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American Journal of Medical and Biological Research

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ISSN (Online): 2328-4099

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Article

HIV/AIDS and Coronary Heart Disease on a Collision Course? Review of Zimbabwe

1Institute of Clinical Medicine, University in Oslo, Oslo University Hospital, P.O. Box 1171, Blindern, 0318 Oslo, Norway

2University of Zimbabwe, College of Health Sciences, Department of Medical Laboratory Sciences, P. O. Box AV 178, Avondale, Harare, Zimbabwe

3Department of Infectious Diseases, Oslo University Hospital, P.O. Box 4950, Nydalen, 0424, Oslo, Norway

4Chinhoyi University of Technology, PO Box 7724, Chinhoyi, Zimbabwe


American Journal of Medical and Biological Research. 2016, 4(2), 26-32
doi: 10.12691/ajmbr-4-2-3
Copyright © 2016 Science and Education Publishing

Cite this paper:
Danai Tavonga Zhou, Olav Oktedalen, Tawanda Chisango, Babill Stray-Pedersen. HIV/AIDS and Coronary Heart Disease on a Collision Course? Review of Zimbabwe. American Journal of Medical and Biological Research. 2016; 4(2):26-32. doi: 10.12691/ajmbr-4-2-3.

Correspondence to: Danai  Tavonga Zhou, Institute of Clinical Medicine, University in Oslo, Oslo University Hospital, P.O. Box 1171, Blindern, 0318 Oslo, Norway. Email: d.t.zhou@medisin.uio.no

Abstract

There are very few published manuscripts on coronary heart disease (CHD) from Sub-Saharan Africa (SSA) and Zimbabwe. In fact the few published papers on the subject suggest that CHD is rare in SSA and in Zimbabwean individuals both before HIV and in the era of HIV/AIDS and antiretroviral therapy (ART). However a look at recent literature and data from publications by both the World Health Organization (WHO) and Zimbabwe Ministry of Health and Child Care point towards an epidemiological transition, as CHD is now one of the top causes of death in Zimbabwe. This may be due to the fact that CHD is a complex inflammatory disease involving smoking, obesity, diabetes, atherogenic lipid levels, cytokines and other inflammatory markers such as C reactive protein (CRP) and myeloperoxidase (MPO) and many of these factors have consistently been linked to changing lifestyle as populations move into urban settings. On the other hand, atherogenic lipids and elevated inflammatory markers are more common in HIV infected individuals due to the virus and ART. Hence it is likely that the burden of non-communicable diseases (NCDs) and CHD will increase over time as a result of both urbanization in the general population and a high HIV disease burden in Zimbabwe, pointing towards a collision of HIV and CHD in future.

Keywords

References

[1]  World Health Organization (2015) Deaths from coronary heart disease, Available @ http://www.who.int/cardiovascular_diseases/en/cvd_atlas_14_deathHD.pdf.
 
[2]  Beaglehole R, Bonita R (2008) Global public health: A scorecard. Lancet; 372(9654):1988-1996.
 
[3]  McKeown RE (2009) The Epidemiologic Transition: Changing Patterns of Mortality and Population Dynamics. American Journal of Lifestyle Medicine;3(1 Suppl):19S-26S.
 
[4]  World Health Organisation (2010) Global status report on non-communicable diseases 2010, Available @ http://www.who.int/nmh/publications/ncd_report_full_en.pdf.
 
[5]  Zimbabwe Ministry of Health and Child Care (2015) Top 50 causes of death, Available @ http://www.mohcc.gov.zw/country-health-profile/14-sample-data-articles/105-coming-soon-3.
 
Show More References
[6]  World Health Organization (2015) Non communicable Diseases (NCD) Country Profiles 2014, Available @ http://www.who.int/nmh/countries/zwe_en.pdf.
 
[7]  Mbewu A, Mbanya J-C (2006) Cardiovascular Disease in Disease and Mortality in Sub-Saharan Africa. In CVD in Africa, Jamison DT, Feachem RG, Makgoba MW, et al., editors, 2nd edition. Washington (DC): World Bank.
 
[8]  BeLue R, Okoror TA, Iwelunmor J, Taylor KD, Degboe AN, Agyemang C, Ogedegbe G (2009) An overview of cardiovascular risk factor burden in sub-Saharan African countries: a socio-cultural perspective, Globalization and Health, 5:10.
 
[9]  Zimbabwe Country Report January-December 2014, (2015) Global AIDS Response Progress Report 2015 Follow-Up to the 2011 Political Declaration on HIV/AIDS: Intensifying our Efforts to Eliminate HIV/AIDS, Available http://www.unaids.org/sites/default/files/country/documents/ZWE_narrative_report_2015.pdf.
 
[10]  Feeney ER, Mallon PWG (2011) HIV and HAART-Associated Dyslipidemia, The Open Cardiovascular Medicine Journal, 5: 49-63.
 
[11]  Karari E, Manuthu EM, Lule GN (2008) Prevalence of dyslipidemia and dysglycaemia in HIV infected patients, East African Medical Journal, 85:10-7.
 
[12]  Yusuf S, on behalf of the PURE Investigators, (2013) PURE: Contrasting associations between risk factor burden, CVD incidence and mortality in high, middle and low income countries, Presentation at European Society of Cardiology Congress.
 
[13]  World Health Organization (2014) Zimbabwe: Coronary Heart Disease, Available @ http://www.worldlifeexpectancy.com/zimbabwe-coronary-heart-disease.
 
[14]  Zimbabwe Health and Demographics Survey 2010-2011. Available @ http://microdata.worldbank.org/index.php/catalog/1532.
 
[15]  Zimbabwe Map (2016) Available @ https://encrypted-tbn0.gstatic.com/images? q=tbn:ANd9GcTfgI8RwZqKpffv8P6oE4TkugD7hjCCVhAiTPBWypCm4NgVLKxT.
 
