ISSN (Print): 2328-7322

ISSN (Online): 2328-7314


Editor-in-chief: Nabil Abdel-Hamid

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Currrent Issue: Volume 4, Number 3, 2016


Hepatoprotective Effects Exerted by Propolis against Doxorubicin-induced Rat Liver Toxicity: A Biochemical and Histopathological Study

1Department of Anatomy, Faculty of Clinical Pharmacy, Taif University, KSA

2Department of Anatomy, Faculty of Medicine, Sohag University, Egypt

3Department of Pathology, Al-Ghad International Medical Sciences Colleges, Al-Madinah, KSA

4Department of Pathology, Faculty of Medicine, Sohag University, Egypt

5Department of Clinical Biochemistry and Molecular Medicine, Taibah Faculty of Medicine, Taibah University, Al-Madinah Al-Munawwarah, Saudi Arabia

6Department of Pediatrics, College of Medicine, Taibah College of Medicine, Taibah University, Al-Madinah Al-Munawwarah, Saudi Arabia

7Department of ear, nose and throat surgery, Taibah Faculty of Medicine, Taibah University, Al-Madinah Al-Munawwarah, Saudi Arabia

8Department of Pediatrics, Sohag Teaching Hospital, Sohag, Egypt

9Division of Pediatric cardiology, Department of Pediatrics, Maternity and Children Hospital, King Abdullah Medical City, Al-Madinah Al-Munawwarah, Kingdom of Saudi Arabia

10Department of Clinical Pathology, Faculty of Medicine, 6th October University, Cairo, Egypt

11Department of Medical Biochemistry, Sohag Faculty of Medicine, Sohag University, Egypt

American Journal of Cancer Prevention. 2016, 4(3), 36-40
doi: 10.12691/ajcp-4-3-1
Copyright © 2016 Science and Education Publishing

Cite this paper:
Nassar Ayoub Abdellatif Omar, Amal Nor Edeen Ahmad Allithy, Hussam Baghdadi, Mohammed Zolaly, Mohamed Abdel-haleem, Manal Mohamed Helmy, Mongi Mohamed Ahmed Ayat, Salah Mohamed El Sayed. Hepatoprotective Effects Exerted by Propolis against Doxorubicin-induced Rat Liver Toxicity: A Biochemical and Histopathological Study. American Journal of Cancer Prevention. 2016; 4(3):36-40. doi: 10.12691/ajcp-4-3-1.

Correspondence to: Salah  Mohamed El Sayed, Department of Clinical Biochemistry and Molecular Medicine, Taibah Faculty of Medicine, Taibah University, Al-Madinah Al-Munawwarah, Saudi Arabia. Email:


Doxorubicin (DOX) is a currently used broad spectrum anticancer agent used to treat many cancer types. DOX belongs to anthracycline group of cytotoxic drugs. Unfortunately, DOX induces many side effects e.g. cardiotoxicity, hepatotoxicity and nephrotoxicity. In this experimental study, we assessed the protective potential of honey bee propolis against DOX-induced side effects. Thirty Sprague Dawley male rats (weighing about 200-220 g) were included in our study and divided into three equal experimental groups: group I (untreated control), group II (DOX-treated rats) that received 25 mg/kg DOX by intraperitoneal (i.p) injection for three consecutive days and group III where animals received both DOX and propolis oral propolis (250 mg/kg for 30 consecutive days). By the end of experimental time, all animals were sacrificed on the 30th day where blood samples and tissue sections were collected for detection of the biochemical and histopathological changes. Our data revealed that propolis evidently resulted in hepatoprotective effects against DOX-induced toxicity in rats liver. It can be concluded that propolis provides partial protection against DOX-induced hepatotoxicity.



