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ISSN (Print): 2328-4080

ISSN (Online): 2328-4099

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Website: http://www.sciepub.com/journal/AJMBR



Analysis of expression of mRNA Matrix Metalloproteinase (MMP)-8 gene and Tissue Inhibitors of Metalloproteinase (MMP)-1 in Intraperitoneal Adhesion after Usage of Hyaluronate acid-Carboxymethylcellulose (HA-CMC) or Virgin Coconut Oil (VCO)

1Department of Surgery, Faculty of Medicine, Sam Ratulagi University, Manado, Indonesia

2Department of Surgery, Faculty of Medicine, Indonesia University, Jakarta, Indonesia

3Department of Surgery, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia

4Molecular Biology and Immunology Laboratory, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia

American Journal of Medical and Biological Research. 2015, 3(4), 113-116
doi: 10.12691/ajmbr-3-4-6
Copyright © 2015 Science and Education Publishing

Cite this paper:
Jimmy Panelewen, Aryono Djuned Pusponegoro, Ibrahim Labeda, Andi Asadul Islam, Mochammad Hatta. Analysis of expression of mRNA Matrix Metalloproteinase (MMP)-8 gene and Tissue Inhibitors of Metalloproteinase (MMP)-1 in Intraperitoneal Adhesion after Usage of Hyaluronate acid-Carboxymethylcellulose (HA-CMC) or Virgin Coconut Oil (VCO). American Journal of Medical and Biological Research. 2015; 3(4):113-116. doi: 10.12691/ajmbr-3-4-6.

Correspondence to: Mochammad  Hatta, Molecular Biology and Immunology Laboratory, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia. Email: hattaram@indosat.net.id


Background: Purpose : To understand the expression of matrix metalloproteinase (MMP)-8 mRNA gene and tissue inhibitors of metalloproteinase (TIMP)-1 in normal peritoneum tissue and intraperitoneal adhesion tissue after the usage of virgin coconut oil (VCO) and hyaluronate Acid-carboxymethylcellulose (HA-CMC). Methods: The research is using an experimental lab rat “rattus norvegicus strain wistar” which is divided into 3 groups. Laparotomy (pre) is done to gain parietal peritoneum tissue at the lower left quadrant of the abdomen, in order to obtain the expression of mRNA gene MMP-8 and TIMP-1. First group was control group, second group was treated with VCO, while the third group was treated with HA-CMC. After two weeks period, another relaparotomy (post) was held to obtain the adhesion tissue and to run another examination on the MMP-8 mRNA gene expression and TIMP-1. Results: There is a meaningful decrease for the MMP-8 mRNA gene expression in the peritoneum tissue for the control group at the pre and post laparotomy (p<0,001). There is a meaningful over expression of TIMP-1 mRNA gene expression at pre dan post laparatomy of control group (p<0,001). While on the other hand, at the VCO nor HA-CMC group, there is an insignificant increase of MMP-8 mRNA gene expression (p=0,075 and p=0,791) and insignificant decrease of TIMP-1 mRNA gene expression (p=0,015 and p=0,368). Conclusion: The decrease of MMP-8 mRNA gene expression and the increasing of TIMP-1 mRNA gene expression of control group shows a high risk of adhesion incidence. Although it is not statistically meaningful, the result of MMP-8 mRNA and TIMP-1 mRNA gene expression of VCO and HA-CMC indicates that there is a decrease of intraperitoneal adhesion risks, and the nature of VCO anti adhesive compared to HA-CMC.



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Osteometric Assessment of the Mastoids for Gender Determination in Jordanians by Discriminant Function Analysis

1Department of Prosthodontics, Faculty of Dentistry, University of Jordan, West Amman Province 11942, Amman, Jordan.