[16]  CIA-Factbook, Zimbabwe – Economy 2016 (2016) Available @ http://www.theodora.com/wfbcurrent/zimbabwe/zimbabwe_economy.html.
 
[17]  Trading Economics (2016) Zimbabwe Unemployment Rate, Available @ http://www.tradingeconomics.com/zimbabwe/unemployment-rate.
 
[18]  Zimbabwe National Statistics Agency (2016) Unemployment rate, Available @ http://www.zimstat.co.zw/.
 
[19]  Brenner, M. Harvey (1979) Influence of the Social Environment on Psychology: The Historical Perspective. In Stress and Mental Disorder, Barrett E Ed, Raven Press. New York.
 
[20]  World Bank (2016) Zimbabwe Population Growth Rate Chart, Available @ www.multpl.com/zimbabwe-population-growth-rate.
 
[21]  Index Mundi (2016) Zimbabwe Death Rates, Available @ http://www.indexmundi.com/g/g.aspx?c=zi&v=26.
 
[22]  Tibaijuka AK, UN Special Envoy on Human Settlements Issues in Zimbabwe (2005) Report of the Fact-Finding Mission to Zimbabwe to assess the Scope and Impact of Operation Murambatsvina.
 
[23]  Centers for Disease Control (1981) Pneumocystis pneumonia-Los Angeles. Morbidity and Mortality Weekly Report; 30:250-252.
 
[24]  World Bank (2014) Graphs of life expectancy at birth for some Sub-Saharan countries, Available @ https://www.pinterest.com/pin/189854940514689842/.
 
[25]  UNAIDS Report on the Global AIDS Epidemic (2012) Available @http://www.unaids.org/sites/default/files/en/media/unaids/contentassets/documents/epidemiology/2012/.
 
[26]  AIDS and TB Program/Zimbabwe Ministry of Health and Child Care/National AIDS Council (2013) Zimbabwe National HIV Estimates, Available @ http://www.nac.org.zw/sites/default/files/Zimbabwe-National-HIV-and%20AIDS-2013%20Estimates.doc.
 
[27]  Mahomva A, Greby S, Dube S, Mugurungi O, Hargrove J, Rosen D, Dehne K-L, Gregson S, St Louis M, Hader S (2006) HIV prevalence and trends from data in Zimbabwe, 1997–2004. Sexually Transmitted Infections. 2006; 82(Suppl 1): i42-i47.
 
[28]  National AIDS Council (2013) HIV and AIDS Statistics, Available @ http://www.nac.org.zw/category/tags/hiv-and-aids-statistics.
 
[29]  CDC National Prevention Information Network (2003) Determinants of survival following HIV-1 sero-conversion after the introduction of HAART, The Lancet Vol. 362, 1267-1274, Available @ http://www.thebody.com/content/art29375.html.
 
[30]  BBC News (1999) World: Africa AIDS drug trade dispute ends, Available @ http://news.bbc.co.uk/2/hi/africa/450942.stm.
 
[31]  Zimbabwe Ministry of Health and Child Care (2013) Guidelines for Antiretroviral Therapy for the Prevention and Treatment of HIV in Zimbabwe National Medicine and Therapeutics Policy Advisory Committee (NMTPAC) and the AIDS and TB Directorate.
 
[32]  The Henry J Kaiser Family Foundation (2015) The Global HIV/AIDS Epidemic, Available @ff.org/global-health-policy/fact-sheet/the-global-hivaids-epidemic/.
 
[33]  Chastain DB, Henderson H, Stover KR (2015) Epidemiology and Management of Antiretroviral-Associated Cardiovascular Disease, Open AIDS J; 9: 23-37.
 
[34]  Sliwa K, Carrington MJ, Becker A, Thienemann F, Ntsekhe M, Stewart S (2011) Contribution of the human immunodeficiency virus/acquired immunodeficiency syndrome epidemic to de novo presentations of heart disease in the Heart of Soweto Study cohort.
 
[35]  Barbaro G, Rafael da Silva EF (2009) Cardiovascular complications in the acquired immunodeficiency syndrome, Rev Assoc Med Bras; 55:5.
 
Show Less References

Article

Farmer: Agrochemical Use and Associated Risk Factors in Fadan Daji District of Kaura LGA, Kaduna State, Nigeria

1Department of Community Medicine, College Medicine and Health Sciences, Bingham University, Jos, Nigeria

2Department of Medical Microbiology, College Medicine and Health Sciences, Bingham University, Jos, Nigeria

3Department of Pharmacology and Therapeutics, College Medicine and Health Sciences, Bingham University, Jos, Nigeria

4Department of Biology and Center for Cancer Research, Tuskegee University, Tuskegee, Alabama, USA

5Department of Haematology, College Medicine and Health Sciences, Bingham University, Jos, Nigeria

6Department of Chemical Pathology, College Medicine and Health Sciences, Bingham University, Jos, Nigeria

7Department of Medicine and Surgery, College Medicine and Health Sciences, Bingham University, Jos, Nigeria

8Department of Community Medicine, College Medicine, University of Abuja, Nigeria


American Journal of Medical and Biological Research. 2016, 4(3), 33-41
doi: 10.12691/ajmbr-4-3-1
Copyright © 2016 Science and Education Publishing

Cite this paper:
Bassi AP, Ramyil MCS, Ogundeko TO, Abisoye-Ogunniyan A, Builders M, Thliza SMA, Adeniyi GO, Akande Tunji, Ike RO, Ologun DO, Damai C, Pfongkazah D, Adinoyi OA, Dibigbo-Ibeagi Ndudi Mary, Nwankwo B. Farmer: Agrochemical Use and Associated Risk Factors in Fadan Daji District of Kaura LGA, Kaduna State, Nigeria. American Journal of Medical and Biological Research. 2016; 4(3):33-41. doi: 10.12691/ajmbr-4-3-1.