[1]  Zordoky BN, Anwar-Mohamed A, Aboutabl ME, El-Kadi AO. Acute doxorubicin toxicity differentially alters cytochrome P450 expression and arachidonic acid metabolism in rat kidney and liver. Drug Metab Dispos. 2011 Aug; 39(8): 1440-50.
[2]  Outomuro D, Grana DR, Azzato F, Milei J. Adriamycin-induced myocardial toxicity: new solutions for an old problem? Int J Cardiol 2007; 117: 6-15.
[3]  Injac R, Strukelji B. Recent advances in protection against doxorubicin-induced toxicity. Technology in Cancer Research Treatment, 2008; 7: 497-516.
[4]  Deepa PR, Varalakshmi P. Biochemical evaluation of the inflammatory changes in cardiac, hepatic and renal tissues of Adriamycin-administered rats and the modulatory role of exogenous heparin-derivative treatment. Chem Biol Interact 2005; 156: 93-100.
[5]  Bulucu F, Ocal R, Karadurmus N, Sahin M, Kenar L, Aydin A, Oktenli C, Koc B, Inal V, Yamanel L et al. Effects of N-acetylcysteine, deferoxamine and selenium on doxorubicin-induced hepatotoxicity. Biol Trace Elem Res, 2009; 132: 184-196.
Show More References
[6]  Boutabet K, Kebsa W., Alyane M., Lahouel M Polyphenolic fraction of Algerian propolis protects rat kidney against acute oxidative stress induced by doxorubicin. Indian J Nephrol. 2011 Apr-Jun; 21(2): 101-106.
[7]  Singla S, Neelima R. Kumar, and Jaspreet KaurIn vivo Studies on the Protective Effect of Propolis on Doxorubicin-Induced Toxicity in Liver of Male Rats. Toxicol Int. 2014 May-Aug; 21(2): 191-195.
[8]  Kaiserová H1, Simůnek T, van der Vijgh WJ, Bast A, Kvasnicková E. Flavonoids as protectors against doxorubicin cardiotoxicity: role of iron chelation, antioxidant activity and inhibition of carbonyl reductase. Biochim Biophys Acta. 2007 Sep;1772(9):1065-74.
[9]  Bachur NR. Anthracycline antibiotic pharmacology and metabolism. Cancer Treat Rep. 1979 May;63(5):817-20.
[10]  Thorn CF1, Oshiro C, Marsh S, Hernandez-Boussard T, McLeod H, Klein TE, Altman RB. Pharmacogenet Genomics. 2011 Jul;21(7):440-6. Doxorubicin pathways: pharmacodynamics and adverse effects.
[11]  REITMAN S, FRANKEL S. A colorimetric method for the determination of serum glutamic oxalacetic and glutamic pyruvic transaminases. Am J Clin Pathol. 1957 Jul;28(1):56-63.
[12]  Peterson BT, Tate RW.Albumin fraction and measurement of total protein. concentration. Am J Physiol. 1993 May; 264(5 Pt 2): H1723-6.
[13]  Kosalec I, Bakmaz M, Pepeljnjak S, Vladimir-Knezevic S. Quantitative analysis of the flavonoids in raw propolis from northern Croatia. Acta Pharm. 2004; 54: 65-72.
[14]  Inderber Singh. Textbook of human histology. 6th edition. Jaypee Brothers Medical Publishers, 2011 Pages: 263-270.
[15]  Eraslan G, Kanbur M, Silici S, Altinordulu S, Karabacak M. Effects of cypermethrin on some biochemical changes in rats: The protective role of propolis. Exp Anim. 2008; 57:453-60.
[16]  Barrera G. Oxidative stress and lipid peroxidation products in cancer progression and therapy. ISRN Oncol. 2012; 2012: 137289.
[17]  Sun LP, Xu X, Hwang HH, Wang X, Su KY, Chen YL. Dichloromethane extracts of propolis protect cell from oxygen-glucose deprivation-induced oxidative stress via reducing apoptosis. Food Nutr Res. 2016 Jun 20; 60: 30081.
Show Less References