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American Journal of Medical and Biological Research

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ISSN (Online): 2328-4099

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Article

Role of Some Metal Ions on Steady–state Kinetics of Engineered Wild–type and Manganese (II) Binding Site Mutants of Recombinant Phlebia radiata Manganese Peroxidase 3 (rPr-MnP3)

1Department of Biological Sciences, Akwa Ibom State University, P.M.B. 1167, Uyo, Akwa Ibom State, Nigeria

2Department of Chemistry, Akwa Ibom State University, P.M.B. 1167, Uyo, Akwa Ibom State, Nigeria

3Research and Development Unit, Akwa Ibom State University, P.M.B. 1167, Uyo, Akwa Ibom State, Nigeria

4Department of Biochemistry, University of Uyo, P. M. B. 1017, Uyo, Akwa Ibom State, Nigeria


American Journal of Medical and Biological Research. 2016, 4(3), 42-52
doi: 10.12691/ajmbr-4-3-2
Copyright © 2016 Science and Education Publishing

Cite this paper:
Usenobong F. Ufot, Aniefiok E. Ite, Idorenyin H. Usoh, Monday I. Akpanabiatu. Role of Some Metal Ions on Steady–state Kinetics of Engineered Wild–type and Manganese (II) Binding Site Mutants of Recombinant Phlebia radiata Manganese Peroxidase 3 (rPr-MnP3). American Journal of Medical and Biological Research. 2016; 4(3):42-52. doi: 10.12691/ajmbr-4-3-2.

Correspondence to: Usenobong  F. Ufot, Department of Biological Sciences, Akwa Ibom State University, P.M.B. 1167, Uyo, Akwa Ibom State, Nigeria. Email: usen_mboso@yahoo.com

Abstract

This study investigated the steady-state kinetics of engineered wild-type and manganese (II) binding site mutants of recombinant Phlebia radiata manganese peroxidase 3(rPr-MnP3). The effect (activation or inhibition) of some metal ions (Co2+, Zn2+ Cu2+ and Na+) on the activity of rPr-MnP3 enzymes was also studied. The results obtained showed that the rPr-MnP3 mutants in which the metal binding functionality has been largely lost have been created. Na+ (mono-valent ion) and Co2+showed similar characteristics by exhibiting stimulatory effects on the activity of wild-type rPr-MnP3. However, Cu2+ and Zn2+ had mixed inhibitory effects on wild-type and mutants (E40H, E44H, E40H/E44H). It was observed that Cu2+ was by far the strongest inhibitor of engineered rPr-MnP3 enzymes while Co2+ exhibited a non-competitive inhibitory effect on the double mutant (E40H/E44H) and D186H activities. In addition, Zn2+ and Cu2+also had non-competitive inhibitory effect on D186H mutant enzyme activity. The results obtained further showed that the competitive inhibitory effect of Cu2+observed in other rPr-MnP3 enzymes is largely removed in D186H mutant enzyme. Generally, histidine substitution retained a strong selectivity for Cu2+ as competitive inhibitor. Zn2+ being generally non-competitive suggest involvement of sites other than the Mn (II) binding site. This study showed that rPr-MnP3 enzymes function with alternate ligands in the Mn2+ binding site and does not have absolute obligate requirement for all carboxylate ligand set.

Keywords

References

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Article

Histomorphometrical Characterization of Skin of Native Cattle (Bos indicus) in Bangladesh

1Department of Quality Assurance, Incepta Vaccine Limited, Dhaka, Bangladesh

2Department of Anatomy and Histology, Chittagong Veterinary and Animal Sciences University, Chittagong, Bangladesh

3Department of Microbiology and Parasitology, Sher-e-Bangla Agricultural University, Dhaka

4Animal Health research Division, Bangladesh Livestock Research Institute, Savar, Dhaka-1341, Bangladesh


American Journal of Medical and Biological Research. 2016, 4(3), 53-65
doi: 10.12691/ajmbr-4-3-3
Copyright © 2016 Science and Education Publishing

Cite this paper:
Md. Ershad Hossain, Mohi Uddin, S. Kumar Shil, M. H. B. kabir, M. Showkat Mahmud, Kh. Nurul Islam. Histomorphometrical Characterization of Skin of Native Cattle (Bos indicus) in Bangladesh. American Journal of Medical and Biological Research. 2016; 4(3):53-65. doi: 10.12691/ajmbr-4-3-3.