Correspondence to: Ogundeko  TO, Department of Pharmacology and Therapeutics, College Medicine and Health Sciences, Bingham University, Jos, Nigeria. Email: tim_ogundeko@yahoo.com

Abstract

Background: Occupational poisoning via the use of agrochemicals is common in developing countries. This is because grass-root farmers, who are the majority, are poor, illiterate and are undertrained on ethics surrounding its use. This is a cross sectional descriptive study carried out in Fadan-Daji (FD) district of Kagoro Chiefdom, Kaura LGA, Kaduna state, Nigeria. Methods: Three villages - Kodwak, Uzha-Tuyit and Zankam were randomly selected in the district. A sample size of 250 farmers was obtained using a p-value of 82%, while the selected farmers completed structured questionnaires. Results: Physical, chemical, biological, mechanical and psychosocial events occurred at the same time in the FD Farming community. Thus, clients present with multiple finding or symptoms. In this study most farmers experienced chest pain/tightness, cough, dizziness, reddening of the eyes; sneezing and rheum more often following the use on their farms. This study further revealed that farmers in the studied communities (31.6%) resorted to self-medication ranging from analgesics, to over the counter antihistamines, whilst 23.6% reported that they ingest milk as antidote. 32.4% did nothing about it while only 12.4% of the respondents visited a health facility. 54.4% of FD farmers used no form of personal protective devices (PPDs). Conclusion: Our findings revealed that the root problem faced with the FD like the average farmer of the developing world is lack of relevant education in terms of ethical use and disposal of agrochemicals which has made a significant number of FD farmers more susceptible to the agrochemical side effects resulting from its use. Such a community located in the heart of Nigeria also being a food basket needs prompt attention in terms of improved western/farmer education and health facilities.

Keywords

References

[1]  Okoloko, O., (2006). African Green Revolution Paper Delivered at African Inorganic fertilizer Summit, (June 9-13, 2006), Abuja, Nigeria. Retrieved from: http://www.africainorganic.fertilizersummit.org (Accessed on: December 10, 2015).
 
[2]  Avav, T. and Oluwatayo, J. I. (2006). Environmental and Health Impact of Pesticides. Jolytta Publications, Makurdi. . Ayansina ADV, Ogunshe AAO, Fagade OE (2003). Environment Impact Assessment and Microbiologist: An overview. Proc. Of 11th annual national conf. of Environment and Behaviour Association of Nig. (EBAN), pp. 26-27.
 
[3]  Ayoola, G. B. (1990). The Marketing of Agricultural Pesticides in Nigeria. National Workshop on the Pesticides Industry in Nigeria. University of Ibadan, 6-8 February 1990.Publication No. 11.
 
[4]  Christin MS, Me´nard L, Gendron AD, Ruby S, Cyr D, Marcogliese DJ, Rollins- Smith L, Fournier M. 2004. Effects of agricultural pesticides on the immune system of Xenopus laevis and Rana pipiens. Aquatic Toxicology, 67, 33-43.
 
[5]  Gu X, Tian S. 2005. Pesticide and cancer. World’s Scientific Technology and Develoment, 27(2), 47-52.
 
Show More References
[6]  Galloway T, Handy R. 2007. Immunotoxicity of organophosphorous pesticides. Ecotoxicology, 12(1-4), 345-363.
 
[7]  Tuc VP, Wangsuphachart V, Tasanapradit P, Fungladda W, Van Trong P, Nhung NT. 2007. Impacts of pesticide use on semen characteristics among rice farmers in Kienxuong District, Thaibinh Province, Vietnam. Southeast Asian Journal of Tropical Medicine and Public Health, 3, 569-575.
 
[8]  Hoppin JA, Umbach DM, London SJ, Alavanja MCR, Sandler DP. 2002. Chemical predictors of wheeze among farmer pesticide applicators in the agricultural health study. American Journal of Respiratory and Critical Care Medicine, 5, 683-689.
 
[9]  Ide C (2008). Pastoral Care, Safety Health Practitioner Magazine, Nov 2008 United Media. International Inorganic Fertilizer Development Center (IFDC), (1996). Africa Inorganic fertilizer Situation, IFDC November.
 
[10]  Bohmont BL. 1990. The standard pesticide user’s guide. Upper Saddle River (NJ): Prentice Hall.
 
[11]  Simon-Sylvestre G, Fournier JC (1979). Effects of Pesticides on Soil Micro Flora. Adv. Agron. 31: 1-92
 
[12]  Friedrich T (1996). Agricultural Pesticide Application. FAO Agricultural Engineering Branch AGSE, FAO Rome.
 
[13]  Kalia A, Gupta RP. 2004. Disruption of soil foodweb by pesticides. Ind J Ecol. 31(2):85-92.
 
[14]  Blair A, Zahm SH. Cancer among farmers. Occup Med1991;6:335-54.
 
[15]  Jors E. 2004. Acute Pesticide Poisonings Among Small-Scale Farmers in La Paz County, Bolivia [master’s thesis]. Copenhagen: University of Copenhagen
 
[16]  Lawal BO,Torimiro DO, Banjo AD, Joda AO: Operational Habits and Health Hazards Associated With Pesticide Usage by Cocoa Farmers in Nigeria: Lessons for ExtensionWork. J. Hum. Ecol., 17(3): 191-195 (2005).
 
[17]  Meijden G, van der (1998). Pesticide Application Techniques in West Africa. A study by the Agricultural Engineering Branch of FAO through the FAO Regional Office for Africa. 17pp.
 
[18]  Ajayi, O. Akinnifesi, F. and Sileshi, G. (2011) Human health and occupational exposure to pesticides among smallholder farmers in cotton zones of Côte d'Ivoire. Health, 3, 631-637.
 
[19]  Mokhele T. Potential health effects of pesticide use on farmworkers in Lesotho. S Afr J Sci. 2011;107(7/8), Art. #509, 7 pages.
 
