 mg surfactin used as critical antivirial concentration showed a strong symptoms reduction with complete negative ELISA results. The infected seedlings showed the ideal severe symptoms with fully positive ELISA reaction, while both healthy and infected seedlings treated with surfactin showed the same effect of strong symptoms reduction with complete negative ELISA results thus confirming the antivirial activities of surfactin. The hydrogen peroxide (H2O2), salicylic acid (SA) and jasmonic acid (JA) pathways have been proven to be involved in the tomato plant defense responses by surfactin treatment, whereas relative gene expression of phenylalanine ammonia lyase (PAL) and β 1,3- glucanase 2 (BGL2) involved in SA and JA pathways, respectively increased in treated seedlings compared to infected ones. The healthy seedlings showed very low H2O2 levels compared to infected tomato seedlings. Moreover, high levels of H2O2 were detected in treated seedlings with the same behaviour BGL2 and PAL expression levels due to the relationship between the ISR involved substrates.

Keywords

References

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Article

In Vitro Antibacterial Activity of Some Natural and Trade Iraqi Honey against MRSA Staphylococcus Heamolyticus Isolated from Some Burned Patients in Misan City

1College of Science, Misan University, Iraq

2College of Basic Education, Misan University, Iraq


American Journal of Microbiological Research. 2016, 4(5), 159-163
doi: 10.12691/ajmr-4-5-6
Copyright © 2016 Science and Education Publishing

Cite this paper:
Mohammed A. Abd Ali, Sailh M. Kidem, Ali A. Fadhil, Nusaybah KH.Saddam. In Vitro Antibacterial Activity of Some Natural and Trade Iraqi Honey against MRSA Staphylococcus Heamolyticus Isolated from Some Burned Patients in Misan City. American Journal of Microbiological Research. 2016; 4(5):159-163. doi: 10.12691/ajmr-4-5-6.

Correspondence to: Mohammed  A. Abd Ali, College of Science, Misan University, Iraq. Email: waleed.abas22@yahoo.com

Abstract

The study is aimed to evaluate the antibacterial activity of both natural Iraqi honeys (Sidr, Eucalyptus) and trade honey sample against MRSA Staphylococcus heamolyticus isolated from burned patients in alsader hospital-misan city then were analyzed between (October to December in 2015). The samples were taken in order to determine the bacterial profile and antibiotic susceptibility. The isolates of Staphylococcus heamolyticus were tested against 5 different antibiotics by a disk diffusion method 100% of the Staphylococcus heamolyticus isolates were resistant to the Optochin, Lincomycin, Ampicillin, Amphotercin, while the inhibitory effect of Amikacin antibiotic were (24mm) on MRSA Staphylococcus heamolyticus. Total antibacterial activity was evaluated by measuring the clear zone around the well. Honey samples were tested in different concentration (75%, 50 %, 25%, 10% and 100%). However, the results showed the absences of inhibitory zone to both natural and trade honeys type on MRSA Staphylococcus heamolyticus tested bacteria after dilution of(25% Sidr honey sample, 50% Eucalyptus honey sample, 50% Trade honey sample), while Sidr honey sample have more effective in 75%v/v concentration against MRSA Staphylococcus heamolyticus tested bacteria. The minimum inhibitory concentration (MIC) of the Sidr honey sample were also determined. Finally cytotoxicity evaluating toward human RBC, the results revealed the Iraqi honeys sample have not any cytotoxicity in all concentration. The good antimicrobial potency of sidr and Eucalyptus Iraqi honeys could potentially be used as therapeutic against MRSA Staphylococcus heamolyticus as an alternative to the costly antibiotics.

Keywords

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