Correspondence to: M.  H. B. kabir, Department of Microbiology and Parasitology, Sher-e-Bangla Agricultural University, Dhaka. Email: hazzazpara12sau@gmail.com

Abstract

Fresh skin samples of the native cattle (Bos indicus) of 6 male and 6 female were collected from ten regions of the body immediately after slaughtering aimed to know the regional and sex variation of skin components. The skin samples were fixed, processed and stained according to the standard histological procedure. The samples of vertical sections were stained by both Hematoxylin & Eosin stain and Van Geison & Verhoff’s stain. Transparent sheet and ocular micrometer was used for histomorphometric study. Histomorphometrical study of the skin revealed that the mean thicknesses of epidermis, papillary layer, reticular layer and total skin were 45.37 ± 0.59 µm, 0.55 ± 0.01 mm, 3.03 ± 0.09 mm and 3.57 ± 0.09 mm respectively. These thicknesses were significantly (P<0.05) varied among the body regions. The highest thicknesses for epidermis and papillary layer were found in back and ventral abdomen respectively and the lowest thicknesses in thigh and neck & shoulder respectively whereas the highest thicknesses for reticular layer and total skin were found in head and the lowest thicknesses in shoulder. The mean thicknesses of the epidermis, reticular layer and total skin were significantly (P<0.05)) higher in male than the female. The mean papillary layer thickness was insignificantly (P<0.05) higher in female than the male. The mean density of collagen fibre bundle, elastic fibre bundle, sebaceous gland, sweat gland and hair follicle per mm² were 302.93 ± 9.07, 4.96 ± 0.17, 4.22 ± 0.13, 4.57 ± 0.14 and 37.25 ± 1.77 respectively. The density of collagen and elastic fibre bundle were significantly (P<0.05) varied among the body regions. The highest density of collagen bundle was found in ventral abdomen and lowest density in thigh and the highest elastic bundle density in thigh and lowest in back & ventral abdomen. The density of collagen fibre, hair follicle and sebaceous gland were significantly higher (P<0.05) in male than the female. The density of sebaceous gland was significantly (P<0.05) highest in head and lowest in lateral abdomen. The density of sweat gland was insignificantly (P>0.05) highest in neck & shoulder and lowest in loin. The density of hair follicle was significantly (P<0.05) highest in back and lowest in ventral abdomen. In conclusion, male skin may be better than the female skin for the quality leather production. Collagen bundle is more compact in ventral abdomen that regions may be better for quality leather production, but the percentage of collagen did not revealed in this study.

Keywords

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Article

A Study of Possible Association between Cannabinoid Receptor Gene II and Drug Dependence

1Department of Forensic Medicine and Clinical Toxicology, Faculty of Medicine, Alexandria University, Egypt

2Currently working at College of Biotechnology, University of Modern Sciences, Dubai


American Journal of Medical and Biological Research. 2016, 4(4), 66-72
doi: 10.12691/ajmbr-4-4-1
Copyright © 2016 Science and Education Publishing

Cite this paper:
Fouad, R. Gomaa, M. Farag. A Study of Possible Association between Cannabinoid Receptor Gene II and Drug Dependence. American Journal of Medical and Biological Research. 2016; 4(4):66-72. doi: 10.12691/ajmbr-4-4-1.

Correspondence to: R.  Gomaa, Department of Forensic Medicine and Clinical Toxicology, Faculty of Medicine, Alexandria University, Egypt. Email: drgomaa14@gmail.com

Abstract

Drug dependence is considered a major contributor to both medical morbidity and mortality all over the world. It also represents an important health problem that has a great impact on the person's life both socially and economically. It was suggested that there is a substantial genetic contribution to drug dependence vulnerability. Cannabinoid receptors are known to be activated by natural as well as synthetic cannabinoids. Several evidences suggested that improved information about Cannabinoid receptor genes and their human variants might add to the understanding of vulnerabilities to drug dependence. The current study aimed at investigating the possible association between the cannabinoid receptor gene and drug dependence. The study was conducted on 150 drug dependent individuals. The diagnosis of drug dependence was based on the current Diagnostic and Statistical Manual of Mental disorders (DSM-IV) and urine screening tests. These individuals were using either Cannabis or Tramadol solely or in combination. All drug dependent individuals were males and all were current smokers. The duration of drug abuse ranged from 1 to 9 years. All participants were screened for a nucleotide polymorphism in cannabinoid receptor 2 gene (CB2) by PCR amplification and HapII Restriction Fragment Length Polymorphism analysis. The study has proved a significant association between occurrence of polymorphism in the Cannabinoid Receptor 2 gene and drug dependence, where 83.3% of drug dependents showed the polymorphism compared to 15% of the control group. A significant association was also detected between the presence of this polymorphism and family history of drug dependence and.The results of the present study confirmed the possible role of Cannabinoid Receptor 2 gene in drug dependence vulnerability.

Keywords

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