[20]  Ogunjimi SI. Farinde AJ: Farmers’ Knowledge Level of Precautionary Measures in Agro -Chemicals Usage on Cocoa Production in Osun and Edo States, Nigeria International Journal of Agriculture and Forestry 2012, 2(4): 186-194.
 
[21]  Buczynska A, Szadkowska-Stanczyk I. 2005. Identification of health hazards to rural population living near pesticide dump sites in Poland. International Journal of Occupational Medicine and Environmental Health, 18, 331-339.
 
[22]  Leyk S, Binder CR, Nuckols JR. 2009. Spatial modeling of personalized exposure dynamics: The case of pesticide use in small-scale agricultural production landscapes of the developing world. International Journal of Health Geographics, 8(17), 1-16.
 
[23]  Elfvendahl S, Mihale M, Kishimba MA, Kylin H. 2004. Pesticide pollution remains severe after cleanup of a stockpile of obsolete pesticides at Vikuge, Tanzania. Ambio, 33, 503-508.
 
[24]  International Agency for Research on Cancer. IARC Monographs on the Evaluation of Carcinogenic Risks to Humans; some N-nitroso compounds. In: IARC Monogr Eval Carcinog Risk Chem.Man. Vol 17 Lyon, France: IARC, 1978; 1-349.
 
[25]  Magee PN. The experimental basis for the role of nitroso compounds in human cancer. Cancer Surv1989; 8: 207-39.
 
[26]  Eichholzer M, Gutzwiller F. Dietary nitrates, nitrites, and N-nitroso compounds and cancer risk: a review of the epidemiologic evidence. Nutr Rev1998; 56:95-105.
 
[27]  Musicco M, Sant M, Molinari S, et al. A case-control study of brain gliomas and occupational exposure to chemical carcinogens: the risk to farmers. Am J Epidemiol1988; 128: 778-85.
 
[28]  Otunaiya, O.A, Okuneye P.A., and Aihonsu., J.O.Y.: Pattern of Inorganic Fertilizer use among Food Crop; Farmers in Ogun State, Nigeria Asian Journal of Agricultural Sciences 4(1): 26-31, 2012
 
[29]  Ahemba, T., Inorganic Fertilizer Plant Restart after 10 Years. 2009 Retrieved from: http//www. Online Nigeria.com.
 
[30]  Govinda Bhandari, “An Overview of Agrochemicals and Their Effects on Environment in Nepal.” Applied Ecology and Environmental Sciences, vol. 2, no. 2 (2014): 66-73.
 
[31]  “Post Offices- with map of LGA”. NIPOST. Retrieved 2009-10-20.
 
[32]  Fisher RA. The logic of inductive inference (with discussion). Journal of Royal Statistical Society. 1935;98:39-82.
 
[33]  Toyin Samuel Olowogbon, Segun Bamidele Fakayode, Ademola John Jolaiya, Adebola Omolara Oke. Agrochemicals, Health, Safety, Implications, Nigeria and Small Scale Farmers Journal of Sustainable Development in Africa (Volume 15, No.1, 2013) ISSN: 1520-5509 Clarion University of Pennsylvania, Clarion, Pennsylvania.
 
[34]  Kaliyaperuma Karunamoorthi, Abraham Yirgalem :Insecticide Risk Indicators and Occupational Insecticidal Poisoning in Indoor Residual Spraying. Health Scope. 2013 February; 1(4): 165-172.
 
[35]  Yaser Issa, Farid Abu Sham’a, Khaldoun Nijem, Espen Bjertness, Petter Kristensen: Pesticide use and opportunities of exposure among farmers and their families: cross-sectional studies 1998-2006 from Hebron governorate,occupied Palestinian territory. Environ Health. 2010;9:63.pp1-10.
 
[36]  Tijani AA. 2006. Pesticide use practices and safety issues: the case of cocoa farmers in Ondo State, Nigeria. J Hum Ecol. 2006; 19: 183-190.
 
[37]  Faith Eyayo: Evaluation of Occupational Health Hazards among Oil Industry Workers: A Case Study of Refinery Workers. IOSR Journal of Environmental Science, Toxicology and Food Technology (IOSR-JESTFT) e-ISSN: 2319-2402, p-ISSN: 2319-2399.Volume 8, Issue 12 Ver. I (Dec. 2014), PP 22-53.
 
[38]  Wise GEEK. http:www.wisegeek.com/what-are-occupational-hazards.htm(cited October 2013).
 
[39]  Business Dictionary. http://www.businessdictionary.com (cited October 2013).
 
[40]  Lebov, J. F. et al. Pesticide exposure and end-stage renal disease risk among wives of pesticide applicators in the Agricultural Health Study. Environ. Res. 143, 198-210 (2015).
 
[41]  Mamane A, Baldi I, Tessier JF, Raherison C, Bouvier G. Occupational exposure to pesticides and respiratory health. Eur Respir Rev. 2015 Jun;24(136):306-19.
 
[42]  Beseler CL, Stallones L. Pesticide poisoning and respiratory disorders in Colorado farm residents. J Agric Saf Health. 2009 Oct; 15(4): 327-34.
 
[43]  Collotta M, Bertazzi PA, Bollati V. Epigenetics and pesticides. Toxicology. 2013 May 10;307: 35-41.
 
[44]  Baccarelli A, Bollati V. Epigenetics and environmental chemicals. Curr Opin Pediatr. 2009 Apr;21(2):243-51.
 
[45]  Antwi, S. O. et al. Exposure to environmental chemicals and heavy metals, and risk of pancreatic cancer. Cancer Causes {&} Control 26, 1583-1591 (2015).
 
[46]  Andreotti G, Silverman DT: Occupational risk factors and pancreatic cancer: a review of recent findings. Mol. Carcinog. 51, 98-108 (2012).
 
