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American Journal of Medical and Biological Research

ISSN (Print): 2328-4080

ISSN (Online): 2328-4099

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Website: http://www.sciepub.com/journal/AJMBR

   

Article

Impact of Nigella Sativa, Omega-3 Fatty Acids and Chromium Picolinate on NF-κB /leptin-insulin Axis in Obese Subjects with Non-alcoholic Fatty Liver Disease

1Departments of Medical Biochemistry, Faculty of Medicine, Tanta University, Egypt

2Department of Clinical Biochemistry and Molecular Medicine, Taibah Faculty of Medicine, Taibah University, Al-Madinah Al-Munawwarah, Saudi Arabia

3Ph.D in Medical Biochemistry, Faculty of Medicine, Tanta University, Egypt

4Department of Internal Medicine, Faculty of Medicine, Tanta University, Egypt

5Department of Medical Biochemistry, Sohag Faculty of Medicine, Sohag University, Egypt


American Journal of Medical and Biological Research. 2015, 3(6), 139-145
doi: 10.12691/ajmbr-3-6-1
Copyright © 2015 Science and Education Publishing

Cite this paper:
Noha M. Shafik, Reham A. Mariah, Sherine Ragab Shafik, Loai Mohammed El-Ahwal, Salah Mohamed El Sayed. Impact of Nigella Sativa, Omega-3 Fatty Acids and Chromium Picolinate on NF-κB /leptin-insulin Axis in Obese Subjects with Non-alcoholic Fatty Liver Disease. American Journal of Medical and Biological Research. 2015; 3(6):139-145. doi: 10.12691/ajmbr-3-6-1.

Correspondence to: Salah  Mohamed El Sayed, Department of Clinical Biochemistry and Molecular Medicine, Taibah Faculty of Medicine, Taibah University, Al-Madinah Al-Munawwarah, Saudi Arabia. Email: salahfazara@yahoo.com, drsalahpediatr@yahoo.com

Abstract

Background: The global problem of obesity epidemic involves an increased risk of non-alcoholic fatty liver disease (NAFLD) whereby oxidative stress induces fibrogenesis. Objectives: to assess the coordinated impact of obesity management strategy on nuclear factor kappa beta/p65 mRNA expression and biochemical parameters of oxidative stress, glycemic status and adipokines. Subjects and methods: This study was carried out on 60 subjects classified into, 15 normal healthy controls (group І) and 45 obese subjects who were divided equally into three groups: obese subjects with NAFLD (group ІІ), obese subjects who received natural product supplements (nigella sativa, green tea andchromium picolinate) (groupІІІ),obese subjects who received omega-3 fatty acids (group ІV) andthose who received caloric restricted diet (CRD) and exercise for 6 months. All groups were subjected to measurement of body mass index, waist to hip ratio (WHR), spectrophotometric measurement of serum levels of glucose, malondialdehyde (MDA) and total antioxidant capacity (AOC%), NF-κB /P65 subunit expression levels by real time-PCR in peripheral bloodmononuclear cells, estimation of levels of insulin, insulin like growth factor-1, leptin, adiponectin, fibronectin and oxidized LDL by enzyme linked immunosorbent assays. Results: There was a significant decrease inNF-κB/p65 mRNA expression in peripheral blood mononuclear cells, reduction in the levels of oxLDL, decreased insulin resistance and decreased leptin resistance which might be linked to hypoadiponectinemia. Levels of (AOC %) were significantly elevated after treatment. This was evident alongside reduction of BMI, WHR and fibrogenic potential in NAFLD. Conclusion: natural product supplements, CRD and exercise ameliorated the fibrogenic and atherogenic consequences of immune-inflammatory and oxidative stress-induced pathological mechanisms associated with obesity.