2Postgraduate Section, Department of Prosthodontics, Faculty of Dentistry, University of Jordan, Wes Amman Province 11942, Amman, Jordan

3Intern Dental Practitioner, Jordan University Hospital, West Amman Province 11942, Amman, Jordan

American Journal of Medical and Biological Research. 2015, 3(4), 117-123
doi: 10.12691/ajmbr-3-4-7
Copyright © 2015 Science and Education Publishing

Cite this paper:
Amin W, Saleh M-W, Othman D, Salhab D, Thunaibat H.. Osteometric Assessment of the Mastoids for Gender Determination in Jordanians by Discriminant Function Analysis. American Journal of Medical and Biological Research. 2015; 3(4):117-123. doi: 10.12691/ajmbr-3-4-7.

Correspondence to: Amin  W, Department of Prosthodontics, Faculty of Dentistry, University of Jordan, West Amman Province 11942, Amman, Jordan.. Email: wami@ju.edu.jo, walaamin@gmail.com


The purpose of this study was, firstly, to characterize the mastoid process in terms of its size, its surface area, its flare and its medial convergence angle on a sample of Jordanian subjects; and secondly, to assess the validity of the mastoid process in determining the gender of the study subjects. The sample of the study comprised 192 3D skull images with known sex, 96 each male and female, those were obtained by rendering DICOM images from cone-beam computerized tomography of the subjects. Radiographic measurements, using customized software, accurately characterized the subjects’ mastoids. Discriminant function analysis revealed that mastoid process correctly classified the sex in 90.6 percent of the subjects and the intermastoidale distance was found to be the best determinant for sex. A discriminant function equation specific for young and middle age Jordanians has been derived from the mastoid variables.



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Cissus populnea (Guill & Perr): A Study of the Aqueous Extract as Potential Spermatogenic Enhancers in Male Wistar Rats

1Department of Biochemistry, Lagos State University, PMB 0001 LASU Post Office, Ojo, Lagos, Nigeria

2Department of Chemistry, Lagos State University, PMB 0001 LASU Post Office, Ojo, Lagos, Nigeria

3Department of Anatomy, Lagos State University College of Medicine, P.M.B. 21266, Ikeja, Lagos, Nigeria

American Journal of Medical and Biological Research. 2015, 3(5), 124-127
doi: 10.12691/ajmbr-3-5-1
Copyright © 2015 Science and Education Publishing

Cite this paper:
Anthony B. Ojekale, Oladipupo A. Lawal, Peter I. Jewo, Jamiu. A. Oguntola, Latifat O. Abdul. Cissus populnea (Guill & Perr): A Study of the Aqueous Extract as Potential Spermatogenic Enhancers in Male Wistar Rats. American Journal of Medical and Biological Research. 2015; 3(5):124-127. doi: 10.12691/ajmbr-3-5-1.

Correspondence to: Anthony  B. Ojekale, Department of Biochemistry, Lagos State University, PMB 0001 LASU Post Office, Ojo, Lagos, Nigeria. Email: anthony.ojekale@lasu.edu.ng


Background: There is a global observed decline in male fertility over the years consequent on various factors affecting spermatogenesis. Some Nigerian males across various geographic and socio-economic strata employ the use of herbals such as Cissus populnea extracts amongst others to manage this and also as herbal aphrodisiacs. This study assessed the spermatogenic effects of aqueous extract of Cissus populnea stem bark. Methodology: Cissus populnea stem bark was extracted with water, concentrated and lyophilized. Male wistar rats were orally administered with 150 mg/kg body weight of aqueous extract (Cissus populnea) over a 64 day period. Sperm of the experimental animals was collected, and the parameters (count, motility, morphology) analysed. Data were analysed with SPSS 19. Principal findings: The oral administration of C. populnea extract over a 64 day period to male wistar rats resulted in a four-fold increase in sperm count in the test rats (145±55) compared with the control group (44±17). Testicular histology shows better packed spermatozoa in group of rats treated with Cissus populnea. Conclusion/significance: The findings suggest that oral administration of Cissus populnea aqueous extract improves spermatogenesis in male wistar rats.



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