[47]  Silva, João F.S., Inês E. Mattos, Laércio L. Luz, et al. “Exposure to pesticides and prostate cancer: systematic review of the literature” Reviews on Environmental Health, 0.0 (2016)
 
[48]  Lewis-Mikhael AM, Bueno-Cavanillas A, Ofir Guiron T, Olmedo-Requena R, Delgado-Rodríguez M, Jiménez-Moleón JJ. Occupational exposure to pesticides and prostate cancer: a systematic review and meta-analysis. Occup Environ Med. 2016 Feb; 73(2): 134-44.
 
[49]  Van Maele-Fabry G, Duhayon S, Mertens C, Lison D. Risk of leukaemia among pesticide manufacturing workers: a review and meta-analysis of cohort studies. Environ Res. 2008 Jan; 106(1):121-37.
 
[50]  Van Maele-Fabry G, Duhayon S, Lison D. A systematic review of myeloid leukemias and occupational pesticide exposure. Cancer Causes Control. 2007 Jun; 18(5):457-78.
 
[51]  Van Maele-Fabry G, Lantin AC, Hoet P, Lison D. Childhood leukaemia and parental occupational exposure to pesticides: a systematic review and meta-analysis. Cancer Causes Control. 2010 Jun; 21(6):787-809.
 
[52]  Gómez-Barroso D, García-Pérez J, López-Abente G, Tamayo-Uria I, Morales-Piga A, Pardo Romaguera E, Ramis R. Agricultural crop exposure and risk of childhood cancer: new findings from a case-control study in Spain. Int J Health Geogr. 2016 May 31;15(1):18.
 
[53]  Fenga C. Occupational exposure and risk of breast cancer. Biomed Rep. 2016 Mar;4(3):282-292. Epub 2016 Jan 21.
 
[54]  Wolff MS, Collman GW, Barrett JC, Huff J. Breast cancer and environmental risk factors: epidemiological and experimental findings. Annu Rev Pharmacol Toxicol. 1996; 36:573-96.
 
[55]  Brody JG, Rudel RA. Environmental pollutants and breast cancer. Environ Health Perspect. 2003 Jun;111(8):1007-19.
 
[56]  World Health Organization: Recommended Health based occupational exposure limits for selected vegetable dusts (Report of a study group). WHO Technical report series 1983; 684:35-49.
 
[57]  Farmworker justice: Immigration and labour health initiatives, occupational health and safety. 1126 16th St NW # 270, Washington, DC, 20036 | (202) 293-5420. Cited online 8th June,
 
[58]  Huang Y, Liu L, Pei E. 2008. Vegetative pesticide residue and application behavior in Beijing. Chinese Journal of Food Hygiene, 20, 319-321.
 
[59]  Xu Y. 2004. Survey of improper pesticide application in rice production. Jiangxi Agricultural Technology, 11, 27-28.
 
[60]  Dongmei Z. 2006. Development of bio-pesticide industry in China. Unpublished PhD thesis, Fujian University of Agriculture and Forestry, Fujian, China.
 
Show Less References

Article

Role of Some Metal Ions on Steady–state Kinetics of Engineered Wild–type and Manganese (II) Binding Site Mutants of Recombinant Phlebia radiata Manganese Peroxidase 3 (rPr-MnP3)

1Department of Biological Sciences, Akwa Ibom State University, P.M.B. 1167, Uyo, Akwa Ibom State, Nigeria

2Department of Chemistry, Akwa Ibom State University, P.M.B. 1167, Uyo, Akwa Ibom State, Nigeria

3Research and Development Unit, Akwa Ibom State University, P.M.B. 1167, Uyo, Akwa Ibom State, Nigeria

4Department of Biochemistry, University of Uyo, P. M. B. 1017, Uyo, Akwa Ibom State, Nigeria


American Journal of Medical and Biological Research. 2016, 4(3), 42-52
doi: 10.12691/ajmbr-4-3-2
Copyright © 2016 Science and Education Publishing

Cite this paper:
Usenobong F. Ufot, Aniefiok E. Ite, Idorenyin H. Usoh, Monday I. Akpanabiatu. Role of Some Metal Ions on Steady–state Kinetics of Engineered Wild–type and Manganese (II) Binding Site Mutants of Recombinant Phlebia radiata Manganese Peroxidase 3 (rPr-MnP3). American Journal of Medical and Biological Research. 2016; 4(3):42-52. doi: 10.12691/ajmbr-4-3-2.

Correspondence to: Usenobong  F. Ufot, Department of Biological Sciences, Akwa Ibom State University, P.M.B. 1167, Uyo, Akwa Ibom State, Nigeria. Email: usen_mboso@yahoo.com

Abstract

This study investigated the steady-state kinetics of engineered wild-type and manganese (II) binding site mutants of recombinant Phlebia radiata manganese peroxidase 3(rPr-MnP3). The effect (activation or inhibition) of some metal ions (Co2+, Zn2+ Cu2+ and Na+) on the activity of rPr-MnP3 enzymes was also studied. The results obtained showed that the rPr-MnP3 mutants in which the metal binding functionality has been largely lost have been created. Na+ (mono-valent ion) and Co2+showed similar characteristics by exhibiting stimulatory effects on the activity of wild-type rPr-MnP3. However, Cu2+ and Zn2+ had mixed inhibitory effects on wild-type and mutants (E40H, E44H, E40H/E44H). It was observed that Cu2+ was by far the strongest inhibitor of engineered rPr-MnP3 enzymes while Co2+ exhibited a non-competitive inhibitory effect on the double mutant (E40H/E44H) and D186H activities. In addition, Zn2+ and Cu2+also had non-competitive inhibitory effect on D186H mutant enzyme activity. The results obtained further showed that the competitive inhibitory effect of Cu2+observed in other rPr-MnP3 enzymes is largely removed in D186H mutant enzyme. Generally, histidine substitution retained a strong selectivity for Cu2+ as competitive inhibitor. Zn2+ being generally non-competitive suggest involvement of sites other than the Mn (II) binding site. This study showed that rPr-MnP3 enzymes function with alternate ligands in the Mn2+ binding site and does not have absolute obligate requirement for all carboxylate ligand set.