Keywords

References

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Article

Soybean Protective Effect to 8-OHdG on UVB Induced-Hairless Mice

1Daya Regional Public Hospital of Makassar, South Sulawesi, Indonesia

2Hasanuddin University, Department of Microbiology, Faculty of Medicine, Jl. PerintisKemerdekaan Km 10 Tamalanrea, Makassar, South Sulawesi, Indonesia

3Hasanuddin University, Department of Pharmacognosis Phytochemistry, Faculty of Pharmacy, Jl. PerintisKemerdekaan Km 10 Tamalanrea, Makassar, South Sulawesi, Indonesia

4Hasanuddin University, Department of Dermatovenereology, Faculty of Medicine, Jl. PerintisKemerdekaan Km 10 Tamalanrea, Makassar, South Sulawesi, Indonesia


American Journal of Medical and Biological Research. 2015, 3(6), 146-148
doi: 10.12691/ajmbr-3-6-2
Copyright © 2015 Science and Education Publishing

Cite this paper:
Zainuddin AS, Massi MN, Alam G, Djawad K, Yusuf I. Soybean Protective Effect to 8-OHdG on UVB Induced-Hairless Mice. American Journal of Medical and Biological Research. 2015; 3(6):146-148. doi: 10.12691/ajmbr-3-6-2.

Correspondence to: Zainuddin  AS, Daya Regional Public Hospital of Makassar, South Sulawesi, Indonesia. Email: amirahshaleha@gmail.com

Abstract

Aims. TheUVB light radiation inducts 8-OHdG as the photoproduct in which resulting the DNA impairment through stress oxidative pathway. Genistein is one of the basic component from soybean that the isoflavone inside conducts strong antioxidant effect. This study main purpose is to see the application of genistein’s photoprotective instrument on UVB induced-hairless mice. Method. 24 albino mice, age 6-9 weeks, weight 15-25 grams were used as the sample of the study. These mice were divided into 6 different way of treating groups, group I as the control group which had been selected as the negative control—untreated ones, group II as the control group which had the UVB radiation, group III as UVB radiation control group with Aceton-based, group IV which had 100 ppm genistein. Group V had 200 ppm genistein and group VI had UVB radiation 3 times a week with 500 mJ dosage for 12 weeks. After 24 hours of the last UV light exposure giving, these mice were killed off to aim the skin biopsy for 8-OHdG laboratory examination. Result. 8-OhdG level at 100 ppm genistein control group was the lowest one rather than the UVB radiation and 400 ppm control group. And, it’s not significantly showed at 200 ppm genistein control group. Conclusion. 100 ppm, 200 ppm and 400 ppm of genistein application showed the protective effect to 8-OHdG on UVB induced-hairless mice, which the best dose usage was 100 ppm of genistein.

Keywords

References

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Article

Achieving Pregnancy in Situational Psychogenic Anejaculation in an Islamic Community - Two Case Reports and Literature Review

1Department of Urology, Alexandria faculty of Medicine, Alexandria, Egypt


American Journal of Medical and Biological Research. 2015, 3(6), 149-151
doi: 10.12691/ajmbr-3-6-3
Copyright © 2016 Science and Education Publishing

Cite this paper:
Nader Salama. Achieving Pregnancy in Situational Psychogenic Anejaculation in an Islamic Community - Two Case Reports and Literature Review. American Journal of Medical and Biological Research. 2015; 3(6):149-151. doi: 10.12691/ajmbr-3-6-3.

Correspondence to: Nader  Salama, Department of Urology, Alexandria faculty of Medicine, Alexandria, Egypt. Email: nadersalama58@yahoo.com

Abstract

Introduction: Psychogenic anejaculation is a rare cause of male infertility. In this type of anejaculation, the patient cannot ejaculate while awake. Most cases of this disorder were reported in Jewish communities. In the present report, we describe the successful achievement of pregnancy in two Muslim men with situational psychogenic anejaculation. The treatment involved the simplest approach in one case but the highly technical procedure in the second case. Case presentation: Two men presented with situational psychogenic anejaculation and concern to conceive. Clinical examination, hormonal assay, urine analysis and ultrasound imaging identified irrelevant data in both. Psychosexual counseling was rejected by both men and penile vibration did not give a response in either. Case 1 succeeded to get sperm from masturbation-induced ejaculate. Case 2 got sperm after doing conventional testicular sperm extraction. The use of the retrieved sperms succeeded to attain pregnancy in both cases. However, the anejaculationdid not revert in either patient. Conclusion: Men from Islamic communities with strict religious upbringing have the possibility to have psychogenic anejaculation. This report confirms the good fertilizing potential of sperm and its capability of achieving pregnancy in men with psychogenic anejaculation. Men with this type of anejaculation are resistant to improvement even after their concern to conceive dissipates.

Keywords

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