Keywords

References

[1]  Hatakka, A. I., and A. K. Uusi-Rauva, “Degradation of 14C-labelled poplar wood lignin by selected white-rot fungi,” European journal of applied microbiology and biotechnology, 17 (4). 235-242, 1983.
 
[2]  Lundell, T., A. Leonowicz, J. Rogalski, and A. Hatakka, “Formation and Action of Lignin-Modifying Enzymes in Cultures of Phlebia radiata Supplemented with Veratric Acid,” Applied and Environmental Microbiology, 56 (9). 2623-2629, 1990.
 
[3]  Vares, T., M. Kalsi, and A. Hatakka, “Lignin Peroxidases, Manganese Peroxidases, and Other Ligninolytic Enzymes Produced by Phlebia radiata during Solid-State Fermentation of Wheat Straw,” Applied and Environmental Microbiology, 61 (10). 3515-3520, 1995.
 
[4]  Hatakka, A., T. Lundell, M. Hofrichter, and P. Maijala, “Manganese Peroxidase and Its Role in the Degradation of Wood Lignin,” Applications of Enzymes to Lignocellulosics, ACS Symposium Series 855, S. D. Mansfield and J. N. Saddler, eds., pp. 230-243: American Chemical Society, 2003.
 
[5]  Niemenmaa, O., A. Uusi-Rauva, and A. Hatakka, “Wood stimulates the demethoxylation of [O14CH3]-labeled lignin model compounds by the white-rot fungi Phanerochaete chrysosporium and Phlebia radiata,” Archives of Microbiology, 185 (4). 307-315, 2006.
 
Show More References
[6]  Hatakka, A., and K. E. Hammel, “Fungal Biodegradation of Lignocelluloses,” Industrial Applications, M. Hofrichter, ed., pp. 319-340, Berlin, Heidelberg: Springer Berlin Heidelberg, 2011.
 
[7]  Marco-Urrea, E., and C. A. Reddy, “Degradation of Chloro-organic Pollutants by White Rot Fungi,” Microbial Degradation of Xenobiotics, N. S. Singh, ed., pp. 31-66, Berlin, Heidelberg: Springer Berlin Heidelberg, 2012.
 
[8]  Lundell, T., “Ligninolytic System of the White-rot Fungus Phlebia radiata: Lignin Model Compound Studies,” Department of Applied Chemistry and Microbiology, University of Helsinki, Helsinki, 1993.
 
[9]  Karhunen, E., A. Kantelinen, and M.-L. Niku-Paavola, “Mn-dependent peroxidase from the lignin-degrading white rot fungus Phlebia radiata,” Archives of Biochemistry and Biophysics, 279 (1). 25-31, 1990.
 
[10]  Moilanen, A. M., T. Lundell, T. Vares, and A. Hatakka, “Manganese and malonate are individual regulators for the production of lignin and manganese peroxidase isozymes and in the degradation of lignin by Phlebia radiata,” Applied Microbiology and Biotechnology, 45 (6). 792-799, 1996.
 
[11]  Hildén, K. S., M. R. Mäkelä, T. K. Hakala, A. Hatakka, and T. Lundell, “Expression on wood, molecular cloning and characterization of three lignin peroxidase (LiP) encoding genes of the white rot fungus Phlebia radiata,” Current Genetics, 49 (2). 97-105, 2005.
 
[12]  Lundell, T. K., M. R. Mäkelä, and K. Hildén, “Lignin-modifying enzymes in filamentous basidiomycetes – ecological, functional and phylogenetic review,” Journal of Basic Microbiology, 50 (1). 5-20, 2010.
 
[13]  Hofrichter, M., R. Ullrich, M. J. Pecyna, C. Liers, and T. Lundell, “New and classic families of secreted fungal heme peroxidases,” Applied Microbiology and Biotechnology, 87 (3). 871-897, 2010.
 
[14]  Glenn, J. K., and M. H. Gold, “Purification and characterization of an extracellular Mn(II)-dependent peroxidase from the lignin-degrading basidiomycete, Phanerochaete chrysosporium,” Archives of Biochemistry and Biophysics, 242 (2). 329-341, 1985.
 
[15]  Paszczyński, A., V.-B. Huynh, and R. Crawford, “Enzymatic activities of an extracellular, manganese-dependent peroxidase from Phanerochaete chrysosporium,” FEMS Microbiology Letters, 29 (1-2). 37-41, 1985.
 
[16]  Hofrichter, M., “Review: lignin conversion by manganese peroxidase (MnP),” Enzyme and Microbial Technology, 30 (4). 454-466, 2002.
 
[17]  Wariishi, H., K. Valli, and M. H. Gold, “Manganese(II) oxidation by manganese peroxidase from the basidiomycete Phanerochaete chrysosporium. Kinetic mechanism and role of chelators,” Journal of Biological Chemistry, 267 (33). 23688-23695, 1992.
 
[18]  Kirk, T. K., and R. L. Farrell, “Enzymatic “Combustion”: The Microbial Degradation of Lignin,” Annual Review of Microbiology, 41 (1). 465-501, 1987.
 
[19]  Kersten, P., and D. Cullen, “Extracellular oxidative systems of the lignin-degrading Basidiomycete Phanerochaete chrysosporium,” Fungal Genetics and Biology, 44 (2). 77-87, 2007.
 
[20]  Buswell, J. A., E. Odier, and T. K. Kirk, “Lignin Biodegradation,” Critical Reviews in Biotechnology, 6 (1). 1-60, 1987.
 
[21]  Martinez, D., L. F. Larrondo, N. Putnam, M. D. S. Gelpke, K. Huang, J. Chapman, K. G. Helfenbein, P. Ramaiya, J. C. Detter, F. Larimer, P. M. Coutinho, B. Henrissat, R. Berka, D. Cullen, and D. Rokhsar, “Genome sequence of the lignocellulose degrading fungus Phanerochaete chrysosporium strain RP78,” Nat Biotech, 22 (6). 695-700, 2004.
 
[22]  Hildén, K., A. T. Martinez, A. Hatakka, and T. Lundell, “The two manganese peroxidases Pr-MnP2 and Pr-MnP3 of Phlebia radiata, a lignin-degrading basidiomycete, are phylogenetically and structurally divergent,” Fungal Genetics and Biology, 42 (5). 403-419, 2005.
 
[23]  Martı́nez, A. T., “Molecular biology and structure-function of lignin-degrading heme peroxidases,” Enzyme and Microbial Technology, 30 (4). 425-444, 2002.
 
[24]  Sundaramoorthy, M., K. Kishi, M. H. Gold, and T. L. Poulos, “Crystal Structures of Substrate Binding Site Mutants of Manganese Peroxidase,” Journal of Biological Chemistry, 272 (28). 17574-17580, 1997.
 
[25]  Kusters-van Someren, M., K. Kishi, T. Lundell, and M. H. Gold, “The Manganese Binding Site of Manganese Peroxidase: Characterization of an Asp179Asn Site-Directed Mutant Protein,” Biochemistry, 34 (33). 10620-10627, 1995.
 
[26]  Kishi, K., M. Kusters-van Someren, M. B. Mayfield, J. Sun, T. M. Loehr, and M. H. Gold, “Characterization of Manganese (II) Binding Site Mutants of Manganese Peroxidase,” Biochemistry, 35 (27). 8986-8994, 1996.
 
[27]  Boucher, L. J., K. Koeber, M. Kotowski, and D. Tille, “Coordination Compounds of Manganese,” Coordination Compounds 7, H. Demmer, M. Kotowski, E. Schleitzer-Rust and D. Tille, eds., pp. 1-2, Berlin, Heidelberg: Springer Berlin Heidelberg, 1989.
 
[28]  Sundaramoorthy, M., K. Kishi, M. H. Gold, and T. L. Poulos, “The crystal structure of manganese peroxidase from Phanerochaete chrysosporium at 2.06-A resolution,” Journal of Biological Chemistry, 269 (52). 32759-32767, 1994.
 
[29]  Sundaramoorthy, M., H. L. Youngs, M. H. Gold, and T. L. Poulos, “High-Resolution Crystal Structure of Manganese Peroxidase:  Substrate and Inhibitor Complexes,” Biochemistry, 44 (17). 6463-6470, 2005.
 
[30]  Gold, M. H., H. L. Youngs, and M. D. Sollewijn Gelpke, “Manganese Peroxidase,” Metal Ions in Biological Systems: Volume 37: Manganese and Its Role in Biological Processes, A. Sigel and H. Sigel, eds., pp. 559-586, New York, USA: CRC Press, 2000.
 
[31]  Kuan, I. C., K. A. Johnson, and M. Tien, “Kinetic analysis of manganese peroxidase. The reaction with manganese complexes,” Journal of Biological Chemistry, 268 (27). 20064-20070, 1993.
 
[32]  Kishi, K., H. Wariishi, L. Marquez, H. B. Dunford, and M. H. Gold, “Mechanism of Manganese Peroxidase Compound II Reduction. Effect of Organic Acid Chelators and pH,” Biochemistry, 33 (29). 8694-8701, 1994.
 
[33]  Paszczyński, A., V.-B. Huynh, and R. Crawford, “Comparison of ligninase-I and peroxidase-M2 from the white-rot fungus Phanerochaete chrysosporium,” Archives of Biochemistry and Biophysics, 244 (2). 750-765, 1986.
 
[34]  Wariishi, H., H. B. Dunford, I. D. MacDonald, and M. H. Gold, “Manganese peroxidase from the lignin-degrading basidiomycete Phanerochaete chrysosporium. Transient state kinetics and reaction mechanism,” Journal of Biological Chemistry, 264 (6). 3335-3340, 1989.
 
[35]  Gregory, D. S., A. C. R. Martin, J. C. Cheetham, and A. R. Rees, “The prediction and characterization of metal binding sites in proteins,” Protein Engineering, 6 (1). 29-35, 1993.
 
[36]  Kennedy, M. L., and B. R. Gibney, “Metalloprotein and redox protein design,” Current Opinion in Structural Biology, 11 (4). 485-490, 2001.
 
[37]  Maneiro, M., W. F. Ruettinger, E. Bourles, G. L. McLendon, and G. C. Dismukes, “Kinetics of proton-coupled electron-transfer reactions to the manganese-oxo “cubane” complexes containing the Mn4O and Mn4O core types,” Proceedings of the National Academy of Sciences, 100 (7). 3707-3712, 2003.
 
[38]  Garcia, J. S., C. S. d. Magalhães, and M. A. Z. Arruda, “Trends in metal-binding and metalloprotein analysis,” Talanta, 69 (1). 1-15, 2006.
 
[39]  Pecoraro, V., and W. Hsieh, “The use of model complexes to elucidate the structure and function of manganese redox enzymes,” Metals in Biological Systems, A. S. a. H. Sigel, ed., pp. 429-504, New York, USA: CRC Press, 2000.
 
[40]  Puglisi, A., G. Tabbı̀, and G. Vecchio, “Bioconjugates of cyclodextrins of manganese salen-type ligand with superoxide dismutase activity,” Journal of Inorganic Biochemistry, 98 (6). 969-976, 2004.
 
[41]  Childs, R. E., and W. G. Bardsley, “The steady-state kinetics of peroxidase with 2,2′-azino-di-(3-ethyl-benzthiazoline-6-sulphonic acid) as chromogen,” Biochemical Journal, 145 (1). 93-103, 1975.
 
[42]  Ruiz-Dueñas, F. J., M. Morales, M. Pérez-Boada, T. Choinowski, M. J. Martínez, K. Piontek, and Á. T. Martínez, “Manganese Oxidation Site in Pleurotus eryngii Versatile Peroxidase:  A Site-Directed Mutagenesis, Kinetic, and Crystallographic Study,” Biochemistry, 46 (1). 66-77, 2007.
 
[43]  Cleland, W. W., “1 Steady State Kinetics,” The Enzymes, D. B. Paul, ed., pp. 1-65: Academic Press, 1970.
 
[44]  Sandler, M., and H. J. Smith, “Introduction to the use of enzyme inhibitors as drugs,” Design of Enzyme Inhibitors as Drugs, , M. Sandler and H. J. Smith, eds., pp. 1-18, Oxford, UK: Oxford University Press, 1989.
 
[45]  Blake, R. D., Informational biopolymers of genes and gene expression, Sausalito, CA: University Science, 2004.
 
[46]  Millero, F. J., Chemical Oceanography, Fourth Edition, Boca Raton: CRC Press, 2013.
 
[47]  Sarkar, S., A. T. Martı́nez, and M. a. J. Martı́nez, “Biochemical and molecular characterization of a manganese peroxidase isoenzyme from Pleurotus ostreatus,” Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1339 (1). 23-30, 1997.
 
[48]  Liu, C., and H. Xu, “The metal site as a template for the metalloprotein structure formation,” Journal of Inorganic Biochemistry, 88 (1). 77-86, 2002.
 
[49]  Emeléus, H. J., and J. S. Anderson, “Modern Aspects of Inorganic Chemistry,” 1956.
 
[50]  Arnold, F. H., and J.-H. Zhang, “Metal-mediated protein stabilization,” Trends in Biotechnology, 12 (5). 189-192, 1994.
 
[51]  Ufot, U. F., and M. I. Akpanabiatu, “An engineered Phlebia radiata manganese peroxidase: expression, refolding, purification and preliminary characterization,” American Journal of Molecular Biology, 2 (4). 359-370, 2012.
 
[52]  Ite, A. E., I. I. Udousoro, and U. J. Ibok, “Distribution of Some Atmospheric Heavy Metals in Lichen and Moss Samples Collected from Eket and Ibeno Local Government Areas of Akwa Ibom State, Nigeria,” American Journal of Environmental Protection, 2 (1). 22-31, 2014.
 
[53]  Ite, A. E., N. F. Hanney, and K. T. Semple, “The Effect of Hydroxycinnamic Acids on the Microbial Mineralisation of Phenanthrene in Soil,” International Journal of Environmental Bioremediation & Biodegradation, 3 (2). 40-47, 2015.
 
[54]  Ufot, U. F., “Expression and Characterisation of a Novel Manganese Peroxidise from Phlebia radiata,” Department of Biochemistry, University of Sussex, University of Sussex, Brighton, United Kingdom, 2010.
 
[55]  Johnson, F., G. H. Loew, and P. Du, “Prediction of Mn(II) binding site of manganese peroxidase from homotology modeling,” Plant peroxidases: Biochemistry and Physiology: III International Symposium 1993: proceedings, xiii, 497 p., K. G. Weirder, S. K. Rasmussen, C. Penel and H. Greppin, eds., pp. 31 – 34, Geneva, Switzerland: University of Copenhagen and University of Geneva, 1993.
 
[56]  Harris, R. Z., H. Wariishi, M. H. Gold, and P. R. Ortiz de Montellano, “The catalytic site of manganese peroxidase. Regiospecific addition of sodium azide and alkylhydrazines to the heme group,” Journal of Biological Chemistry, 266 (14). 8751-8758, 1991.
 
[57]  Whitwam, R. E., K. R. Brown, M. Musick, M. J. Natan, and M. Tien, “Mutagenesis of the Mn2+-Binding Site of Manganese Peroxidase Affects Oxidation of Mn2+ by both Compound I and Compound II,” Biochemistry, 36 (32). 9766-9773, 1997.
 
[58]  Da Silva, J. F., and R. J. P. Williams, The Biological Chemistry of the Elements: the Inorganic Chemistry of Life: Oxford University Press, 2001.
 
[59]  Sunda, W. G., and S. A. Huntsman, “Effect of competitive interactions between manganese and copper on cellular manganese and growth in estuarine and oceanic species of the diatom Thalassiosira,” Limnology and Oceanography, 28 (5). 924-934, 1983.
 
[60]  Sunda, W. G., and S. A. Huntsman, “Relationships among growth rate, cellular manganese and manganese transport kinetics in estuarine and oceanic species of the diatom Thalassiosira,” Journal of Phycology, 22 (3). 259-270, 1986.
 
[61]  Sunda, W. G., “Trace Metal Interactions with Marine Phytoplankton,” Biological Oceanography, 6 (5-6). 411-442, 1989.
 
[62]  Coolbear, T., J. M. Whittaker, and R. M. Daniel, “The effect of metal ions on the activity and thermostability of the extracellular proteinase from a thermophilic Bacillus, strain EA.1,” Biochemical Journal, 287 (Pt 2). 367-374, 1992.
 
[63]  Nieboer, E., and D. H. S. Richardson, “The replacement of the nondescript term ‘heavy metals’ by a biologically and chemically significant classification of metal ions,” Environmental Pollution Series B, Chemical and Physical, 1 (1). 3-26, 1980.
 
[64]  Haas, K. L., and K. J. Franz, “Application of Metal Coordination Chemistry to Explore and Manipulate Cell Biology,” Chemical Reviews, 109 (10). 4921-4960, 2009.
 
Show Less References